Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit.
Enduring reorganization is accepted as a fundamental process of adult neural plasticity. The most dramatic example of this reorganization is the birth and continuously occurring incorporation of new neurons into the pre-existing network of the adult mammalian hippocampus. Based on this phenomenon we...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3688776?pdf=render |
| _version_ | 1819019184035594240 |
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| author | Franziska Neuser Martin Polack Christine Annaheim Kerry L Tucker Martin Korte |
| author_facet | Franziska Neuser Martin Polack Christine Annaheim Kerry L Tucker Martin Korte |
| author_sort | Franziska Neuser |
| collection | DOAJ |
| description | Enduring reorganization is accepted as a fundamental process of adult neural plasticity. The most dramatic example of this reorganization is the birth and continuously occurring incorporation of new neurons into the pre-existing network of the adult mammalian hippocampus. Based on this phenomenon we transplanted murine embryonic stem (ES)-cell derived neuronal precursors (ESNPs) into murine organotypic hippocampal slice cultures (OHC) and examined their integration. Using a precise quantitative morphological analysis combined with a detailed electrophysiology, we show a region-specific morphological integration of transplanted ESNPs into different subfields of the hippocampal tissue, resulting in pyramidal neuron-like embryonic stem cell-derived neurons (ESNs) in the Cornu Ammonis (CA1 and CA3) and granule neuron-like ESNs in the dentate gyrus (DG), respectively. Subregion specific structural maturation was accompanied by the development of dendritic spines and the generation of excitatory postsynaptic currents (EPSCs). This cell type specific development does not depend upon NMDA-receptor-dependent synaptic transmission. The presented integration approach was further used to determine the cell-autonomous function of the pan-neurotrophin receptor p75 (P75(NTR)), as a possible negative regulator of ESN integration. By this means we used p75(NTR)-deficient ESNPs to study their integration into a WT organotypic environment. We show here that p75(NTR) is not necessary for integration per se but plays a suppressing role in dendritic development. |
| first_indexed | 2024-12-21T03:31:16Z |
| format | Article |
| id | doaj.art-33a1f0bcd3414876aa1b3e453a56885b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T03:31:16Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-33a1f0bcd3414876aa1b3e453a56885b2022-12-21T19:17:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6649710.1371/journal.pone.0066497Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit.Franziska NeuserMartin PolackChristine AnnaheimKerry L TuckerMartin KorteEnduring reorganization is accepted as a fundamental process of adult neural plasticity. The most dramatic example of this reorganization is the birth and continuously occurring incorporation of new neurons into the pre-existing network of the adult mammalian hippocampus. Based on this phenomenon we transplanted murine embryonic stem (ES)-cell derived neuronal precursors (ESNPs) into murine organotypic hippocampal slice cultures (OHC) and examined their integration. Using a precise quantitative morphological analysis combined with a detailed electrophysiology, we show a region-specific morphological integration of transplanted ESNPs into different subfields of the hippocampal tissue, resulting in pyramidal neuron-like embryonic stem cell-derived neurons (ESNs) in the Cornu Ammonis (CA1 and CA3) and granule neuron-like ESNs in the dentate gyrus (DG), respectively. Subregion specific structural maturation was accompanied by the development of dendritic spines and the generation of excitatory postsynaptic currents (EPSCs). This cell type specific development does not depend upon NMDA-receptor-dependent synaptic transmission. The presented integration approach was further used to determine the cell-autonomous function of the pan-neurotrophin receptor p75 (P75(NTR)), as a possible negative regulator of ESN integration. By this means we used p75(NTR)-deficient ESNPs to study their integration into a WT organotypic environment. We show here that p75(NTR) is not necessary for integration per se but plays a suppressing role in dendritic development.http://europepmc.org/articles/PMC3688776?pdf=render |
| spellingShingle | Franziska Neuser Martin Polack Christine Annaheim Kerry L Tucker Martin Korte Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit. PLoS ONE |
| title | Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit. |
| title_full | Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit. |
| title_fullStr | Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit. |
| title_full_unstemmed | Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit. |
| title_short | Region-specific integration of embryonic stem cell-derived neuronal precursors into a pre-existing neuronal circuit. |
| title_sort | region specific integration of embryonic stem cell derived neuronal precursors into a pre existing neuronal circuit |
| url | http://europepmc.org/articles/PMC3688776?pdf=render |
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