Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia.
Fibropapillomatosis (FP) is a marine turtle disease recognised by benign tumours on the skin, eyes, shell, oral cavity and/or viscera. Despite being a globally distributed disease that affects an endangered species, research on FP and its likely causative agent chelonid alphaherpesvirus 5 (ChHV5) in...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0227268 |
| _version_ | 1818926917642878976 |
|---|---|
| author | K Jones G Burgess A M Budd R Huerlimann N Mashkour E Ariel |
| author_facet | K Jones G Burgess A M Budd R Huerlimann N Mashkour E Ariel |
| author_sort | K Jones |
| collection | DOAJ |
| description | Fibropapillomatosis (FP) is a marine turtle disease recognised by benign tumours on the skin, eyes, shell, oral cavity and/or viscera. Despite being a globally distributed disease that affects an endangered species, research on FP and its likely causative agent chelonid alphaherpesvirus 5 (ChHV5) in Australia is limited. Here we present improved molecular assays developed for detection of ChHV5, in combination with a robust molecular and phylogenetic analysis of ChHV5 variants. This approach utilised a multi-gene assay to detect ChHV5 in all FP tumors sampled from 62 marine turtles found at six foraging grounds along the Great Barrier Reef. Six distinct variants of ChHV5 were identified and the distribution of these variants was associated with host foraging ground. Conversely, no association between host genetic origin and ChHV5 viral variant was found. Together this evidence supports the hypothesis that marine turtles undergo horizontal transmission of ChHV5 at foraging grounds and are unlikely to be contracting the disease at rookeries, either during mating or vertically from parent to offspring. |
| first_indexed | 2024-12-20T03:04:44Z |
| format | Article |
| id | doaj.art-33a979281d614fddb60f850ba04d0dbd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:04:44Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-33a979281d614fddb60f850ba04d0dbd2022-12-21T19:55:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01151e022726810.1371/journal.pone.0227268Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia.K JonesG BurgessA M BuddR HuerlimannN MashkourE ArielFibropapillomatosis (FP) is a marine turtle disease recognised by benign tumours on the skin, eyes, shell, oral cavity and/or viscera. Despite being a globally distributed disease that affects an endangered species, research on FP and its likely causative agent chelonid alphaherpesvirus 5 (ChHV5) in Australia is limited. Here we present improved molecular assays developed for detection of ChHV5, in combination with a robust molecular and phylogenetic analysis of ChHV5 variants. This approach utilised a multi-gene assay to detect ChHV5 in all FP tumors sampled from 62 marine turtles found at six foraging grounds along the Great Barrier Reef. Six distinct variants of ChHV5 were identified and the distribution of these variants was associated with host foraging ground. Conversely, no association between host genetic origin and ChHV5 viral variant was found. Together this evidence supports the hypothesis that marine turtles undergo horizontal transmission of ChHV5 at foraging grounds and are unlikely to be contracting the disease at rookeries, either during mating or vertically from parent to offspring.https://doi.org/10.1371/journal.pone.0227268 |
| spellingShingle | K Jones G Burgess A M Budd R Huerlimann N Mashkour E Ariel Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia. PLoS ONE |
| title | Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia. |
| title_full | Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia. |
| title_fullStr | Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia. |
| title_full_unstemmed | Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia. |
| title_short | Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia. |
| title_sort | molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle chelonia mydas foraging grounds in queensland australia |
| url | https://doi.org/10.1371/journal.pone.0227268 |
| work_keys_str_mv | AT kjones molecularevidenceforhorizontaltransmissionofchelonidalphaherpesvirus5atgreenturtlecheloniamydasforaginggroundsinqueenslandaustralia AT gburgess molecularevidenceforhorizontaltransmissionofchelonidalphaherpesvirus5atgreenturtlecheloniamydasforaginggroundsinqueenslandaustralia AT ambudd molecularevidenceforhorizontaltransmissionofchelonidalphaherpesvirus5atgreenturtlecheloniamydasforaginggroundsinqueenslandaustralia AT rhuerlimann molecularevidenceforhorizontaltransmissionofchelonidalphaherpesvirus5atgreenturtlecheloniamydasforaginggroundsinqueenslandaustralia AT nmashkour molecularevidenceforhorizontaltransmissionofchelonidalphaherpesvirus5atgreenturtlecheloniamydasforaginggroundsinqueenslandaustralia AT eariel molecularevidenceforhorizontaltransmissionofchelonidalphaherpesvirus5atgreenturtlecheloniamydasforaginggroundsinqueenslandaustralia |