Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication
The aim of this study was to investigate the effect of ultrasonic stress germination (USG) on total phenolic contents (TPC), total flavonoid contents (TFC), the phenolic compositions, and antioxidant activities of black highland barley (BHB). The USG processing parameters, polyphenol profile, phenol...
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MDPI AG
2023-04-01
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Online Access: | https://www.mdpi.com/1420-3049/28/9/3679 |
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author | Jie Zhang Junling Guo Bin Dang Wengang Zhang Wancai Zheng Xijuan Yang |
author_facet | Jie Zhang Junling Guo Bin Dang Wengang Zhang Wancai Zheng Xijuan Yang |
author_sort | Jie Zhang |
collection | DOAJ |
description | The aim of this study was to investigate the effect of ultrasonic stress germination (USG) on total phenolic contents (TPC), total flavonoid contents (TFC), the phenolic compositions, and antioxidant activities of black highland barley (BHB). The USG processing parameters, polyphenol profile, phenolic compositions, and antioxidant activities were explored after USG. Results showed that the optimal USG parameters were as follows: 350 W ultrasonic pretreatment power, 30 °C ultrasonication temperature, 25 min ultrasonication time, and 64 h germination time. Under these conditions, the total phenolic content (688.84 ± 5.30 mg/100 g) and total flavonoid content (59.23 ± 0.45 mg/100 g) of BHB were increased by 28.55% and 10.15%, respectively, compared to the untreated samples. In addition, the USG treatment could more effectively enrich bound phenolic acids and free flavonoids, among which the content of catechin was significantly increased by USG and was the main characteristic substance. Moreover, the USG treatment could improve the antioxidant activity and had a higher antioxidant potency composite index (APC index) (97.91%) of BHB. These results indicate that USG might be an effective method to enrich polyphenols and improve antioxidant activity in BHB. |
first_indexed | 2024-03-11T04:11:58Z |
format | Article |
id | doaj.art-33b46d20d5a7409c8714302e34a226a9 |
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issn | 1420-3049 |
language | English |
last_indexed | 2024-03-11T04:11:58Z |
publishDate | 2023-04-01 |
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spelling | doaj.art-33b46d20d5a7409c8714302e34a226a92023-11-17T23:22:02ZengMDPI AGMolecules1420-30492023-04-01289367910.3390/molecules28093679Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by UltrasonicationJie Zhang0Junling Guo1Bin Dang2Wengang Zhang3Wancai Zheng4Xijuan Yang5Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaQinghai Tibetan Plateau Key Laboratory of Agricultural Product Processing, Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaThe aim of this study was to investigate the effect of ultrasonic stress germination (USG) on total phenolic contents (TPC), total flavonoid contents (TFC), the phenolic compositions, and antioxidant activities of black highland barley (BHB). The USG processing parameters, polyphenol profile, phenolic compositions, and antioxidant activities were explored after USG. Results showed that the optimal USG parameters were as follows: 350 W ultrasonic pretreatment power, 30 °C ultrasonication temperature, 25 min ultrasonication time, and 64 h germination time. Under these conditions, the total phenolic content (688.84 ± 5.30 mg/100 g) and total flavonoid content (59.23 ± 0.45 mg/100 g) of BHB were increased by 28.55% and 10.15%, respectively, compared to the untreated samples. In addition, the USG treatment could more effectively enrich bound phenolic acids and free flavonoids, among which the content of catechin was significantly increased by USG and was the main characteristic substance. Moreover, the USG treatment could improve the antioxidant activity and had a higher antioxidant potency composite index (APC index) (97.91%) of BHB. These results indicate that USG might be an effective method to enrich polyphenols and improve antioxidant activity in BHB.https://www.mdpi.com/1420-3049/28/9/3679black highland barleypolyphenolsultrasoundgerminationantioxidant activity |
spellingShingle | Jie Zhang Junling Guo Bin Dang Wengang Zhang Wancai Zheng Xijuan Yang Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication Molecules black highland barley polyphenols ultrasound germination antioxidant activity |
title | Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication |
title_full | Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication |
title_fullStr | Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication |
title_full_unstemmed | Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication |
title_short | Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication |
title_sort | enhancement of polyphenols and antioxidant activity in germinated black highland barley by ultrasonication |
topic | black highland barley polyphenols ultrasound germination antioxidant activity |
url | https://www.mdpi.com/1420-3049/28/9/3679 |
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