Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused Medicines
Progress in macrophage research is crucial for numerous applications in medicine, including cancer and infectious diseases. However, the existing methods to manipulate living macrophages are labor-intense and inconvenient. Here, we show that macrophage membranes can be reconstituted after storage fo...
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/10/2769 |
| _version_ | 1827721570578071552 |
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| author | Igor D. Zlotnikov Elena V. Kudryashova |
| author_facet | Igor D. Zlotnikov Elena V. Kudryashova |
| author_sort | Igor D. Zlotnikov |
| collection | DOAJ |
| description | Progress in macrophage research is crucial for numerous applications in medicine, including cancer and infectious diseases. However, the existing methods to manipulate living macrophages are labor-intense and inconvenient. Here, we show that macrophage membranes can be reconstituted after storage for months at 4 °C, with their CD206 receptor selectivity and specificity being similar to those in the living cells. Then, we have developed a mannose ligand, specific to CD206, linked with PEG as an IR spectroscopy marker to detect binding with the macrophage receptor. PEG was selected due to its unique adsorption band of the C–O–C group at IR spectra, which does not overlap with other biomolecules’ spectroscopic feature. Next, competitive binding assay versus the PEG-bound ligand has enabled the selection of other higher-affinity ligands specific to CD206. Furthermore, those higher-affinity ligands were used to differentiate activated macrophages in a patient’s bronchoalveolar (BAL) or nasopharyngeal (NPL) lavage. CD206− control cells (HEK293T) showed only non-specific binding. Therefore, biochips based on reconstituted macrophage membranes as well as PEG-trimannoside as an IR spectroscopic marker can be used to develop new methods facilitating macrophage research and macrophage-focused drug discovery. |
| first_indexed | 2024-03-10T21:24:15Z |
| format | Article |
| id | doaj.art-33a1395dced74358a322d7b2416d15bd |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T21:24:15Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-33a1395dced74358a322d7b2416d15bd2023-11-19T15:46:50ZengMDPI AGBiomedicines2227-90592023-10-011110276910.3390/biomedicines11102769Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused MedicinesIgor D. Zlotnikov0Elena V. Kudryashova1Faculty of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, RussiaProgress in macrophage research is crucial for numerous applications in medicine, including cancer and infectious diseases. However, the existing methods to manipulate living macrophages are labor-intense and inconvenient. Here, we show that macrophage membranes can be reconstituted after storage for months at 4 °C, with their CD206 receptor selectivity and specificity being similar to those in the living cells. Then, we have developed a mannose ligand, specific to CD206, linked with PEG as an IR spectroscopy marker to detect binding with the macrophage receptor. PEG was selected due to its unique adsorption band of the C–O–C group at IR spectra, which does not overlap with other biomolecules’ spectroscopic feature. Next, competitive binding assay versus the PEG-bound ligand has enabled the selection of other higher-affinity ligands specific to CD206. Furthermore, those higher-affinity ligands were used to differentiate activated macrophages in a patient’s bronchoalveolar (BAL) or nasopharyngeal (NPL) lavage. CD206− control cells (HEK293T) showed only non-specific binding. Therefore, biochips based on reconstituted macrophage membranes as well as PEG-trimannoside as an IR spectroscopic marker can be used to develop new methods facilitating macrophage research and macrophage-focused drug discovery.https://www.mdpi.com/2227-9059/11/10/2769macrophage-based biochipCD206bronchoalveolar lavagediagnostics |
| spellingShingle | Igor D. Zlotnikov Elena V. Kudryashova Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused Medicines Biomedicines macrophage-based biochip CD206 bronchoalveolar lavage diagnostics |
| title | Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused Medicines |
| title_full | Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused Medicines |
| title_fullStr | Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused Medicines |
| title_full_unstemmed | Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused Medicines |
| title_short | Biomimetic System Based on Reconstituted Macrophage Membranes for Analyzing and Selection of Higher-Affinity Ligands Specific to Mannose Receptor to Develop the Macrophage-Focused Medicines |
| title_sort | biomimetic system based on reconstituted macrophage membranes for analyzing and selection of higher affinity ligands specific to mannose receptor to develop the macrophage focused medicines |
| topic | macrophage-based biochip CD206 bronchoalveolar lavage diagnostics |
| url | https://www.mdpi.com/2227-9059/11/10/2769 |
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