Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum
Abstract Background Mycoplasma synoviae (MS) is known to cause Eggshell Apex Abnormality (EAA) syndrome characterized by an altered shell surface with increased translucency on the apex. However, no large-scale studies have been conducted to obtain prevalence data of EAA and MS isolates associated t...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-08-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-020-02487-0 |
| _version_ | 1818148074713776128 |
|---|---|
| author | M. Cisneros-Tamayo I. Kempf J. Coton V. Michel S. Bougeard C. de Boisséson P. Lucas M.-H. Bäyon-Auboyer G. Chiron C. Mindus A. V. Gautier-Bouchardon |
| author_facet | M. Cisneros-Tamayo I. Kempf J. Coton V. Michel S. Bougeard C. de Boisséson P. Lucas M.-H. Bäyon-Auboyer G. Chiron C. Mindus A. V. Gautier-Bouchardon |
| author_sort | M. Cisneros-Tamayo |
| collection | DOAJ |
| description | Abstract Background Mycoplasma synoviae (MS) is known to cause Eggshell Apex Abnormality (EAA) syndrome characterized by an altered shell surface with increased translucency on the apex. However, no large-scale studies have been conducted to obtain prevalence data of EAA and MS isolates associated to this syndrome. This manuscript reports the results of two field studies performed in the French poultry industry (2015–2017): focusing mainly on investigation of presence and prevalence of EAA in different types of laying hen flocks (phase 1), and isolation of MS strains from EAA-infected flocks (phase 2). Results The first survey included 77 farms of commercial layers in three French egg-production regions, hosting 40 flocks in alternative systems (ALT) and 56 in furnished cages (FC). Seven flocks (4 FC and 3 ALT) presented EAA clinical signs, giving a prevalence of 7.3% in this studied sample. A second independent field study was conducted to identify MS by in vitro cultivation and PCR in samples from 28 flocks with clinical signs of EAA. Different types of biological specimens were collected in EAA-affected flocks and submitted to the laboratory. M. synoviae was detected in 25/28 flocks, from both production systems (5/5 ALT and 20/23 FC). Detection of MS was significantly higher in tracheal swabs (59%) than in cloacal (10.5%), albumen (3.6%) and egg yolk (1.1%) swabs. It is worth to mention that attempts to clone MS from positive samples were often hampered by the presence of another Mycoplasma species, which showed fast growing behaviour in the selective media used in this study (Frey Medium 4 and Frey Medium 4 supplemented with erythromycin). The use of MALDI-TOF mass spectrometry in combination with next-generation sequencing (NGS) results allowed the identification of this fast growing mycoplasma as Mycoplasma pullorum, which was detected in 14 of the 25 (56%) MS-positive flocks. Conclusions These results confirmed the presence of the EAA syndrome in MS-positive flocks of layers in France, reared in different regions and in different production systems (ALT and FC). Studies need to be conducted to test whether M. pullorum may influence the expression of clinical signs of EAA in MS-infected layer farms. |
| first_indexed | 2024-12-11T12:45:22Z |
| format | Article |
| id | doaj.art-339a8d90ad464511a9b0b1156fc2c2c4 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-11T12:45:22Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-339a8d90ad464511a9b0b1156fc2c2c42022-12-22T01:06:50ZengBMCBMC Veterinary Research1746-61482020-08-0116111310.1186/s12917-020-02487-0Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorumM. Cisneros-Tamayo0I. Kempf1J. Coton2V. Michel3S. Bougeard4C. de Boisséson5P. Lucas6M.-H. Bäyon-Auboyer7G. Chiron8C. Mindus9A. V. Gautier-Bouchardon10Mycoplasmology, Bacteriology and Antimicrobial Resistance Unit, Ploufragan-Plouzané-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES)Mycoplasmology, Bacteriology and Antimicrobial Resistance Unit, Ploufragan-Plouzané-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES)Epidemiology and Welfare in Poultry and Rabbits, Ploufragan-Plouzané-Niort Laboratory, ANSESAnimal Welfare National Coordination, ANSESEpidemiology and Welfare in Pigs, Ploufragan-Plouzané-Niort Laboratory, ANSESViral Genetics and Biosafety unit, Ploufragan-Plouzané-Niort Laboratory, ANSESViral Genetics and Biosafety unit, Ploufragan-Plouzané-Niort Laboratory, ANSESLaboceaITAVIITAVIMycoplasmology, Bacteriology and Antimicrobial Resistance Unit, Ploufragan-Plouzané-Niort