Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep
Blood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/5/229 |
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| author | Rejane Santos Sousa Caroline Santos Sousa Francisco Leonardo Costa Oliveira Paulo Ricardo Firmino Isadora Karolina Freitas Sousa Valeria Veras Paula Nohora Mercado Caruso Enrico Lippi Ortolani Antonio Humberto Hamad Minervino Raimundo Alves Barrêto-Júnior |
| author_facet | Rejane Santos Sousa Caroline Santos Sousa Francisco Leonardo Costa Oliveira Paulo Ricardo Firmino Isadora Karolina Freitas Sousa Valeria Veras Paula Nohora Mercado Caruso Enrico Lippi Ortolani Antonio Humberto Hamad Minervino Raimundo Alves Barrêto-Júnior |
| author_sort | Rejane Santos Sousa |
| collection | DOAJ |
| description | Blood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluated clinically and by laboratory tests for clinical, biochemical, and blood gas variables and to assess oxidative stress before induction (T0), 30 min (T30 min), and 6 (T6 h), 12 (T12 h), and 24 h (T24 h) after blood loss. The sheep showed tachycardia from T30 min until T24 h, reduction in the hematocrit, number of erythrocytes, and hemoglobin concentration, with lower values at T24 h and increase in the number of leukocytes from T12 h on. There was a reduction in blood pH and oxygen pressure at T30 min, increased lactate concentration and reduced blood bicarbonate at this time. There was an increase in urea concentration from T6 h until the end of the study, with no change in creatinine levels. The animals did not show changes in the concentration of malonaldehyde, and in the activity of the enzymes superoxide dismutase, glutathione peroxidase, and catalase, but there was a reduction in the concentration of reduced glutathione at T24 h. The acute loss of 40% of blood volume is capable of promoting relevant clinical, hematological, blood gas, and biochemical alterations, and contributed to the appearance of oxidative stress with reduced glutathione concentration, suggesting that this process generated free radicals in sufficient quantity to diminish the action of antioxidants. |
| first_indexed | 2024-03-10T01:38:12Z |
| format | Article |
| id | doaj.art-33bb836f6f0a4283a6da7d5f4c9551d3 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T01:38:12Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-33bb836f6f0a4283a6da7d5f4c9551d32023-11-23T13:28:37ZengMDPI AGVeterinary Sciences2306-73812022-05-019522910.3390/vetsci9050229Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in SheepRejane Santos Sousa0Caroline Santos Sousa1Francisco Leonardo Costa Oliveira2Paulo Ricardo Firmino3Isadora Karolina Freitas Sousa4Valeria Veras Paula5Nohora Mercado Caruso6Enrico Lippi Ortolani7Antonio Humberto Hamad Minervino8Raimundo Alves Barrêto-Júnior9School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo 05508-270, SP, BrazilSchool of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo 05508-270, SP, BrazilSchool of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo 05508-270, SP, BrazilDepartment of Animal Science, Federal Rural University of the Semiarid Region, Av. Francisco Mota 572, Bairro Costa e Silva, Mossoró 59625-000, RN, BrazilSchool of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo 05508-270, SP, BrazilDepartment of Animal Science, Federal Rural University of the Semiarid Region, Av. Francisco Mota 572, Bairro Costa e Silva, Mossoró 59625-000, RN, BrazilDepartamento Productividad e Innovación, Universidad de la Costa (CUC), Calle 58 n.55-66, Barranquilla 080001, ColombiaSchool of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo 05508-270, SP, BrazilLaboratory of Animal Health, LARSANA, Federal University of Western Pará (UFOPA), Rua Vera Paz S/N, Salé, Santarém 68040-255, PA, BrazilDepartment of Animal Science, Federal Rural University of the Semiarid Region, Av. Francisco Mota 572, Bairro Costa e Silva, Mossoró 59625-000, RN, BrazilBlood loss in sheep can have different causes and may result in anemia. We aimed to evaluate the clinical, hematological, and biochemical alterations and the oxidative stress generated by acute blood loss. Eighteen healthy sheep underwent phlebotomy to remove 40% of the blood volume and were evaluated clinically and by laboratory tests for clinical, biochemical, and blood gas variables and to assess oxidative stress before induction (T0), 30 min (T30 min), and 6 (T6 h), 12 (T12 h), and 24 h (T24 h) after blood loss. The sheep showed tachycardia from T30 min until T24 h, reduction in the hematocrit, number of erythrocytes, and hemoglobin concentration, with lower values at T24 h and increase in the number of leukocytes from T12 h on. There was a reduction in blood pH and oxygen pressure at T30 min, increased lactate concentration and reduced blood bicarbonate at this time. There was an increase in urea concentration from T6 h until the end of the study, with no change in creatinine levels. The animals did not show changes in the concentration of malonaldehyde, and in the activity of the enzymes superoxide dismutase, glutathione peroxidase, and catalase, but there was a reduction in the concentration of reduced glutathione at T24 h. The acute loss of 40% of blood volume is capable of promoting relevant clinical, hematological, blood gas, and biochemical alterations, and contributed to the appearance of oxidative stress with reduced glutathione concentration, suggesting that this process generated free radicals in sufficient quantity to diminish the action of antioxidants.https://www.mdpi.com/2306-7381/9/5/229anemiaovinesuperoxide dismutaseglutathione peroxidasecatalase |
| spellingShingle | Rejane Santos Sousa Caroline Santos Sousa Francisco Leonardo Costa Oliveira Paulo Ricardo Firmino Isadora Karolina Freitas Sousa Valeria Veras Paula Nohora Mercado Caruso Enrico Lippi Ortolani Antonio Humberto Hamad Minervino Raimundo Alves Barrêto-Júnior Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep Veterinary Sciences anemia ovine superoxide dismutase glutathione peroxidase catalase |
| title | Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep |
| title_full | Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep |
| title_fullStr | Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep |
| title_full_unstemmed | Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep |
| title_short | Impact of Acute Blood Loss on Clinical, Hematological, Biochemical, and Oxidative Stress Variables in Sheep |
| title_sort | impact of acute blood loss on clinical hematological biochemical and oxidative stress variables in sheep |
| topic | anemia ovine superoxide dismutase glutathione peroxidase catalase |
| url | https://www.mdpi.com/2306-7381/9/5/229 |
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