Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review
Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1129195/full |
| _version_ | 1797844981825667072 |
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| author | Kanishka Rangamuwa Kanishka Rangamuwa Christian Aloe Michael Christie Marie-Liesse Asselin-Labat Daniel Batey Lou Irving Thomas John Steven Bozinovski Tracy L. Leong Tracy L. Leong Daniel Steinfort Daniel Steinfort |
| author_facet | Kanishka Rangamuwa Kanishka Rangamuwa Christian Aloe Michael Christie Marie-Liesse Asselin-Labat Daniel Batey Lou Irving Thomas John Steven Bozinovski Tracy L. Leong Tracy L. Leong Daniel Steinfort Daniel Steinfort |
| author_sort | Kanishka Rangamuwa |
| collection | DOAJ |
| description | Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack of response to therapy. Of those that do have an initial response, disease progression may occur due to acquired resistance. The make-up of the tumour microenvironment (TME) and the interaction between tumour infiltrating immune cells and cancer cells can have a large impact on the response to immunotherapy. Robust assessment of the TME with accurate and reproducible methods is vital to understanding mechanisms of immunotherapy resistance. In this paper we will review the evidence of several methodologies to assess the TME, including multiplex immunohistochemistry, imaging mass cytometry, flow cytometry, mass cytometry and RNA sequencing. |
| first_indexed | 2024-04-09T17:31:10Z |
| format | Article |
| id | doaj.art-33adfc9b0eb947d78306dcd6190ad48a |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-09T17:31:10Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-33adfc9b0eb947d78306dcd6190ad48a2023-04-18T05:11:32ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-04-011310.3389/fonc.2023.11291951129195Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative reviewKanishka Rangamuwa0Kanishka Rangamuwa1Christian Aloe2Michael Christie3Marie-Liesse Asselin-Labat4Daniel Batey5Lou Irving6Thomas John7Steven Bozinovski8Tracy L. Leong9Tracy L. Leong10Daniel Steinfort11Daniel Steinfort12Department of Respiratory Medicine, Royal Melbourne Hospital, Melbourne, VIC, AustraliaDepartment of Medicine Royal Melbourne Hospital (RMH), University of Melbourne, Parkville, VIC, AustraliaSchool of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, AustraliaDepartment of Pathology, Royal Melbourne Hospital, Melbourne, VIC, AustraliaPersonalised Oncology Division, Walter Eliza Hall Institute, Melbourne, VIC, AustraliaPersonalised Oncology Division, Walter Eliza Hall Institute, Melbourne, VIC, AustraliaDepartment of Respiratory Medicine, Royal Melbourne Hospital, Melbourne, VIC, AustraliaPeter MacCallum Cancer Centre, Melbourne, VIC, AustraliaSchool of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, AustraliaPersonalised Oncology Division, Walter Eliza Hall Institute, Melbourne, VIC, AustraliaDepartment of Respiratory Medicine, Austin Hospital, Heidelberg, VIC, AustraliaDepartment of Respiratory Medicine, Royal Melbourne Hospital, Melbourne, VIC, AustraliaDepartment of Medicine Royal Melbourne Hospital (RMH), University of Melbourne, Parkville, VIC, AustraliaNon-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Immunotherapy with immune checkpoint inhibitors (ICI) has significantly improved outcomes in some patients, however 80-85% of patients receiving immunotherapy develop primary resistance, manifesting as a lack of response to therapy. Of those that do have an initial response, disease progression may occur due to acquired resistance. The make-up of the tumour microenvironment (TME) and the interaction between tumour infiltrating immune cells and cancer cells can have a large impact on the response to immunotherapy. Robust assessment of the TME with accurate and reproducible methods is vital to understanding mechanisms of immunotherapy resistance. In this paper we will review the evidence of several methodologies to assess the TME, including multiplex immunohistochemistry, imaging mass cytometry, flow cytometry, mass cytometry and RNA sequencing.https://www.frontiersin.org/articles/10.3389/fonc.2023.1129195/fullcytometryimmunohistochemistryRNAseq analysisnon-smal cell lung cancertumour microenvironmentimmune interaction |
| spellingShingle | Kanishka Rangamuwa Kanishka Rangamuwa Christian Aloe Michael Christie Marie-Liesse Asselin-Labat Daniel Batey Lou Irving Thomas John Steven Bozinovski Tracy L. Leong Tracy L. Leong Daniel Steinfort Daniel Steinfort Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review Frontiers in Oncology cytometry immunohistochemistry RNAseq analysis non-smal cell lung cancer tumour microenvironment immune interaction |
| title | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
| title_full | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
| title_fullStr | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
| title_full_unstemmed | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
| title_short | Methods for assessment of the tumour microenvironment and immune interactions in non-small cell lung cancer. A narrative review |
| title_sort | methods for assessment of the tumour microenvironment and immune interactions in non small cell lung cancer a narrative review |
| topic | cytometry immunohistochemistry RNAseq analysis non-smal cell lung cancer tumour microenvironment immune interaction |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1129195/full |
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