Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening
Astrocytes are a major type of glial cell in the mammalian brain, essentially regulating neuronal development and function. Quantitative imaging represents an important approach to study astrocytic signaling in neural circuits. Focusing on astrocytic Ca2+ activity, a key pathway implicated in astroc...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | NeuroImage |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1053811920305553 |
| _version_ | 1819205570315419648 |
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| author | Cuong Pham Daniela Herrera Moro Christine Mouffle Steve Didienne Régine Hepp Frank W. Pfrieger Jean-Marie Mangin Pascal Legendre Claire Martin Serge Luquet Bruno Cauli Dongdong Li |
| author_facet | Cuong Pham Daniela Herrera Moro Christine Mouffle Steve Didienne Régine Hepp Frank W. Pfrieger Jean-Marie Mangin Pascal Legendre Claire Martin Serge Luquet Bruno Cauli Dongdong Li |
| author_sort | Cuong Pham |
| collection | DOAJ |
| description | Astrocytes are a major type of glial cell in the mammalian brain, essentially regulating neuronal development and function. Quantitative imaging represents an important approach to study astrocytic signaling in neural circuits. Focusing on astrocytic Ca2+ activity, a key pathway implicated in astrocye-neuron interaction, we here report a strategy combining fast light sheet fluorescence microscopy (LSFM) and correlative screening-based time series analysis, to map activity domains in astrocytes in living mammalian nerve tissue. Light sheet of micron-scale thickness enables wide-field optical sectioning to image astrocytes in acute mouse brain slices. Using both chemical and genetically encoded Ca2+ indicators, we demonstrate the complementary advantages of LSFM in mapping Ca2+ domains in astrocyte populations as compared to epifluorescence and two-photon microscopy. Our approach then revealed distinct kinetics of Ca2+ signals between cortical and hypothalamic astrocytes in resting conditions and following the activation of adrenergic G protein coupled receptor (GPCR). This observation highlights the activity heterogeneity across regionally distinct astrocyte populations, and indicates the potential of our method for investigating dynamic signals in astrocytes. |
| first_indexed | 2024-12-23T04:53:48Z |
| format | Article |
| id | doaj.art-33a6e75258fb4915a7b2e05d7df23440 |
| institution | Directory Open Access Journal |
| issn | 1095-9572 |
| language | English |
| last_indexed | 2024-12-23T04:53:48Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | NeuroImage |
| spelling | doaj.art-33a6e75258fb4915a7b2e05d7df234402022-12-21T17:59:24ZengElsevierNeuroImage1095-95722020-10-01220117069Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screeningCuong Pham0Daniela Herrera Moro1Christine Mouffle2Steve Didienne3Régine Hepp4Frank W. Pfrieger5Jean-Marie Mangin6Pascal Legendre7Claire Martin8Serge Luquet9Bruno Cauli10Dongdong Li11Sorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, FranceUnité de Biologie Fonctionnelle et Adaptative, Centre National la Recherche Scientifique, Unité Mixte de Recherche 8251, Université Paris Diderot, Sorbonne Paris Cité, 75205, Paris, FranceSorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, FranceSorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, FranceSorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, FranceCentre National de la Recherche Scientifique, Université de Strasbourg, Institut des Neurosciences Cellulaires et Intégratives, F-67000, Strasbourg, FranceSorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, FranceSorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, FranceUnité de Biologie Fonctionnelle et Adaptative, Centre National la Recherche Scientifique, Unité Mixte de Recherche 8251, Université Paris Diderot, Sorbonne Paris Cité, 75205, Paris, FranceUnité de Biologie Fonctionnelle et Adaptative, Centre National la Recherche Scientifique, Unité Mixte de Recherche 8251, Université Paris Diderot, Sorbonne Paris Cité, 75205, Paris, FranceSorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, FranceSorbonne Université, Institute of Biology Paris Seine, Neuroscience Paris Seine, CNRS UMR8246, INSERM U1130, UPMC UMCR18, Paris, 75005, France; Corresponding author.Astrocytes are a major type of glial cell in the mammalian brain, essentially regulating neuronal development and function. Quantitative imaging represents an important approach to study astrocytic signaling in neural circuits. Focusing on astrocytic Ca2+ activity, a key pathway implicated in astrocye-neuron interaction, we here report a strategy combining fast light sheet fluorescence microscopy (LSFM) and correlative screening-based time series analysis, to map activity domains in astrocytes in living mammalian nerve tissue. Light sheet of micron-scale thickness enables wide-field optical sectioning to image astrocytes in acute mouse brain slices. Using both chemical and genetically encoded Ca2+ indicators, we demonstrate the complementary advantages of LSFM in mapping Ca2+ domains in astrocyte populations as compared to epifluorescence and two-photon microscopy. Our approach then revealed distinct kinetics of Ca2+ signals between cortical and hypothalamic astrocytes in resting conditions and following the activation of adrenergic G protein coupled receptor (GPCR). This observation highlights the activity heterogeneity across regionally distinct astrocyte populations, and indicates the potential of our method for investigating dynamic signals in astrocytes.http://www.sciencedirect.com/science/article/pii/S1053811920305553FluorescenceCalciumGCaMPBrain |
| spellingShingle | Cuong Pham Daniela Herrera Moro Christine Mouffle Steve Didienne Régine Hepp Frank W. Pfrieger Jean-Marie Mangin Pascal Legendre Claire Martin Serge Luquet Bruno Cauli Dongdong Li Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening NeuroImage Fluorescence Calcium GCaMP Brain |
| title | Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening |
| title_full | Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening |
| title_fullStr | Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening |
| title_full_unstemmed | Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening |
| title_short | Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening |
| title_sort | mapping astrocyte activity domains by light sheet imaging and spatio temporal correlation screening |
| topic | Fluorescence Calcium GCaMP Brain |
| url | http://www.sciencedirect.com/science/article/pii/S1053811920305553 |
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