| Summary: | MOMAST<sup>(®)</sup> HY100 and MOMAST<sup>(®)</sup> HP30 are polyphenolic liquid complexes from olive pressing juice with a total polyphenolic content of 100 g/kg (at least 50% as hydroxytyrosol) and 36 g/kg (at least 30% as hydroxytyrosol), respectively. We investigated the potential protective role of MOMAST<sup>(®)</sup> HY100 and MOMAST<sup>(®)</sup> HP30 on isolated rat colon, liver, heart, and prefrontal cortex specimens treated with <i>Escherichia coli</i> lipopolysaccharide (LPS), a validated ex vivo model of inflammation, by measuring the production of prostaglandin (PG)E<sub>2</sub>, 8-iso-PGF<sub>2α</sub>, lactate dehydrogenase (LDH), as well as cyclooxygenase (COX)-2, tumor necrosis factor α (TNFα), and inducible nitric oxide synthase (iNOS) mRNA levels. MOMAST<sup>(®)</sup> HY100 decreased LPS-stimulated PGE<sub>2</sub> and LDH levels in all tested tissues. Following treatment with MOMAST<sup>(®)</sup> HY100, we found a significant reduction in iNOS levels in prefrontal cortex and heart specimens, COX-2 and TNFα mRNA levels in heart specimens, and 8-iso-PGF<sub>2α</sub> levels in liver specimens. On the other hand, MOMAST<sup>(®)</sup> HP30 was found to blunt COX-2, TNFα, and iNOS mRNA levels, as well as 8-iso-PGF<sub>2α</sub> in cortex, liver, and colon specimens. MOMAST<sup>(®)</sup> HP30 was also found to decrease PGE<sub>2</sub> levels in liver specimens, while it decreased iNOS mRNA, LDH, and 8-iso-PGF<sub>2α</sub> levels in heart specimens. Both MOMAST<sup>(®)</sup> HY100 and MOMAST<sup>(®)</sup> HP30 exhibited protective effects on multiple inflammatory and oxidative stress pathways.
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