Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients
Specific and non- specific cellular immune responses were studied in 20 amoebiasis cases and 10 controls. All the study cases and controls were Indians living in Varanasi, India. Ten amoebic liver abscess cases were patients admitted to University hospital. Ten non-invasive intestinal amoebiasis cas...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2004-03-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/1926 |
| _version_ | 1818729165174603776 |
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| author | N Arianpour TM Mohapatra |
| author_facet | N Arianpour TM Mohapatra |
| author_sort | N Arianpour |
| collection | DOAJ |
| description | Specific and non- specific cellular immune responses were studied in 20 amoebiasis cases and 10 controls. All the study cases and controls were Indians living in Varanasi, India. Ten amoebic liver abscess cases were patients admitted to University hospital. Ten non-invasive intestinal amoebiasis cases constituted the second study group. Non-specific immune responses were studied using E-rosette technique. Specific cellular immune response was evaluated by measuring tritiated thymidine uptake by transformed lymphocytes using crude amoebic extract prepared from axenically grown Entamoeba histolytica NIH: 200 and two of its chromatographed fractions, i.e. fraction I (FI) & fraction II (FII) as well as a mitogen, i.e. Phyto Haemagglutinin-A (PHA). Results show those crude antigens followed by FI & FII are able to induce blastoid transformation of lymphocytes prepared from different cases. Moreover, it was shown that specific cellular immune response was greatly depressed in amoebic liver abscess cases (P |
| first_indexed | 2024-12-17T22:41:33Z |
| format | Article |
| id | doaj.art-3395a660ef5543eab0d4e725209d512c |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-17T22:41:33Z |
| publishDate | 2004-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-3395a660ef5543eab0d4e725209d512c2022-12-21T21:29:55ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932004-03-01331Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients N Arianpour0 TM Mohapatra1 Specific and non- specific cellular immune responses were studied in 20 amoebiasis cases and 10 controls. All the study cases and controls were Indians living in Varanasi, India. Ten amoebic liver abscess cases were patients admitted to University hospital. Ten non-invasive intestinal amoebiasis cases constituted the second study group. Non-specific immune responses were studied using E-rosette technique. Specific cellular immune response was evaluated by measuring tritiated thymidine uptake by transformed lymphocytes using crude amoebic extract prepared from axenically grown Entamoeba histolytica NIH: 200 and two of its chromatographed fractions, i.e. fraction I (FI) & fraction II (FII) as well as a mitogen, i.e. Phyto Haemagglutinin-A (PHA). Results show those crude antigens followed by FI & FII are able to induce blastoid transformation of lymphocytes prepared from different cases. Moreover, it was shown that specific cellular immune response was greatly depressed in amoebic liver abscess cases (Phttps://ijph.tums.ac.ir/index.php/ijph/article/view/1926Thymidine uptakeSpecific cellular immune responseNon-specific cellular immune responseAmoebiasis |
| spellingShingle | N Arianpour TM Mohapatra Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients Iranian Journal of Public Health Thymidine uptake Specific cellular immune response Non-specific cellular immune response Amoebiasis |
| title | Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients |
| title_full | Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients |
| title_fullStr | Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients |
| title_full_unstemmed | Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients |
| title_short | Evaluation of Specific and Non-Specific Cellular Immune Responses in Amoebiasis Patients |
| title_sort | evaluation of specific and non specific cellular immune responses in amoebiasis patients |
| topic | Thymidine uptake Specific cellular immune response Non-specific cellular immune response Amoebiasis |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/1926 |
| work_keys_str_mv | AT narianpour evaluationofspecificandnonspecificcellularimmuneresponsesinamoebiasispatients AT tmmohapatra evaluationofspecificandnonspecificcellularimmuneresponsesinamoebiasispatients |