Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment
The aim of this study is to develop doxycycline imprinted contact lenses that will be used in the treatment of corneal neovascularization, which can eventually cause blindness. For this purpose, doxycycline imprinted contact lenses were first prepared, and then they were loaded with doxycycline and...
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| Format: | Article |
| Language: | English |
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Turkish Chemical Society
2018-10-01
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| Series: | Journal of the Turkish Chemical Society, Section A: Chemistry |
| Subjects: | |
| Online Access: | http://dergipark.gov.tr/jotcsa/issue/37193/428846 |
| _version_ | 1797922648587501568 |
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| author | Sibel Erkal Ilhan Özge Kürkçüoğlu Tuğçe Inan Özde Zeynep Güner Dilek Dalgakıran Begüm Okutan Gamze Torun Köse Ayça Kırmızı Aylin Okçu Heper Özlem Gürses F. Seniha Güner |
| author_facet | Sibel Erkal Ilhan Özge Kürkçüoğlu Tuğçe Inan Özde Zeynep Güner Dilek Dalgakıran Begüm Okutan Gamze Torun Köse Ayça Kırmızı Aylin Okçu Heper Özlem Gürses F. Seniha Güner |
| author_sort | Sibel Erkal Ilhan |
| collection | DOAJ |
| description | The aim of this study is to develop doxycycline imprinted contact lenses that will be used in the treatment of corneal neovascularization, which can eventually cause blindness. For this purpose, doxycycline imprinted contact lenses were first prepared, and then they were loaded with doxycycline and their in vitro and in vivo performances were determined. In the synthesis of the contact lenses, 2-hydroxyethyl methacrylate was used as a backbone monomer. The functional monomer was selected as itaconic acid using molecular simulations. Doxycycline release profile of the lenses was determined in NaCl solution and their cytotoxic response was investigated on retinal pigment epithelium cells. In vivo experiments in rat models were performed to study the treatment patterns. The rats were sacrificed fifteen days after treatment, and clinical examination under optical microscope was performed to evaluate neovascularization, infiltration of inflammatory cells, and corneal epithelial changes. In conclusion; doxycycline imprinted contact lenses promise as an effective treatment method for corneal neovascularization. |
| first_indexed | 2024-04-10T14:34:46Z |
| format | Article |
| id | doaj.art-33a5aeb7ae994d41ab54741b64581ae9 |
| institution | Directory Open Access Journal |
| issn | 2149-0120 |
| language | English |
| last_indexed | 2024-04-10T14:34:46Z |
| publishDate | 2018-10-01 |
| publisher | Turkish Chemical Society |
| record_format | Article |
| series | Journal of the Turkish Chemical Society, Section A: Chemistry |
| spelling | doaj.art-33a5aeb7ae994d41ab54741b64581ae92023-02-15T16:08:38ZengTurkish Chemical SocietyJournal of the Turkish Chemical Society, Section A: Chemistry2149-01202018-10-01531185119210.18596/jotcsa.428846Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization TreatmentSibel Erkal Ilhan0Özge Kürkçüoğlu1Tuğçe Inan2 Özde Zeynep Güner3Dilek Dalgakıran4Begüm Okutan5Gamze Torun Köse6Ayça Kırmızı7 Aylin Okçu Heper8Özlem Gürses9 F. Seniha Güner10Ankara University, Faculty of Health Sciences, Midwifery Department, 06080 Altındağ Ankara, Turkeystanbul Technical University, Department of Chemical Engineering, Maslak 34469 Istanbul, Turkeystanbul Technical University, Department of Chemical Engineering, Maslak 34469 Istanbul, Turkeystanbul Technical University, Department of Chemical Engineering, Maslak 34469 Istanbul, TurkeyIstanbul Technical University, Polymer Science and Technology, Maslak 34469 Istanbul, TurkeyYeditepe University, Faculty of Engineering, Department Genetics and Bioengineering, Ataşehir 34755 Istanbul, TurkeyYeditepe University, Faculty of Engineering, Department Genetics and Bioengineering, Ataşehir 34755 Istanbul, TurkeyAnkara University, School of Medicine, Department of Medical Pathology, 06100 Sıhhıye Ankara, TurkeyAnkara University, School of Medicine, Department of Medical Pathology, 06100 Sıhhıye Ankara, TurkeyNew York Medical College, Department of Microbiology and Immunology, Valhalla, New York, USAstanbul Technical University, Department of Chemical Engineering, Maslak 34469 Istanbul, TurkeThe aim of this study is to develop doxycycline imprinted contact lenses that will be used in the treatment of corneal neovascularization, which can eventually cause blindness. For this purpose, doxycycline imprinted contact lenses were first prepared, and then they were loaded with doxycycline and their in vitro and in vivo performances were determined. In the synthesis of the contact lenses, 2-hydroxyethyl methacrylate was used as a backbone monomer. The functional monomer was selected as itaconic acid using molecular simulations. Doxycycline release profile of the lenses was determined in NaCl solution and their cytotoxic response was investigated on retinal pigment epithelium cells. In vivo experiments in rat models were performed to study the treatment patterns. The rats were sacrificed fifteen days after treatment, and clinical examination under optical microscope was performed to evaluate neovascularization, infiltration of inflammatory cells, and corneal epithelial changes. In conclusion; doxycycline imprinted contact lenses promise as an effective treatment method for corneal neovascularization.http://dergipark.gov.tr/jotcsa/issue/37193/428846Corneal neovascularizationContact lensDoxycycline |
| spellingShingle | Sibel Erkal Ilhan Özge Kürkçüoğlu Tuğçe Inan Özde Zeynep Güner Dilek Dalgakıran Begüm Okutan Gamze Torun Köse Ayça Kırmızı Aylin Okçu Heper Özlem Gürses F. Seniha Güner Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment Journal of the Turkish Chemical Society, Section A: Chemistry Corneal neovascularization Contact lens Doxycycline |
| title | Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment |
| title_full | Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment |
| title_fullStr | Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment |
| title_full_unstemmed | Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment |
| title_short | Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment |
| title_sort | preparation and determination of in vivo and in vitro performance of doxycycline imprinted contact lenses for corneal neovascularization treatment |
| topic | Corneal neovascularization Contact lens Doxycycline |
| url | http://dergipark.gov.tr/jotcsa/issue/37193/428846 |
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