Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profiling
<p>Abstract</p> <p>Background</p> <p>Plant Growth Promoting Rhizobacteria (PGPR), <it>Pseudomonas fluorescens </it>strain KH-1 was found to exhibit plant growth promotional activity in rice under both <it>in-vitro </it>and <it>in-vivo </...
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| Format: | Article |
| Language: | English |
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BMC
2009-12-01
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| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/7/1/47 |
| _version_ | 1811319029032288256 |
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| author | Thiruvengadam Raguchander Seetharaman Suresh Duraisamy Saravanakumar Muthuraj Raveendran Loganathan Karthiba Kandasamy Saveetha Ponnusamy Balasubramanian Ramasamy Samiyappan |
| author_facet | Thiruvengadam Raguchander Seetharaman Suresh Duraisamy Saravanakumar Muthuraj Raveendran Loganathan Karthiba Kandasamy Saveetha Ponnusamy Balasubramanian Ramasamy Samiyappan |
| author_sort | Thiruvengadam Raguchander |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Plant Growth Promoting Rhizobacteria (PGPR), <it>Pseudomonas fluorescens </it>strain KH-1 was found to exhibit plant growth promotional activity in rice under both <it>in-vitro </it>and <it>in-vivo </it>conditions. But the mechanism underlying such promotional activity of <it>P. fluorescens </it>is not yet understood clearly. In this study, efforts were made to elucidate the molecular responses of rice plants to <it>P. fluorescens </it>treatment through protein profiling. Two-dimensional polyacrylamide gel electrophoresis strategy was adopted to identify the PGPR responsive proteins and the differentially expressed proteins were analyzed by mass spectrometry.</p> <p>Results</p> <p>Priming of <it>P. fluorescens</it>, 23 different proteins found to be differentially expressed in rice leaf sheaths and MS analysis revealed the differential expression of some important proteins namely putative p23 co-chaperone, Thioredoxin h- rice, Ribulose-bisphosphate carboxylase large chain precursor, Nucleotide diPhosphate kinase, Proteosome sub unit protein and putative glutathione S-transferase protein.</p> <p>Conclusion</p> <p>Functional analyses of the differential proteins were reported to be directly or indirectly involved in growth promotion in plants. Thus, this study confirms the primary role of PGPR strain KH-1 in rice plant growth promotion.</p> |
| first_indexed | 2024-04-13T12:35:11Z |
| format | Article |
| id | doaj.art-33a3893c7669497da16edbe99b6eb645 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-04-13T12:35:11Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-33a3893c7669497da16edbe99b6eb6452022-12-22T02:46:40ZengBMCProteome Science1477-59562009-12-01714710.1186/1477-5956-7-47Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profilingThiruvengadam RaguchanderSeetharaman SureshDuraisamy SaravanakumarMuthuraj RaveendranLoganathan KarthibaKandasamy SaveethaPonnusamy BalasubramanianRamasamy Samiyappan<p>Abstract</p> <p>Background</p> <p>Plant Growth Promoting Rhizobacteria (PGPR), <it>Pseudomonas fluorescens </it>strain KH-1 was found to exhibit plant growth promotional activity in rice under both <it>in-vitro </it>and <it>in-vivo </it>conditions. But the mechanism underlying such promotional activity of <it>P. fluorescens </it>is not yet understood clearly. In this study, efforts were made to elucidate the molecular responses of rice plants to <it>P. fluorescens </it>treatment through protein profiling. Two-dimensional polyacrylamide gel electrophoresis strategy was adopted to identify the PGPR responsive proteins and the differentially expressed proteins were analyzed by mass spectrometry.</p> <p>Results</p> <p>Priming of <it>P. fluorescens</it>, 23 different proteins found to be differentially expressed in rice leaf sheaths and MS analysis revealed the differential expression of some important proteins namely putative p23 co-chaperone, Thioredoxin h- rice, Ribulose-bisphosphate carboxylase large chain precursor, Nucleotide diPhosphate kinase, Proteosome sub unit protein and putative glutathione S-transferase protein.</p> <p>Conclusion</p> <p>Functional analyses of the differential proteins were reported to be directly or indirectly involved in growth promotion in plants. Thus, this study confirms the primary role of PGPR strain KH-1 in rice plant growth promotion.</p>http://www.proteomesci.com/content/7/1/47 |
| spellingShingle | Thiruvengadam Raguchander Seetharaman Suresh Duraisamy Saravanakumar Muthuraj Raveendran Loganathan Karthiba Kandasamy Saveetha Ponnusamy Balasubramanian Ramasamy Samiyappan Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profiling Proteome Science |
| title | Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profiling |
| title_full | Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profiling |
| title_fullStr | Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profiling |
| title_full_unstemmed | Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profiling |
| title_short | Understanding the molecular basis of plant growth promotional effect of <it>Pseudomonas fluorescens </it>on rice through protein profiling |
| title_sort | understanding the molecular basis of plant growth promotional effect of it pseudomonas fluorescens it on rice through protein profiling |
| url | http://www.proteomesci.com/content/7/1/47 |
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