Construction of a weight-based seed sorting system for the third-generation hybrid rice
Abstract Background The third-generation hybrid rice technology can be constructed by transforming a recessive nuclear male sterile (NMS) mutant with a transgenic cassette containing three functional modules: the wild type male fertility gene to restore the fertility of the mutant, the pollen killer...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-07-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-021-00510-y |
| _version_ | 1818738962507759616 |
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| author | Jianxin Wu Shijun Qiu Menglong Wang Chunjue Xu Xing Wang Deng Xiaoyan Tang |
| author_facet | Jianxin Wu Shijun Qiu Menglong Wang Chunjue Xu Xing Wang Deng Xiaoyan Tang |
| author_sort | Jianxin Wu |
| collection | DOAJ |
| description | Abstract Background The third-generation hybrid rice technology can be constructed by transforming a recessive nuclear male sterile (NMS) mutant with a transgenic cassette containing three functional modules: the wild type male fertility gene to restore the fertility of the mutant, the pollen killer gene that specifically kills the pollen grains carrying the transgene, and the red fluorescence protein (RFP) gene to mark the transgenic seed (maintainer). The transgenic plant produces 1:1 NMS seeds and maintainer seeds that can be distinguished by the RFP signal. However, the RFP signals in the partially filled or pathogen-infected maintainer seeds are often too weak to be detected by RFP-based seed sorting machine, resulting in intermingling of the maintainer seeds with NMS seeds. Results Here we constructed a weight-based seed sorting system for the third-generation hybrid rice technology by silencing the genes encoding ADP-glucose pyrophosphorylase (AGP) essential for endosperm starch biosynthesis via endosperm-specific expression of artificial microRNAs (amiRNAs). In this system, the NMS seeds have normal endosperm and are heavy, but the maintainer seeds have shrunken endosperms and are light-weighted. The maintainer seeds can be easily and accurately sorted out from the NMS seeds by weight-sorting machines, so pure and fully filled NMS seeds are available. Conclusions The weight-based seed sorting system shows obvious advantages over the RFP-based seed sorting system in accuracy, efficiency, and cost for propagation of pure male sterile seeds. These characteristics will significantly increase the value and transgenic safety of the third-generation hybrid rice technology. |
| first_indexed | 2024-12-18T01:17:16Z |
| format | Article |
| id | doaj.art-33ae1cad7c464b8dbc1ec85a6978630f |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-18T01:17:16Z |
| publishDate | 2021-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-33ae1cad7c464b8dbc1ec85a6978630f2022-12-21T21:25:56ZengSpringerOpenRice1939-84251939-84332021-07-011411710.1186/s12284-021-00510-yConstruction of a weight-based seed sorting system for the third-generation hybrid riceJianxin Wu0Shijun Qiu1Menglong Wang2Chunjue Xu3Xing Wang Deng4Xiaoyan Tang5Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityShenzhen Institute of Molecular Crop DesignShenzhen Institute of Molecular Crop DesignGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityAbstract Background The third-generation hybrid rice technology can be constructed by transforming a recessive nuclear male sterile (NMS) mutant with a transgenic cassette containing three functional modules: the wild type male fertility gene to restore the fertility of the mutant, the pollen killer gene that specifically kills the pollen grains carrying the transgene, and the red fluorescence protein (RFP) gene to mark the transgenic seed (maintainer). The transgenic plant produces 1:1 NMS seeds and maintainer seeds that can be distinguished by the RFP signal. However, the RFP signals in the partially filled or pathogen-infected maintainer seeds are often too weak to be detected by RFP-based seed sorting machine, resulting in intermingling of the maintainer seeds with NMS seeds. Results Here we constructed a weight-based seed sorting system for the third-generation hybrid rice technology by silencing the genes encoding ADP-glucose pyrophosphorylase (AGP) essential for endosperm starch biosynthesis via endosperm-specific expression of artificial microRNAs (amiRNAs). In this system, the NMS seeds have normal endosperm and are heavy, but the maintainer seeds have shrunken endosperms and are light-weighted. The maintainer seeds can be easily and accurately sorted out from the NMS seeds by weight-sorting machines, so pure and fully filled NMS seeds are available. Conclusions The weight-based seed sorting system shows obvious advantages over the RFP-based seed sorting system in accuracy, efficiency, and cost for propagation of pure male sterile seeds. These characteristics will significantly increase the value and transgenic safety of the third-generation hybrid rice technology.https://doi.org/10.1186/s12284-021-00510-yhybrid ricenuclear male sterilityweight-based seed sortingamiRNAOsAGPL2OsAGPS2 |
| spellingShingle | Jianxin Wu Shijun Qiu Menglong Wang Chunjue Xu Xing Wang Deng Xiaoyan Tang Construction of a weight-based seed sorting system for the third-generation hybrid rice Rice hybrid rice nuclear male sterility weight-based seed sorting amiRNA OsAGPL2 OsAGPS2 |
| title | Construction of a weight-based seed sorting system for the third-generation hybrid rice |
| title_full | Construction of a weight-based seed sorting system for the third-generation hybrid rice |
| title_fullStr | Construction of a weight-based seed sorting system for the third-generation hybrid rice |
| title_full_unstemmed | Construction of a weight-based seed sorting system for the third-generation hybrid rice |
| title_short | Construction of a weight-based seed sorting system for the third-generation hybrid rice |
| title_sort | construction of a weight based seed sorting system for the third generation hybrid rice |
| topic | hybrid rice nuclear male sterility weight-based seed sorting amiRNA OsAGPL2 OsAGPS2 |
| url | https://doi.org/10.1186/s12284-021-00510-y |
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