Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia
Androgen assessment is a key element for diagnosing polycystic ovary syndrome (PCOS), and defining a “normal” level of circulating androgens is critical for epidemiological studies. We determined the upper normal limits (UNLs) for androgens in a population-based group of premenopausal “healthy contr...
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MDPI AG
2022-12-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/1/33 |
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| author | Larisa Suturina Daria Lizneva Alina Atalyan Ludmila Lazareva Aleksey Belskikh Tatyana Bairova Leonid Sholokhov Maria Rashidova Irina Danusevich Iana Nadeliaeva Lilia Belenkaya Zorikto Darzhaev Eldar Sharifulin Natalia Belkova Ilia Igumnov Tatyana Trofimova Anastasiya Khomyakova Kseniia Ievleva Natalia Babaeva Irina Egorova Madinabonu Salimova Bulent O. Yildiz Richard S. Legro Frank Z. Stanczyk Ricardo Azziz |
| author_facet | Larisa Suturina Daria Lizneva Alina Atalyan Ludmila Lazareva Aleksey Belskikh Tatyana Bairova Leonid Sholokhov Maria Rashidova Irina Danusevich Iana Nadeliaeva Lilia Belenkaya Zorikto Darzhaev Eldar Sharifulin Natalia Belkova Ilia Igumnov Tatyana Trofimova Anastasiya Khomyakova Kseniia Ievleva Natalia Babaeva Irina Egorova Madinabonu Salimova Bulent O. Yildiz Richard S. Legro Frank Z. Stanczyk Ricardo Azziz |
| author_sort | Larisa Suturina |
| collection | DOAJ |
| description | Androgen assessment is a key element for diagnosing polycystic ovary syndrome (PCOS), and defining a “normal” level of circulating androgens is critical for epidemiological studies. We determined the upper normal limits (UNLs) for androgens in a population-based group of premenopausal “healthy control” women, overall and by ethnicity (Caucasian and Asian), in the cross-sectional Eastern Siberia PCOS Epidemiology and Phenotype (ESPEP) Study (ClinicalTrials.gov ID: NCT05194384) conducted in 2016–2019. Overall, we identified a “healthy control” group consisting of 143 healthy premenopausal women without menstrual dysfunction, hirsutism, polycystic ovaries, or medical disorders. We analyzed serum total testosterone (TT) by using liquid chromatography with tandem mass spectrometry (LC-MS/MS), and DHEAS, sex-hormone-binding globulin (SHBG), TSH, prolactin, and 17-hydroxyprogesterone (17OHP) were assessed with an enzyme-linked immunosorbent assay (ELISA). The UNLs for the entire population for the TT, free androgen index (FAI), and DHEAS were determined as the 98th percentiles in healthy controls as follows: 67.3 (95% confidence interval (CI): 48.1, 76.5) ng/dl, 5.4 (3.5, 14.0), and 355 (289, 371) μg/dl, respectively. The study results demonstrated that the UNLs for TT and FAI varied by ethnicity, whereas the DHEAS UNLs were comparable in the ethnicities studied. |
| first_indexed | 2024-03-11T10:04:43Z |
| format | Article |
| id | doaj.art-3395d4c1311f4edea7d4f25ad8397d23 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T10:04:43Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-3395d4c1311f4edea7d4f25ad8397d232023-11-16T15:07:57ZengMDPI AGDiagnostics2075-44182022-12-011313310.3390/diagnostics13010033Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern SiberiaLarisa Suturina0Daria Lizneva1Alina Atalyan2Ludmila Lazareva3Aleksey Belskikh4Tatyana Bairova5Leonid Sholokhov6Maria Rashidova7Irina Danusevich8Iana Nadeliaeva9Lilia Belenkaya10Zorikto Darzhaev11Eldar Sharifulin12Natalia Belkova13Ilia Igumnov14Tatyana Trofimova15Anastasiya Khomyakova16Kseniia Ievleva17Natalia Babaeva18Irina Egorova19Madinabonu Salimova20Bulent O. Yildiz21Richard S. Legro22Frank Z. Stanczyk23Ricardo Azziz24Federal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaFederal State Public Institution “Scientific Center for Family Health and Human Reproduction Problems”, 16, Timiryazeva Str., 664003 Irkutsk, RussiaDivision of Endocrinology and Metabolism, Hacettepe University School of Medicine, Hacettepe, 06100 Ankara, TurkeyHershey Medical Center, Penn State College of Medicine, 500 University Dr, Hershey, PA 17033, USAKeck School of Medicine, University of Southern California, 1975 Zonal Ave, Los Angeles, CA 90033, USASchool of Medicine and Public Health, University of Alabama at Birmingham, 1700 6th Ave, South Birmingham, AL 35249, USAAndrogen assessment is a key element for diagnosing polycystic ovary syndrome (PCOS), and defining a “normal” level of circulating androgens is critical for epidemiological studies. We determined the upper normal limits (UNLs) for androgens in a population-based group of premenopausal “healthy control” women, overall and by ethnicity (Caucasian and Asian), in the cross-sectional Eastern Siberia PCOS Epidemiology and Phenotype (ESPEP) Study (ClinicalTrials.gov ID: NCT05194384) conducted in 2016–2019. Overall, we identified a “healthy control” group consisting of 143 healthy premenopausal women without menstrual dysfunction, hirsutism, polycystic ovaries, or medical disorders. We analyzed serum total testosterone (TT) by using liquid chromatography with tandem mass spectrometry (LC-MS/MS), and DHEAS, sex-hormone-binding globulin (SHBG), TSH, prolactin, and 17-hydroxyprogesterone (17OHP) were assessed with an enzyme-linked immunosorbent assay (ELISA). The UNLs for the entire population for the TT, free androgen index (FAI), and DHEAS were determined as the 98th percentiles in healthy controls as follows: 67.3 (95% confidence interval (CI): 48.1, 76.5) ng/dl, 5.4 (3.5, 14.0), and 355 (289, 371) μg/dl, respectively. The study results demonstrated that the UNLs for TT and FAI varied by ethnicity, whereas the DHEAS UNLs were comparable in the ethnicities studied.https://www.mdpi.com/2075-4418/13/1/33hyperandrogenismandrogensLC-MS/MStestosteroneDHEAScut-off |
| spellingShingle | Larisa Suturina Daria Lizneva Alina Atalyan Ludmila Lazareva Aleksey Belskikh Tatyana Bairova Leonid Sholokhov Maria Rashidova Irina Danusevich Iana Nadeliaeva Lilia Belenkaya Zorikto Darzhaev Eldar Sharifulin Natalia Belkova Ilia Igumnov Tatyana Trofimova Anastasiya Khomyakova Kseniia Ievleva Natalia Babaeva Irina Egorova Madinabonu Salimova Bulent O. Yildiz Richard S. Legro Frank Z. Stanczyk Ricardo Azziz Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia Diagnostics hyperandrogenism androgens LC-MS/MS testosterone DHEAS cut-off |
| title | Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia |
| title_full | Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia |
| title_fullStr | Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia |
| title_full_unstemmed | Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia |
| title_short | Establishing Normative Values to Determine the Prevalence of Biochemical Hyperandrogenism in Premenopausal Women of Different Ethnicities from Eastern Siberia |
| title_sort | establishing normative values to determine the prevalence of biochemical hyperandrogenism in premenopausal women of different ethnicities from eastern siberia |
| topic | hyperandrogenism androgens LC-MS/MS testosterone DHEAS cut-off |
| url | https://www.mdpi.com/2075-4418/13/1/33 |
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