Assessment of microRNA profile of kidney biopsies of patients with lupus nephritis
Background: The changes in some epigenetic elements such as microRNAs result in aberrant immune responses leading to production and secretion of nephritogenic autoantibodies as the main fundament of lupus nephritis (LN). Objectives: The present study aimed to assess the miRNA profile of kidney biops...
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| Format: | Article |
| Language: | English |
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Society of Diabetic Nephropathy Prevention
2017-10-01
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| Series: | Journal of Nephropathology |
| Subjects: | |
| Online Access: | https://nephropathol.com/PDF/jnp-6-333.pdf |
| _version_ | 1797827965109665792 |
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| author | Tahereh Malakoutian Sepideh Hajian Ahmad Ebrahimi Koosha Kamali |
| author_facet | Tahereh Malakoutian Sepideh Hajian Ahmad Ebrahimi Koosha Kamali |
| author_sort | Tahereh Malakoutian |
| collection | DOAJ |
| description | Background: The changes in some epigenetic elements such as microRNAs result in aberrant immune responses leading to production and secretion of nephritogenic autoantibodies as the main fundament of lupus nephritis (LN). Objectives: The present study aimed to assess the miRNA profile of kidney biopsies in patients with LN with the purpose of describing the critical role of these elements in LN creation. Patients and Methods: In this case-control single center study 11 patients who suffered LN (as the case group) and 11 patients with normal kidney function who were candidate for nephrectomy due to cancer or cyst (as the control group) were included. Kidney biopsies were taken from all LN and control subjects. RNA was extracted and converted to cDNA, then the cDNA was evaluated using NANODROP and then intra-renal expression of candidate miRNAs were quantified in the two groups. In the present study, four topranked miRNAs, miR-638, miR-146a, miR-198, and miR-731 were selected for qRT-PCR. Results: Consistent with the microarray data, we found no significant difference in the expression of all miRNAs between LN and control groups. Using REST 2009 software, we did not also reveal any difference in expression of four miRNAs studied between the patients with LN and those without LN in both parametric and nonparametric patterns. Conclusions: The expression of miR-638, miR-146a, miR-198, and miR-731 may not be related to occurrence of LN in Iranian population. |
| first_indexed | 2024-04-09T12:56:52Z |
| format | Article |
| id | doaj.art-33a127a2c1ac4719947553eaabc24bea |
| institution | Directory Open Access Journal |
| issn | 2251-8363 2251-8819 |
| language | English |
| last_indexed | 2024-04-09T12:56:52Z |
| publishDate | 2017-10-01 |
| publisher | Society of Diabetic Nephropathy Prevention |
| record_format | Article |
| series | Journal of Nephropathology |
| spelling | doaj.art-33a127a2c1ac4719947553eaabc24bea2023-05-13T11:31:11ZengSociety of Diabetic Nephropathy PreventionJournal of Nephropathology2251-83632251-88192017-10-016433333710.15171/npj.2017.53jnp-20170802162941Assessment of microRNA profile of kidney biopsies of patients with lupus nephritisTahereh Malakoutian0Sepideh Hajian1Ahmad Ebrahimi2Koosha Kamali3Department of Nephrology, Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, IranDepartment of Nephrology, Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, IranDepartment of Genetics, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Urology, Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, IranBackground: The changes in some epigenetic elements such as microRNAs result in aberrant immune responses leading to production and secretion of nephritogenic autoantibodies as the main fundament of lupus nephritis (LN). Objectives: The present study aimed to assess the miRNA profile of kidney biopsies in patients with LN with the purpose of describing the critical role of these elements in LN creation. Patients and Methods: In this case-control single center study 11 patients who suffered LN (as the case group) and 11 patients with normal kidney function who were candidate for nephrectomy due to cancer or cyst (as the control group) were included. Kidney biopsies were taken from all LN and control subjects. RNA was extracted and converted to cDNA, then the cDNA was evaluated using NANODROP and then intra-renal expression of candidate miRNAs were quantified in the two groups. In the present study, four topranked miRNAs, miR-638, miR-146a, miR-198, and miR-731 were selected for qRT-PCR. Results: Consistent with the microarray data, we found no significant difference in the expression of all miRNAs between LN and control groups. Using REST 2009 software, we did not also reveal any difference in expression of four miRNAs studied between the patients with LN and those without LN in both parametric and nonparametric patterns. Conclusions: The expression of miR-638, miR-146a, miR-198, and miR-731 may not be related to occurrence of LN in Iranian population.https://nephropathol.com/PDF/jnp-6-333.pdfslekidneybiopsymicrornalupus nephritis |
| spellingShingle | Tahereh Malakoutian Sepideh Hajian Ahmad Ebrahimi Koosha Kamali Assessment of microRNA profile of kidney biopsies of patients with lupus nephritis Journal of Nephropathology sle kidney biopsy microrna lupus nephritis |
| title | Assessment of microRNA profile of kidney biopsies of patients with lupus nephritis |
| title_full | Assessment of microRNA profile of kidney biopsies of patients with lupus nephritis |
| title_fullStr | Assessment of microRNA profile of kidney biopsies of patients with lupus nephritis |
| title_full_unstemmed | Assessment of microRNA profile of kidney biopsies of patients with lupus nephritis |
| title_short | Assessment of microRNA profile of kidney biopsies of patients with lupus nephritis |
| title_sort | assessment of microrna profile of kidney biopsies of patients with lupus nephritis |
| topic | sle kidney biopsy microrna lupus nephritis |
| url | https://nephropathol.com/PDF/jnp-6-333.pdf |
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