L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation
ABSTRACTObjective: Methotrexate (MTX) is a drug used in the treatment of cancer and autoimmune disorders; however, its clinical use is limited because of serious side effects including renal toxicity. This study aimed to investigate the protective effect of Lmethionine (L-Met) on MTX toxicity in the...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2023.2270886 |
| _version_ | 1797398757233393664 |
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| author | Wessam M. Abdel-Wahab Nada S. Daifalla Amina E. Essawy |
| author_facet | Wessam M. Abdel-Wahab Nada S. Daifalla Amina E. Essawy |
| author_sort | Wessam M. Abdel-Wahab |
| collection | DOAJ |
| description | ABSTRACTObjective: Methotrexate (MTX) is a drug used in the treatment of cancer and autoimmune disorders; however, its clinical use is limited because of serious side effects including renal toxicity. This study aimed to investigate the protective effect of Lmethionine (L-Met) on MTX toxicity in the kidneys of rats.Methods: Thirty male rats were divided equally into five groups: control (saline), Met400 (400 mg/kg L-Met), MTX (20 mg/kg MTX), MTX-Met300 (300 mg/kg L-Met and 20 mg/kg MTX), and MTX-Met400 (400 mg/kg L-Met and 20 mg/kg MTX). Rats were euthanized one day after the last dose administration (day 16) and serum and renal tissue samples were collected. Renal function and injury indices, oxidative stress/antioxidant indices and proinflammatory cytokines were evaluated.Results: The results showed that L-Met could effectively counteract the nephrotoxic effects of MTX, in a dose-related manner, by improving most of the tested parameters. Furthermore, the higher dose of L-Met was able to restore several parameters to normal levels. In addition, investigation of MTX-induced hematological changes revealed a corrective potential of L-Met.Conclusion: L-Met can be an effective adjuvant therapy to modulate renal toxicity associated with MTX because of its antioxidant and antiinflammatory effects. |
| first_indexed | 2024-03-09T01:30:07Z |
| format | Article |
| id | doaj.art-33b4bdb1fa6d49dc989395c5efbf6cae |
| institution | Directory Open Access Journal |
| issn | 1351-0002 1743-2928 |
| language | English |
| last_indexed | 2024-03-09T01:30:07Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj.art-33b4bdb1fa6d49dc989395c5efbf6cae2023-12-09T20:05:02ZengTaylor & Francis GroupRedox Report1351-00021743-29282023-12-0128110.1080/13510002.2023.2270886L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammationWessam M. Abdel-Wahab0Nada S. Daifalla1Amina E. Essawy2Department of Zoology, Faculty of Science, Alexandria University, Alexandria, EgyptDepartment of Basic Sciences, Deanship of Preparatory Year and Supporting Studies, Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaDepartment of Zoology, Faculty of Science, Alexandria University, Alexandria, EgyptABSTRACTObjective: Methotrexate (MTX) is a drug used in the treatment of cancer and autoimmune disorders; however, its clinical use is limited because of serious side effects including renal toxicity. This study aimed to investigate the protective effect of Lmethionine (L-Met) on MTX toxicity in the kidneys of rats.Methods: Thirty male rats were divided equally into five groups: control (saline), Met400 (400 mg/kg L-Met), MTX (20 mg/kg MTX), MTX-Met300 (300 mg/kg L-Met and 20 mg/kg MTX), and MTX-Met400 (400 mg/kg L-Met and 20 mg/kg MTX). Rats were euthanized one day after the last dose administration (day 16) and serum and renal tissue samples were collected. Renal function and injury indices, oxidative stress/antioxidant indices and proinflammatory cytokines were evaluated.Results: The results showed that L-Met could effectively counteract the nephrotoxic effects of MTX, in a dose-related manner, by improving most of the tested parameters. Furthermore, the higher dose of L-Met was able to restore several parameters to normal levels. In addition, investigation of MTX-induced hematological changes revealed a corrective potential of L-Met.Conclusion: L-Met can be an effective adjuvant therapy to modulate renal toxicity associated with MTX because of its antioxidant and antiinflammatory effects.https://www.tandfonline.com/doi/10.1080/13510002.2023.2270886Methotrexaterenal toxicityredox statusinflammationhematological parametersL-methionine |
| spellingShingle | Wessam M. Abdel-Wahab Nada S. Daifalla Amina E. Essawy L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation Redox Report Methotrexate renal toxicity redox status inflammation hematological parameters L-methionine |
| title | L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation |
| title_full | L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation |
| title_fullStr | L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation |
| title_full_unstemmed | L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation |
| title_short | L-methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation |
| title_sort | l methionine protects against nephrotoxicity induced by methotrexate through modulation of redox status and inflammation |
| topic | Methotrexate renal toxicity redox status inflammation hematological parameters L-methionine |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2023.2270886 |
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