Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication

The aim of this study was to investigate the effect of ultrasonic stress germination (USG) on total phenolic contents (TPC), total flavonoid contents (TFC), the phenolic compositions, and antioxidant activities of black highland barley (BHB). The USG processing parameters, polyphenol profile, phenol...

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Main Authors: Jie Zhang, Junling Guo, Bin Dang, Wengang Zhang, Wancai Zheng, Xijuan Yang
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/28/9/3679
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author Jie Zhang
Junling Guo
Bin Dang
Wengang Zhang
Wancai Zheng
Xijuan Yang
author_facet Jie Zhang
Junling Guo
Bin Dang
Wengang Zhang
Wancai Zheng
Xijuan Yang
author_sort Jie Zhang
collection DOAJ
description The aim of this study was to investigate the effect of ultrasonic stress germination (USG) on total phenolic contents (TPC), total flavonoid contents (TFC), the phenolic compositions, and antioxidant activities of black highland barley (BHB). The USG processing parameters, polyphenol profile, phenolic compositions, and antioxidant activities were explored after USG. Results showed that the optimal USG parameters were as follows: 350 W ultrasonic pretreatment power, 30 °C ultrasonication temperature, 25 min ultrasonication time, and 64 h germination time. Under these conditions, the total phenolic content (688.84 ± 5.30 mg/100 g) and total flavonoid content (59.23 ± 0.45 mg/100 g) of BHB were increased by 28.55% and 10.15%, respectively, compared to the untreated samples. In addition, the USG treatment could more effectively enrich bound phenolic acids and free flavonoids, among which the content of catechin was significantly increased by USG and was the main characteristic substance. Moreover, the USG treatment could improve the antioxidant activity and had a higher antioxidant potency composite index (APC index) (97.91%) of BHB. These results indicate that USG might be an effective method to enrich polyphenols and improve antioxidant activity in BHB.
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spelling doaj.art-33b46d20d5a7409c8714302e34a226a92023-11-17T23:22:02ZengMDPI AGMolecules1420-30492023-04-01289367910.3390/molecules28093679Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by UltrasonicationJie Zhang0Junling Guo1Bin Dang2Wengang Zhang3Wancai Zheng4Xijuan Yang5Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaQinghai Tibetan Plateau Key Laboratory of Agricultural Product Processing, Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, ChinaThe aim of this study was to investigate the effect of ultrasonic stress germination (USG) on total phenolic contents (TPC), total flavonoid contents (TFC), the phenolic compositions, and antioxidant activities of black highland barley (BHB). The USG processing parameters, polyphenol profile, phenolic compositions, and antioxidant activities were explored after USG. Results showed that the optimal USG parameters were as follows: 350 W ultrasonic pretreatment power, 30 °C ultrasonication temperature, 25 min ultrasonication time, and 64 h germination time. Under these conditions, the total phenolic content (688.84 ± 5.30 mg/100 g) and total flavonoid content (59.23 ± 0.45 mg/100 g) of BHB were increased by 28.55% and 10.15%, respectively, compared to the untreated samples. In addition, the USG treatment could more effectively enrich bound phenolic acids and free flavonoids, among which the content of catechin was significantly increased by USG and was the main characteristic substance. Moreover, the USG treatment could improve the antioxidant activity and had a higher antioxidant potency composite index (APC index) (97.91%) of BHB. These results indicate that USG might be an effective method to enrich polyphenols and improve antioxidant activity in BHB.https://www.mdpi.com/1420-3049/28/9/3679black highland barleypolyphenolsultrasoundgerminationantioxidant activity
spellingShingle Jie Zhang
Junling Guo
Bin Dang
Wengang Zhang
Wancai Zheng
Xijuan Yang
Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication
Molecules
black highland barley
polyphenols
ultrasound
germination
antioxidant activity
title Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication
title_full Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication
title_fullStr Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication
title_full_unstemmed Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication
title_short Enhancement of Polyphenols and Antioxidant Activity in Germinated Black Highland Barley by Ultrasonication
title_sort enhancement of polyphenols and antioxidant activity in germinated black highland barley by ultrasonication
topic black highland barley
polyphenols
ultrasound
germination
antioxidant activity
url https://www.mdpi.com/1420-3049/28/9/3679
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