Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting
Background:. Autologous fat grafting is increasingly used worldwide and is a very attractive technique in many ways. However, treatment duration and postinjection tissue resorption remain problematic elements, which are largely related to the preparation method used. Moreover, few scientific studies...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2023-10-01
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| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000005349 |
| _version_ | 1797646841345474560 |
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| author | Xavier Nelissen, MD Séverine Licciardi, MS Christophe Nizet, MD Emmanuel Delay, MD Régis Roche, PhD |
| author_facet | Xavier Nelissen, MD Séverine Licciardi, MS Christophe Nizet, MD Emmanuel Delay, MD Régis Roche, PhD |
| author_sort | Xavier Nelissen, MD |
| collection | DOAJ |
| description | Background:. Autologous fat grafting is increasingly used worldwide and is a very attractive technique in many ways. However, treatment duration and postinjection tissue resorption remain problematic elements, which are largely related to the preparation method used. Moreover, few scientific studies objectively compare different fat preparation methods. This study analyzes the efficiency and quality of lipoaspirates prepared with a new filtration/centrifugation system (Adipure) in comparison with several existing techniques.
Methods:. Patient lipoaspirates were processed by five different techniques: decantation, centrifugation, Macrofill, Puregraft, and Adipure. Adipose tissue was evaluated in vitro for tissue resorption and oil formation, as well as in vivo after subcutaneous injections in immunodeficient mice. Adipose grafts were collected after 1 month, weighed, and analyzed by histology with a detailed scoring method.
Results:. Decanting gives inferior results to all other techniques, in terms of amount of tissue and oil in vitro, or graft weight and histological analysis in vivo. Methods using classical Coleman centrifugation (1200g), or a modified one (400g) associated with washes (Macrofill) produce very similar results, both in vitro and in vivo. Techniques using filtration systems (Puregraft and Adipure) produce less oil overall and have a higher grafting efficiency. The best results regarding grafting efficiency and oil quantity are found with the Adipure device.
Conclusions:. A combination of filtration and very low-speed centrifugation potentiates the advantages of these techniques, in terms of graft efficiency. The adipose tissue purification being done in a few minutes, in an automatic way, undoubtedly provides a strong advantage for the use of this new system. |
| first_indexed | 2024-03-11T15:07:39Z |
| format | Article |
| id | doaj.art-33adf54ff4994885b2a53a20b0ad1b52 |
| institution | Directory Open Access Journal |
| issn | 2169-7574 |
| language | English |
| last_indexed | 2024-03-11T15:07:39Z |
| publishDate | 2023-10-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Plastic and Reconstructive Surgery, Global Open |
| spelling | doaj.art-33adf54ff4994885b2a53a20b0ad1b522023-10-30T03:42:32ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742023-10-011110e534910.1097/GOX.0000000000005349202310000-00033Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat GraftingXavier Nelissen, MD0Séverine Licciardi, MS1Christophe Nizet, MD2Emmanuel Delay, MD3Régis Roche, PhD4From the * Department of Plastic, Reconstructive, Maxillofacial Surgery and Burn Center, University Hospital of Liege, Liege, Belgium† Neosyad Company, Aix en Provence, FranceFrom the * Department of Plastic, Reconstructive, Maxillofacial Surgery and Burn Center, University Hospital of Liege, Liege, Belgium‡ Department of Plastic and Reconstructive Surgery, Centre Léon Bérard, Lyon, France.† Neosyad Company, Aix en Provence, FranceBackground:. Autologous fat grafting is increasingly used worldwide and is a very attractive technique in many ways. However, treatment duration and postinjection tissue resorption remain problematic elements, which are largely related to the preparation method used. Moreover, few scientific studies objectively compare different fat preparation methods. This study analyzes the efficiency and quality of lipoaspirates prepared with a new filtration/centrifugation system (Adipure) in comparison with several existing techniques. Methods:. Patient lipoaspirates were processed by five different techniques: decantation, centrifugation, Macrofill, Puregraft, and Adipure. Adipose tissue was evaluated in vitro for tissue resorption and oil formation, as well as in vivo after subcutaneous injections in immunodeficient mice. Adipose grafts were collected after 1 month, weighed, and analyzed by histology with a detailed scoring method. Results:. Decanting gives inferior results to all other techniques, in terms of amount of tissue and oil in vitro, or graft weight and histological analysis in vivo. Methods using classical Coleman centrifugation (1200g), or a modified one (400g) associated with washes (Macrofill) produce very similar results, both in vitro and in vivo. Techniques using filtration systems (Puregraft and Adipure) produce less oil overall and have a higher grafting efficiency. The best results regarding grafting efficiency and oil quantity are found with the Adipure device. Conclusions:. A combination of filtration and very low-speed centrifugation potentiates the advantages of these techniques, in terms of graft efficiency. The adipose tissue purification being done in a few minutes, in an automatic way, undoubtedly provides a strong advantage for the use of this new system.http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000005349 |
| spellingShingle | Xavier Nelissen, MD Séverine Licciardi, MS Christophe Nizet, MD Emmanuel Delay, MD Régis Roche, PhD Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting Plastic and Reconstructive Surgery, Global Open |
| title | Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting |
| title_full | Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting |
| title_fullStr | Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting |
| title_full_unstemmed | Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting |
| title_short | Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting |
| title_sort | comparative analysis of a new automatic system and four existing techniques for autologous fat grafting |
| url | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000005349 |
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