Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extract
Background: Scrophularia oblongifolia belongs to the Scrophulariaceae family, commonly known as the water figwort in Iran. It has been known as a traditional medicinal plant in Iran. Objective: This study was designed to investigate the phytochemicals and antimicrobial activity of ethyl acetate, met...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2023-03-01
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| Series: | Journal of Medicinal Plants |
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| Online Access: | http://jmp.ir/article-1-3454-en.pdf |
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| author | Masoumeh Sadeghi Nik Hosna Karimi Samad Nejad Ebrahimi Atousa Aliahmadi |
| author_facet | Masoumeh Sadeghi Nik Hosna Karimi Samad Nejad Ebrahimi Atousa Aliahmadi |
| author_sort | Masoumeh Sadeghi Nik |
| collection | DOAJ |
| description | Background: Scrophularia oblongifolia belongs to the Scrophulariaceae family, commonly known as the water figwort in Iran. It has been known as a traditional medicinal plant in Iran. Objective: This study was designed to investigate the phytochemicals and antimicrobial activity of ethyl acetate, methanol, MeOH: H2O (50:50 v/v), and n-hexane extract of Scrophularia oblongifolia aerial parts, growing in the Kangavar region in the Kermanshah province of Iran. Methods: Compounds of ethyl acetate extract were isolated by chromatographic techniques (HPLC-PDA-MS/MS-Q-TOF) and their structures were elucidated by spectral methods (1D and 2D NMR). The antifungal and antibacterial activities of some new compounds and ethyl acetate, methanol, and n-hexane extract were evaluated. Results: Two new polyphenol iridoid glycosides with phenylpropanoid moiety namely scrophuloside A5 and scrophuloside A6; two known phenylpropanoid glycosides namely scrophuloside A3 and scrophuloside A4, and two known iridoid glycosides namely aucubin and harpagoside were isolated from S. oblongifolia. Pure compounds showed high antifungal activities with MIC values ranging from 0.35 to 0.74 µg/ml against C. albicans and high antibacterial activities with MIC values ranging from 0.70 to 1.48 µg/ml against G. vajinalis. n-Hexan extract showed moderate antibacterial and antifungal activity, while methanol and methanol:H2O (50:50 v/v) extract showed no antimicrobial activities. Conclusion: Based on these findings, compounds isolated from S. oblongifolia have demonstrated potential antibacterial and antifungal activities. |
| first_indexed | 2024-03-12T22:00:50Z |
| format | Article |
| id | doaj.art-33a55affc25f413481aed8a63254eb71 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-03-12T22:00:50Z |
| publishDate | 2023-03-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-33a55affc25f413481aed8a63254eb712023-07-25T07:23:11ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582023-03-0122852544Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extractMasoumeh Sadeghi Nik0Hosna Karimi1Samad Nejad Ebrahimi2Atousa Aliahmadi3 Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran Background: Scrophularia oblongifolia belongs to the Scrophulariaceae family, commonly known as the water figwort in Iran. It has been known as a traditional medicinal plant in Iran. Objective: This study was designed to investigate the phytochemicals and antimicrobial activity of ethyl acetate, methanol, MeOH: H2O (50:50 v/v), and n-hexane extract of Scrophularia oblongifolia aerial parts, growing in the Kangavar region in the Kermanshah province of Iran. Methods: Compounds of ethyl acetate extract were isolated by chromatographic techniques (HPLC-PDA-MS/MS-Q-TOF) and their structures were elucidated by spectral methods (1D and 2D NMR). The antifungal and antibacterial activities of some new compounds and ethyl acetate, methanol, and n-hexane extract were evaluated. Results: Two new polyphenol iridoid glycosides with phenylpropanoid moiety namely scrophuloside A5 and scrophuloside A6; two known phenylpropanoid glycosides namely scrophuloside A3 and scrophuloside A4, and two known iridoid glycosides namely aucubin and harpagoside were isolated from S. oblongifolia. Pure compounds showed high antifungal activities with MIC values ranging from 0.35 to 0.74 µg/ml against C. albicans and high antibacterial activities with MIC values ranging from 0.70 to 1.48 µg/ml against G. vajinalis. n-Hexan extract showed moderate antibacterial and antifungal activity, while methanol and methanol:H2O (50:50 v/v) extract showed no antimicrobial activities. Conclusion: Based on these findings, compounds isolated from S. oblongifolia have demonstrated potential antibacterial and antifungal activities.http://jmp.ir/article-1-3454-en.pdfscrophularia oblongifoliascrophularia umbrosabiological activityantimicrobialwater figwortiridoid glycosides |
| spellingShingle | Masoumeh Sadeghi Nik Hosna Karimi Samad Nejad Ebrahimi Atousa Aliahmadi Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extract Journal of Medicinal Plants scrophularia oblongifolia scrophularia umbrosa biological activity antimicrobial water figwort iridoid glycosides |
| title | Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extract |
| title_full | Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extract |
| title_fullStr | Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extract |
| title_full_unstemmed | Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extract |
| title_short | Phytochemical analysis and antimicrobial activities of Scrophularia oblongifolia Loisel. ethyl acetate extract |
| title_sort | phytochemical analysis and antimicrobial activities of scrophularia oblongifolia loisel ethyl acetate extract |
| topic | scrophularia oblongifolia scrophularia umbrosa biological activity antimicrobial water figwort iridoid glycosides |
| url | http://jmp.ir/article-1-3454-en.pdf |
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