A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.)
<p>Abstract</p> <p>Background</p> <p>The sweet, floral flavor typical of Muscat varieties (Muscats), due to high levels of monoterpenoids (geraniol, linalool and nerol), is highly distinct and has been greatly appreciated both in table grapes and in wine since ancient t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-11-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/241 |
| _version_ | 1819036089386532864 |
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| author | Boursiquot Jean-Michel Le Cunff Loïc Costantini Laura Battilana Juri Emanuelli Francesco This Patrice Grando Maria S |
| author_facet | Boursiquot Jean-Michel Le Cunff Loïc Costantini Laura Battilana Juri Emanuelli Francesco This Patrice Grando Maria S |
| author_sort | Boursiquot Jean-Michel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The sweet, floral flavor typical of Muscat varieties (Muscats), due to high levels of monoterpenoids (geraniol, linalool and nerol), is highly distinct and has been greatly appreciated both in table grapes and in wine since ancient times. Muscat flavor determination in grape (<it>Vitis vinifera </it>L.) has up to now been studied by evaluating monoterpenoid levels through QTL analysis. These studies have revealed co-localization of 1-deoxy-D-xylulose 5-phosphate synthase (<it>VvDXS</it>) with the major QTL positioned on chromosome 5.</p> <p>Results</p> <p>We resequenced <it>VvDXS </it>in an <it>ad hoc </it>association population of 148 grape varieties, which included muscat-flavored, aromatic and neutral accessions as well as muscat-like aromatic mutants and non-aromatic offsprings of Muscats. Gene nucleotide diversity and intragenic linkage disequilibrium (LD) were evaluated. Structured association analysis revealed three SNPs in moderate LD to be significantly associated with muscat-flavored varieties. We identified a putative causal SNP responsible for a predicted non-neutral substitution and we discuss its possible implications for flavor metabolism. Network analysis revealed a major star-shaped cluster of reconstructed haplotypes unique to muscat-flavored varieties. Moreover, muscat-like aromatic mutants displayed unique non-synonymous mutations near the mutated site of Muscat genotypes.</p> <p>Conclusions</p> <p>This study is a crucial step forward in understanding the genetic regulation of muscat flavor in grapevine and it also sheds light on the domestication history of Muscats. <it>VvDXS </it>appears to be a possible human-selected locus in grapevine domestication and post-domestication. The putative causal SNP identified in Muscat varieties as well as the unique mutations identifying the muscat-like aromatic mutants under study may be immediately applied in marker-assisted breeding programs aimed at enhancing fragrance and aroma complexity respectively in table grape and wine cultivars.</p> |
| first_indexed | 2024-12-21T07:59:59Z |
| format | Article |
| id | doaj.art-33ba8c43ddc64cfd93805e9b5ab71bbc |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T07:59:59Z |
| publishDate | 2010-11-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-33ba8c43ddc64cfd93805e9b5ab71bbc2022-12-21T19:10:54ZengBMCBMC Plant Biology1471-22292010-11-0110124110.1186/1471-2229-10-241A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.)Boursiquot Jean-MichelLe Cunff LoïcCostantini LauraBattilana JuriEmanuelli FrancescoThis PatriceGrando Maria S<p>Abstract</p> <p>Background</p> <p>The sweet, floral flavor typical of Muscat varieties (Muscats), due to high levels of monoterpenoids (geraniol, linalool and nerol), is highly distinct and has been greatly appreciated both in table grapes and in wine since ancient times. Muscat flavor determination in grape (<it>Vitis vinifera </it>L.) has up to now been studied by evaluating monoterpenoid levels through QTL analysis. These studies have revealed co-localization of 1-deoxy-D-xylulose 5-phosphate synthase (<it>VvDXS</it>) with the major QTL positioned on chromosome 5.</p> <p>Results</p> <p>We resequenced <it>VvDXS </it>in an <it>ad hoc </it>association population of 148 grape varieties, which included muscat-flavored, aromatic and neutral accessions as well as muscat-like aromatic mutants and non-aromatic offsprings of Muscats. Gene nucleotide diversity and intragenic linkage disequilibrium (LD) were evaluated. Structured association analysis revealed three SNPs in moderate LD to be significantly associated with muscat-flavored varieties. We identified a putative causal SNP responsible for a predicted non-neutral substitution and we discuss its possible implications for flavor metabolism. Network analysis revealed a major star-shaped cluster of reconstructed haplotypes unique to muscat-flavored varieties. Moreover, muscat-like aromatic mutants displayed unique non-synonymous mutations near the mutated site of Muscat genotypes.</p> <p>Conclusions</p> <p>This study is a crucial step forward in understanding the genetic regulation of muscat flavor in grapevine and it also sheds light on the domestication history of Muscats. <it>VvDXS </it>appears to be a possible human-selected locus in grapevine domestication and post-domestication. The putative causal SNP identified in Muscat varieties as well as the unique mutations identifying the muscat-like aromatic mutants under study may be immediately applied in marker-assisted breeding programs aimed at enhancing fragrance and aroma complexity respectively in table grape and wine cultivars.</p>http://www.biomedcentral.com/1471-2229/10/241 |
| spellingShingle | Boursiquot Jean-Michel Le Cunff Loïc Costantini Laura Battilana Juri Emanuelli Francesco This Patrice Grando Maria S A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.) BMC Plant Biology |
| title | A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.) |
| title_full | A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.) |
| title_fullStr | A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.) |
| title_full_unstemmed | A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.) |
| title_short | A candidate gene association study on muscat flavor in grapevine (<it>Vitis vinifera </it>L.) |
| title_sort | candidate gene association study on muscat flavor in grapevine it vitis vinifera it l |
| url | http://www.biomedcentral.com/1471-2229/10/241 |
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