Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge
<p>Abstract</p> <p>Time-resolved quantitative colocalization analysis is a method based on confocal fluorescence microscopy allowing for a sophisticated characterization of nanomaterials with respect to their intracellular trafficking. This technique was applied to relate the inter...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://www.jnanobiotechnology.com/content/10/1/28 |
| _version_ | 1811184820589428736 |
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| author | Schweiger Christoph Hartmann Raimo Zhang Feng Parak Wolfgang J Kissel Thomas H Rivera_Gil Pilar |
| author_facet | Schweiger Christoph Hartmann Raimo Zhang Feng Parak Wolfgang J Kissel Thomas H Rivera_Gil Pilar |
| author_sort | Schweiger Christoph |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Time-resolved quantitative colocalization analysis is a method based on confocal fluorescence microscopy allowing for a sophisticated characterization of nanomaterials with respect to their intracellular trafficking. This technique was applied to relate the internalization patterns of nanoparticles <it>i</it>.<it>e</it>. superparamagnetic iron oxide nanoparticles with distinct physicochemical characteristics with their uptake mechanism, rate and intracellular fate.</p> <p>The physicochemical characterization of the nanoparticles showed particles of approximately the same size and shape as well as similar magnetic properties, only differing in charge due to different surface coatings. Incubation of the cells with both nanoparticles resulted in strong differences in the internalization rate and in the intracellular localization depending on the charge. Quantitative and qualitative analysis of nanoparticles-organelle colocalization experiments revealed that positively charged particles were found to enter the cells faster using different endocytotic pathways than their negative counterparts. Nevertheless, both nanoparticles species were finally enriched inside lysosomal structures and their efficiency in agarose phantom relaxometry experiments was very similar.</p> <p>This quantitative analysis demonstrates that charge is a key factor influencing the nanoparticle-cell interactions, specially their intracellular accumulation. Despite differences in their physicochemical properties and intracellular distribution, the efficiencies of both nanoparticles as MRI agents were not significantly different.</p> |
| first_indexed | 2024-04-11T13:20:00Z |
| format | Article |
| id | doaj.art-3397c2ff779f4b37aa9a5a6c32248622 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T13:20:00Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-3397c2ff779f4b37aa9a5a6c322486222022-12-22T04:22:15ZengBMCJournal of Nanobiotechnology1477-31552012-07-011012810.1186/1477-3155-10-28Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite chargeSchweiger ChristophHartmann RaimoZhang FengParak Wolfgang JKissel Thomas HRivera_Gil Pilar<p>Abstract</p> <p>Time-resolved quantitative colocalization analysis is a method based on confocal fluorescence microscopy allowing for a sophisticated characterization of nanomaterials with respect to their intracellular trafficking. This technique was applied to relate the internalization patterns of nanoparticles <it>i</it>.<it>e</it>. superparamagnetic iron oxide nanoparticles with distinct physicochemical characteristics with their uptake mechanism, rate and intracellular fate.</p> <p>The physicochemical characterization of the nanoparticles showed particles of approximately the same size and shape as well as similar magnetic properties, only differing in charge due to different surface coatings. Incubation of the cells with both nanoparticles resulted in strong differences in the internalization rate and in the intracellular localization depending on the charge. Quantitative and qualitative analysis of nanoparticles-organelle colocalization experiments revealed that positively charged particles were found to enter the cells faster using different endocytotic pathways than their negative counterparts. Nevertheless, both nanoparticles species were finally enriched inside lysosomal structures and their efficiency in agarose phantom relaxometry experiments was very similar.</p> <p>This quantitative analysis demonstrates that charge is a key factor influencing the nanoparticle-cell interactions, specially their intracellular accumulation. Despite differences in their physicochemical properties and intracellular distribution, the efficiencies of both nanoparticles as MRI agents were not significantly different.</p>http://www.jnanobiotechnology.com/content/10/1/28Superparamagnetic iron oxide nanoparticles (SPIONs)Intracellular distributionChargeCoatingSizeQuantitative correlation analysisColocalization |
| spellingShingle | Schweiger Christoph Hartmann Raimo Zhang Feng Parak Wolfgang J Kissel Thomas H Rivera_Gil Pilar Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge Journal of Nanobiotechnology Superparamagnetic iron oxide nanoparticles (SPIONs) Intracellular distribution Charge Coating Size Quantitative correlation analysis Colocalization |
| title | Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge |
| title_full | Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge |
| title_fullStr | Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge |
| title_full_unstemmed | Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge |
| title_short | Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge |
| title_sort | quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge |
| topic | Superparamagnetic iron oxide nanoparticles (SPIONs) Intracellular distribution Charge Coating Size Quantitative correlation analysis Colocalization |
| url | http://www.jnanobiotechnology.com/content/10/1/28 |
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