Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers
Soybean meal (SBM) contains several anti-nutritional factors, which limit the use of soy protein. Exogenous enzyme supplementation and microbial fermentation can reduce anti-nutritional factors and increase the bioavailability of nutrients in SBM. In this study, we investigated the optimal parameter...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2019.1637344 |
| _version_ | 1828241192693792768 |
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| author | Yeong-Hsiang Cheng Felix Shih-Hsiang Hsiao Chiu-Ming Wen Chia-Ying Wu Andrzej Dybus Yu-Hsiang Yu |
| author_facet | Yeong-Hsiang Cheng Felix Shih-Hsiang Hsiao Chiu-Ming Wen Chia-Ying Wu Andrzej Dybus Yu-Hsiang Yu |
| author_sort | Yeong-Hsiang Cheng |
| collection | DOAJ |
| description | Soybean meal (SBM) contains several anti-nutritional factors, which limit the use of soy protein. Exogenous enzyme supplementation and microbial fermentation can reduce anti-nutritional factors and increase the bioavailability of nutrients in SBM. In this study, we investigated the optimal parameters for the solid-state fermentation (SSF) of SBM by protease in combination with probiotics and evaluated its effect on the growth performance and immune response in broilers. Protease in combination with Bacillus subtilis significantly increased the degradation of soybean protein and soybean allergens in a dose-dependent manner during the SSF of soybean meal. Broilers fed 10% fermented SBM (FSBM), which was produced with protease and B. subtilis, consumed more daily feed on average during the entire feeding period than the SBM group did (P < 0.05). Dietary supplementation with 10% FSBM attenuated IL-4 mRNA expression in the spleen and bursa of Fabricius of broilers (P < 0.05). Furthermore, 10%-FSBM-fed broilers had significantly lower serum anti-soybean IgG levels than SBM-fed broilers (P < 0.05). These results suggest that the supplementation of B. subtilis and protease during the SSF of SBM reduces anti-nutritional factors. Moreover, dietary supplementation of 10% FSBM could inhibit the allergic immune response in broilers. |
| first_indexed | 2024-04-12T21:54:50Z |
| format | Article |
| id | doaj.art-339679a08022412aa529adf7f46a70fd |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-04-12T21:54:50Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-339679a08022412aa529adf7f46a70fd2022-12-22T03:15:21ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442019-01-0147133934810.1080/09712119.2019.16373441637344Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilersYeong-Hsiang Cheng0Felix Shih-Hsiang Hsiao1Chiu-Ming Wen2Chia-Ying Wu3Andrzej Dybus4Yu-Hsiang Yu5Department of Biotechnology and Animal Science, National Ilan UniversityDepartment of Animal Science and Biotechnology, Tunghai UniversityDepartment of Life Sciences, National University of KaohsiungDepartment of Biotechnology and Animal Science, National Ilan UniversityLaboratory of Molecular Cytogenetics, Department of Ruminants Science, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of TechnologyDepartment of Biotechnology and Animal Science, National Ilan UniversitySoybean meal (SBM) contains several anti-nutritional factors, which limit the use of soy protein. Exogenous enzyme supplementation and microbial fermentation can reduce anti-nutritional factors and increase the bioavailability of nutrients in SBM. In this study, we investigated the optimal parameters for the solid-state fermentation (SSF) of SBM by protease in combination with probiotics and evaluated its effect on the growth performance and immune response in broilers. Protease in combination with Bacillus subtilis significantly increased the degradation of soybean protein and soybean allergens in a dose-dependent manner during the SSF of soybean meal. Broilers fed 10% fermented SBM (FSBM), which was produced with protease and B. subtilis, consumed more daily feed on average during the entire feeding period than the SBM group did (P < 0.05). Dietary supplementation with 10% FSBM attenuated IL-4 mRNA expression in the spleen and bursa of Fabricius of broilers (P < 0.05). Furthermore, 10%-FSBM-fed broilers had significantly lower serum anti-soybean IgG levels than SBM-fed broilers (P < 0.05). These results suggest that the supplementation of B. subtilis and protease during the SSF of SBM reduces anti-nutritional factors. Moreover, dietary supplementation of 10% FSBM could inhibit the allergic immune response in broilers.http://dx.doi.org/10.1080/09712119.2019.1637344bacillus subtilisbroilerproteasefermented soybean mealimmune response |
| spellingShingle | Yeong-Hsiang Cheng Felix Shih-Hsiang Hsiao Chiu-Ming Wen Chia-Ying Wu Andrzej Dybus Yu-Hsiang Yu Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers Journal of Applied Animal Research bacillus subtilis broiler protease fermented soybean meal immune response |
| title | Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers |
| title_full | Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers |
| title_fullStr | Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers |
| title_full_unstemmed | Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers |
| title_short | Mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers |
| title_sort | mixed fermentation of soybean meal by protease and probiotics and its effects on the growth performance and immune response in broilers |
| topic | bacillus subtilis broiler protease fermented soybean meal immune response |
| url | http://dx.doi.org/10.1080/09712119.2019.1637344 |
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