In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer
Objective(s): Conventional methods of cancer treatment include surgery, chemotherapy, radiation therapy, and immunotherapy. Chemotherapy, as one of the main methods of cancer treatment, due to the lack of targeted distribution of the drug in tumor tissues, is not able to destroy cancer cells and als...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-03-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_21648_caf32b99b6d2bb69c2f5416c9e450c30.pdf |
| _version_ | 1797848960766836736 |
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| author | Vahid Abolzadeh Armin Imanparast Hooriyeh Nassirli Naser Tayebi Meybodi Bahareh Khalili Najafabad Ameneh Sazgarnia |
| author_facet | Vahid Abolzadeh Armin Imanparast Hooriyeh Nassirli Naser Tayebi Meybodi Bahareh Khalili Najafabad Ameneh Sazgarnia |
| author_sort | Vahid Abolzadeh |
| collection | DOAJ |
| description | Objective(s): Conventional methods of cancer treatment include surgery, chemotherapy, radiation therapy, and immunotherapy. Chemotherapy, as one of the main methods of cancer treatment, due to the lack of targeted distribution of the drug in tumor tissues, is not able to destroy cancer cells and also affects healthy tissues and causes serious side effects in patients. Sonodynamic therapy (SDT) is a promising strategy for non-invasive treatment of deep solid cancer tumors. In this study, for the first time, the sono-sensitive activity of mitoxantrone was investigated and then mitoxantrone (MTX) was conjugated to hollow gold nanostructure (HGN) to improve the efficiency of in vivo SDT. Materials and Methods: Firstly, after the synthesis of hollow gold nanoshells and the PEGylation process, conjugation of MTX was performed. Then, after evaluating the toxicity of the treatment groups in vitro, in order to perform an in vivo study, 56 male Balb/c mice that had been tumorized by subcutaneous injection of 4T1 cells were divided into eight groups of breast tumor model. Ultrasonic irradiation (US) conditions including intensity of 1.5 W/cm2 (with a frequency of 800 kHz, 5 min), MTX concentration of 2 μM, and HGN dose of 2.5 mg/kg (unit of animal weight) were used.Results: The results show that administration of PEG-HGN-MTX caused a slight reduction in tumor size and growth compared with free MTX. Ultrasound also improved the therapeutic effect of the gold nanoshell in treated groups, and the HGN-PEG-MTX-US treated groups were able to significantly reduce and control tumor size and growth. Conclusion: The findings also show that MTX and HGN can be used as sonosensitizers in SDT. Also, HGN-PEG-MTX can act as a sono-chemotherapy agent for the combination of sonodynamic therapy and chemotherapy for in vivo breast tumors. |
| first_indexed | 2024-04-09T18:36:04Z |
| format | Article |
| id | doaj.art-33b42502b77d4a5e82329844470cb0cb |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-09T18:36:04Z |
| publishDate | 2023-03-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-33b42502b77d4a5e82329844470cb0cb2023-04-11T09:30:51ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-03-0126328529410.22038/ijbms.2023.67602.1481121648In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancerVahid Abolzadeh0Armin Imanparast1Hooriyeh Nassirli2Naser Tayebi Meybodi3Bahareh Khalili Najafabad4Ameneh Sazgarnia5Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranMedical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMedical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranMedical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Conventional methods of cancer treatment include surgery, chemotherapy, radiation therapy, and immunotherapy. Chemotherapy, as one of the main methods of cancer treatment, due to the lack of targeted distribution of the drug in tumor tissues, is not able to destroy cancer cells and also affects healthy tissues and causes serious side effects in patients. Sonodynamic therapy (SDT) is a promising strategy for non-invasive treatment of deep solid cancer tumors. In this study, for the first time, the sono-sensitive activity of mitoxantrone was investigated and then mitoxantrone (MTX) was conjugated to hollow gold nanostructure (HGN) to improve the efficiency of in vivo SDT. Materials and Methods: Firstly, after the synthesis of hollow gold nanoshells and the PEGylation process, conjugation of MTX was performed. Then, after evaluating the toxicity of the treatment groups in vitro, in order to perform an in vivo study, 56 male Balb/c mice that had been tumorized by subcutaneous injection of 4T1 cells were divided into eight groups of breast tumor model. Ultrasonic irradiation (US) conditions including intensity of 1.5 W/cm2 (with a frequency of 800 kHz, 5 min), MTX concentration of 2 μM, and HGN dose of 2.5 mg/kg (unit of animal weight) were used.Results: The results show that administration of PEG-HGN-MTX caused a slight reduction in tumor size and growth compared with free MTX. Ultrasound also improved the therapeutic effect of the gold nanoshell in treated groups, and the HGN-PEG-MTX-US treated groups were able to significantly reduce and control tumor size and growth. Conclusion: The findings also show that MTX and HGN can be used as sonosensitizers in SDT. Also, HGN-PEG-MTX can act as a sono-chemotherapy agent for the combination of sonodynamic therapy and chemotherapy for in vivo breast tumors.https://ijbms.mums.ac.ir/article_21648_caf32b99b6d2bb69c2f5416c9e450c30.pdfbreast cancerchemotherapycombination therapyhollow gold nanoshellmitoxantronesonodynamic therapy |
| spellingShingle | Vahid Abolzadeh Armin Imanparast Hooriyeh Nassirli Naser Tayebi Meybodi Bahareh Khalili Najafabad Ameneh Sazgarnia In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer Iranian Journal of Basic Medical Sciences breast cancer chemotherapy combination therapy hollow gold nanoshell mitoxantrone sonodynamic therapy |
| title | In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer |
| title_full | In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer |
| title_fullStr | In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer |
| title_full_unstemmed | In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer |
| title_short | In vivo evaluation of Sono-chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer |
| title_sort | in vivo evaluation of sono chemo therapy via hollow gold nanoshells conjugated to mitoxantrone on breast cancer |
| topic | breast cancer chemotherapy combination therapy hollow gold nanoshell mitoxantrone sonodynamic therapy |
| url | https://ijbms.mums.ac.ir/article_21648_caf32b99b6d2bb69c2f5416c9e450c30.pdf |
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