Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach
Rhizome of Curcuma wenyujin, which is called EZhu in China, is a traditional Chinese medicine used to treat blood stasis for many years. However, the underlying mechanism of EZhu is not clear at present. In this study, plasma metabolomics combined with network pharmacology were used to elucidate the...
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MDPI AG
2018-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/24/1/82 |
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| author | Min Hao De Ji Lin Li Lianlin Su Wei Gu Liya Gu Qiaohan Wang Tulin Lu Chunqin Mao |
| author_facet | Min Hao De Ji Lin Li Lianlin Su Wei Gu Liya Gu Qiaohan Wang Tulin Lu Chunqin Mao |
| author_sort | Min Hao |
| collection | DOAJ |
| description | Rhizome of Curcuma wenyujin, which is called EZhu in China, is a traditional Chinese medicine used to treat blood stasis for many years. However, the underlying mechanism of EZhu is not clear at present. In this study, plasma metabolomics combined with network pharmacology were used to elucidate the therapeutic mechanism of EZhu in blood stasis from a metabolic perspective. The results showed that 26 potential metabolite markers of acute blood stasis were screened, and the levels were all reversed to different degrees by EZhu preadministration. Metabolic pathway analysis showed that the improvement of blood stasis by Curcuma wenyujin rhizome was mainly related to lipid metabolism (linoleic acid metabolism, ether lipid metabolism, sphingolipid metabolism, glycerophospholipid metabolism, and arachidonic acid metabolism) and amino acid metabolisms (tryptophan metabolism, lysine degradation). The component-target-pathway network showed that 68 target proteins were associated with 21 chemical components in EZhu. Five metabolic pathways of the network, including linoleic acid metabolism, sphingolipid metabolism, glycerolipid metabolism, arachidonic acid metabolism, and steroid hormone biosynthesis, were consistent with plasma metabolomics results. In conclusion, plasma metabolomics combined with network pharmacology can be helpful to clarify the mechanism of EZhu in improving blood stasis and to provide a literature basis for further research on the therapeutic mechanism of EZhu in clinical practice. |
| first_indexed | 2024-12-13T18:39:15Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T18:39:15Z |
| publishDate | 2018-12-01 |
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| spelling | doaj.art-33a6627c3306482182850e8d1b383d382022-12-21T23:35:16ZengMDPI AGMolecules1420-30492018-12-012418210.3390/molecules24010082molecules24010082Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network ApproachMin Hao0De Ji1Lin Li2Lianlin Su3Wei Gu4Liya Gu5Qiaohan Wang6Tulin Lu7Chunqin Mao8School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaRhizome of Curcuma wenyujin, which is called EZhu in China, is a traditional Chinese medicine used to treat blood stasis for many years. However, the underlying mechanism of EZhu is not clear at present. In this study, plasma metabolomics combined with network pharmacology were used to elucidate the therapeutic mechanism of EZhu in blood stasis from a metabolic perspective. The results showed that 26 potential metabolite markers of acute blood stasis were screened, and the levels were all reversed to different degrees by EZhu preadministration. Metabolic pathway analysis showed that the improvement of blood stasis by Curcuma wenyujin rhizome was mainly related to lipid metabolism (linoleic acid metabolism, ether lipid metabolism, sphingolipid metabolism, glycerophospholipid metabolism, and arachidonic acid metabolism) and amino acid metabolisms (tryptophan metabolism, lysine degradation). The component-target-pathway network showed that 68 target proteins were associated with 21 chemical components in EZhu. Five metabolic pathways of the network, including linoleic acid metabolism, sphingolipid metabolism, glycerolipid metabolism, arachidonic acid metabolism, and steroid hormone biosynthesis, were consistent with plasma metabolomics results. In conclusion, plasma metabolomics combined with network pharmacology can be helpful to clarify the mechanism of EZhu in improving blood stasis and to provide a literature basis for further research on the therapeutic mechanism of EZhu in clinical practice.http://www.mdpi.com/1420-3049/24/1/82Curcuma wenyujin rhizomeUPLC-Q/TOF-MSplasma metabolomicsmultivariate statistical analysisnetwork approach |
| spellingShingle | Min Hao De Ji Lin Li Lianlin Su Wei Gu Liya Gu Qiaohan Wang Tulin Lu Chunqin Mao Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach Molecules Curcuma wenyujin rhizome UPLC-Q/TOF-MS plasma metabolomics multivariate statistical analysis network approach |
| title | Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach |
| title_full | Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach |
| title_fullStr | Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach |
| title_full_unstemmed | Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach |
| title_short | Mechanism of Curcuma wenyujin Rhizoma on Acute Blood Stasis in Rats Based on a UPLC-Q/TOF-MS Metabolomics and Network Approach |
| title_sort | mechanism of curcuma wenyujin rhizoma on acute blood stasis in rats based on a uplc q tof ms metabolomics and network approach |
| topic | Curcuma wenyujin rhizome UPLC-Q/TOF-MS plasma metabolomics multivariate statistical analysis network approach |
| url | http://www.mdpi.com/1420-3049/24/1/82 |
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