CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients
Abstract Background Early detection of capecitabine-resistance could largely increase overall survival of colorectal cancer (CRC) patients. Previous studies suggested examination of immune cells in peripheral blood would help to predict efficacy of chemotherapy. Methods We examined the immunological...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | BMC Immunology |
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| Online Access: | http://link.springer.com/article/10.1186/s12865-020-00375-8 |
| _version_ | 1818190940003631104 |
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| author | Yu Lu Yizhou Huang Lei Huang Yanjie Xu Zien Wang Han Li Ting Zhang Ming Zhong Wei-qiang Gao Yan Zhang |
| author_facet | Yu Lu Yizhou Huang Lei Huang Yanjie Xu Zien Wang Han Li Ting Zhang Ming Zhong Wei-qiang Gao Yan Zhang |
| author_sort | Yu Lu |
| collection | DOAJ |
| description | Abstract Background Early detection of capecitabine-resistance could largely increase overall survival of colorectal cancer (CRC) patients. Previous studies suggested examination of immune cells in peripheral blood would help to predict efficacy of chemotherapy. Methods We examined the immunological characteristics of peripheral blood in CRC patients with capecitabine treatment. We analyzed the relationships between the abnormal immune cell population in capecitabine-resistance patients and major clinical features. Furthermore, RNA sequencing, analyses of cell surface marker expression and the correlations with other major immune cell populations were performed using this population to explore the possible function of these cells. Results The expression level of CD16 on neutrophils was down-regulated in capecitabine-resistant CRC patients. Patients with CD16low/−neutrophils after capecitabine therapy had adverse clinical features. What’s important, the change of CD16 expression level on neutrophils appeared much earlier than CT scan. RNA sequencing revealed that CD16low/−neutrophils in capecitabine-resistant patients had lower expression level of neutrophil-related genes, compared to CD16+neutrophils in capecitabine-sensitive patients, suggesting this CD16low/−population might be immature neutrophils. Furthermore, the expression level of CD16 on neutrophils in patients with capecitabine treatment was positively correlated with the number of anti-tumor immune cell subsets, such as CD8+T cell, CD4+T cell, NK cell and monocyte. Conclusions Our findings indicated that CD16 expression on neutrophils in peripheral blood was a good prognostic marker for predicting efficacy of capecitabine in CRC patients. |
| first_indexed | 2024-12-12T00:06:41Z |
| format | Article |
| id | doaj.art-33baa728de5e404caa44b4c172266797 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-12T00:06:41Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-33baa728de5e404caa44b4c1722667972022-12-22T00:45:06ZengBMCBMC Immunology1471-21722020-08-0121111410.1186/s12865-020-00375-8CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patientsYu Lu0Yizhou Huang1Lei Huang2Yanjie Xu3Zien Wang4Han Li5Ting Zhang6Ming Zhong7Wei-qiang Gao8Yan Zhang9State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiaotong UniversityDepartment of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong UniversityMed-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong UniversityMed-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong UniversityMed-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong UniversityMed-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong UniversityMed-X Research Institute & School of Biomedical Engineering, Shanghai Jiaotong UniversityDepartment of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-Med X Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiaotong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-Med X Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiaotong UniversityAbstract Background Early detection of capecitabine-resistance could largely increase overall survival of colorectal cancer (CRC) patients. Previous studies suggested examination of immune cells in peripheral blood would help to predict efficacy of chemotherapy. Methods We examined the immunological characteristics of peripheral blood in CRC patients with capecitabine treatment. We analyzed the relationships between the abnormal immune cell population in capecitabine-resistance patients and major clinical features. Furthermore, RNA sequencing, analyses of cell surface marker expression and the correlations with other major immune cell populations were performed using this population to explore the possible function of these cells. Results The expression level of CD16 on neutrophils was down-regulated in capecitabine-resistant CRC patients. Patients with CD16low/−neutrophils after capecitabine therapy had adverse clinical features. What’s important, the change of CD16 expression level on neutrophils appeared much earlier than CT scan. RNA sequencing revealed that CD16low/−neutrophils in capecitabine-resistant patients had lower expression level of neutrophil-related genes, compared to CD16+neutrophils in capecitabine-sensitive patients, suggesting this CD16low/−population might be immature neutrophils. Furthermore, the expression level of CD16 on neutrophils in patients with capecitabine treatment was positively correlated with the number of anti-tumor immune cell subsets, such as CD8+T cell, CD4+T cell, NK cell and monocyte. Conclusions Our findings indicated that CD16 expression on neutrophils in peripheral blood was a good prognostic marker for predicting efficacy of capecitabine in CRC patients.http://link.springer.com/article/10.1186/s12865-020-00375-8CD16NeutrophilsCapecitabine-resistanceColorectal cancer |
| spellingShingle | Yu Lu Yizhou Huang Lei Huang Yanjie Xu Zien Wang Han Li Ting Zhang Ming Zhong Wei-qiang Gao Yan Zhang CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients BMC Immunology CD16 Neutrophils Capecitabine-resistance Colorectal cancer |
| title | CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients |
| title_full | CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients |
| title_fullStr | CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients |
| title_full_unstemmed | CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients |
| title_short | CD16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients |
| title_sort | cd16 expression on neutrophils predicts treatment efficacy of capecitabine in colorectal cancer patients |
| topic | CD16 Neutrophils Capecitabine-resistance Colorectal cancer |
| url | http://link.springer.com/article/10.1186/s12865-020-00375-8 |
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