Laboratory, French Agency for Food, Environmental and Occupational Health and Safety (ANSES)Abstract Background Mycoplasma synoviae (MS) is known to cause Eggshell Apex Abnormality (EAA) syndrome characterized by an altered shell surface with increased translucency on the apex. However, no large-scale studies have been conducted to obtain prevalence data of EAA and MS isolates associated to this syndrome. This manuscript reports the results of two field studies performed in the French poultry industry (2015–2017): focusing mainly on investigation of presence and prevalence of EAA in different types of laying hen flocks (phase 1), and isolation of MS strains from EAA-infected flocks (phase 2). Results The first survey included 77 farms of commercial layers in three French egg-production regions, hosting 40 flocks in alternative systems (ALT) and 56 in furnished cages (FC). Seven flocks (4 FC and 3 ALT) presented EAA clinical signs, giving a prevalence of 7.3% in this studied sample. A second independent field study was conducted to identify MS by in vitro cultivation and PCR in samples from 28 flocks with clinical signs of EAA. Different types of biological specimens were collected in EAA-affected flocks and submitted to the laboratory. M. synoviae was detected in 25/28 flocks, from both production systems (5/5 ALT and 20/23 FC). Detection of MS was significantly higher in tracheal swabs (59%) than in cloacal (10.5%), albumen (3.6%) and egg yolk (1.1%) swabs. It is worth to mention that attempts to clone MS from positive samples were often hampered by the presence of another Mycoplasma species, which showed fast growing behaviour in the selective media used in this study (Frey Medium 4 and Frey Medium 4 supplemented with erythromycin). The use of MALDI-TOF mass spectrometry in combination with next-generation sequencing (NGS) results allowed the identification of this fast growing mycoplasma as Mycoplasma pullorum, which was detected in 14 of the 25 (56%) MS-positive flocks. Conclusions These results confirmed the presence of the EAA syndrome in MS-positive flocks of layers in France, reared in different regions and in different production systems (ALT and FC). Studies need to be conducted to test whether M. pullorum may influence the expression of clinical signs of EAA in MS-infected layer farms.http://link.springer.com/article/10.1186/s12917-020-02487-0Mycoplasma synoviaeEggshell apex abnormalitiesLayersMALDI-TOFMycoplasma pullorum |
| spellingShingle | M. Cisneros-Tamayo I. Kempf J. Coton V. Michel S. Bougeard C. de Boisséson P. Lucas M.-H. Bäyon-Auboyer G. Chiron C. Mindus A. V. Gautier-Bouchardon Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum BMC Veterinary Research Mycoplasma synoviae Eggshell apex abnormalities Layers MALDI-TOF Mycoplasma pullorum |
| title | Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum |
| title_full | Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum |
| title_fullStr | Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum |
| title_full_unstemmed | Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum |
| title_short | Investigation on eggshell apex abnormality (EAA) syndrome in France: isolation of Mycoplasma synoviae is frequently associated with Mycoplasma pullorum |
| title_sort | investigation on eggshell apex abnormality eaa syndrome in france isolation of mycoplasma synoviae is frequently associated with mycoplasma pullorum |
| topic | Mycoplasma synoviae Eggshell apex abnormalities Layers MALDI-TOF Mycoplasma pullorum |
| url | http://link.springer.com/article/10.1186/s12917-020-02487-0 |
| work_keys_str_mv | AT mcisnerostamayo investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT ikempf investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT jcoton investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT vmichel investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT sbougeard investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT cdeboisseson investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT plucas investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT mhbayonauboyer investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT gchiron investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT cmindus investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum AT avgautierbouchardon investigationoneggshellapexabnormalityeaasyndromeinfranceisolationofmycoplasmasynoviaeisfrequentlyassociatedwithmycoplasmapullorum |