CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals
The 32-bp deletion in the HIV-1 co-receptor CCR5 confers a high degree of resistance to HIV-1 infection in homozygous individuals for the deleted allele and partial protection against HIV-1 during disease progression in heterozygotes. Natural ligands for CCR5, MIP-1alpha, MIP-1ß and RANTES, have bee...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2002-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100011 |
| _version_ | 1818275242184802304 |
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| author | Mikawa A.Y. Tagliavini S.A. Costa P.I. |
| author_facet | Mikawa A.Y. Tagliavini S.A. Costa P.I. |
| author_sort | Mikawa A.Y. |
| collection | DOAJ |
| description | The 32-bp deletion in the HIV-1 co-receptor CCR5 confers a high degree of resistance to HIV-1 infection in homozygous individuals for the deleted allele and partial protection against HIV-1 during disease progression in heterozygotes. Natural ligands for CCR5, MIP-1alpha, MIP-1ß and RANTES, have been shown to inhibit HIV replication in CD4+ T cells. In the present study, we examined the CCR5 genotype by PCR and the plasma levels of RANTES and MIP-1alpha by ELISA among blood donors (N = 26) and among HIV-1-infected individuals (N = 129). The control group consisted of healthy adult volunteers and HIV-1-infected subjects were an asymptomatic and heterogeneous group of individuals with regard to immunologic and virologic markers of HIV-1 disease. The frequency of the CCR5 mutant allele (delta32ccr5) in this population was 0.032; however, no delta32ccr5 homozygote was detected. These results could be related to the intense ethnic admixture of the Brazilian population. There was no correlation between circulating ß-chemokines (MIP-1alpha, RANTES) and viral load in HIV-infected individuals. RANTES concentrations in plasma samples from HIV+ patients carrying the homozygous CCR5 allele (CCR5/CCR5) (28.23 ng/ml) were higher than in the control samples (16.07 ng/ml; P<0.05); however, this HIV+ patient group (mean 26.23 pg/ml) had significantly lower concentrations of MIP-1alpha than those observed in control samples (mean 31.20 pg/ml; P<0.05). Both HIV-1-infected and uninfected individuals heterozygous for the delta32ccr5 allele had significantly lower concentrations of circulating RANTES (mean 16.07 and 6.11 ng/ml, respectively) than CCR5/CCR5 individuals (mean 28.23 and 16.07 ng/ml, respectively; P<0.05). These findings suggest that the CCR5 allele and ß-chemokine production may affect the immunopathogenesis of HIV-1. |
| first_indexed | 2024-12-12T22:26:38Z |
| format | Article |
| id | doaj.art-33bbaccf5f064e11bc4c24a29d8c2004 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 0034-7310 |
| language | English |
| last_indexed | 2024-12-12T22:26:38Z |
| publishDate | 2002-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-33bbaccf5f064e11bc4c24a29d8c20042022-12-22T00:09:44ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73102002-01-01351113331337CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individualsMikawa A.Y.Tagliavini S.A.Costa P.I.The 32-bp deletion in the HIV-1 co-receptor CCR5 confers a high degree of resistance to HIV-1 infection in homozygous individuals for the deleted allele and partial protection against HIV-1 during disease progression in heterozygotes. Natural ligands for CCR5, MIP-1alpha, MIP-1ß and RANTES, have been shown to inhibit HIV replication in CD4+ T cells. In the present study, we examined the CCR5 genotype by PCR and the plasma levels of RANTES and MIP-1alpha by ELISA among blood donors (N = 26) and among HIV-1-infected individuals (N = 129). The control group consisted of healthy adult volunteers and HIV-1-infected subjects were an asymptomatic and heterogeneous group of individuals with regard to immunologic and virologic markers of HIV-1 disease. The frequency of the CCR5 mutant allele (delta32ccr5) in this population was 0.032; however, no delta32ccr5 homozygote was detected. These results could be related to the intense ethnic admixture of the Brazilian population. There was no correlation between circulating ß-chemokines (MIP-1alpha, RANTES) and viral load in HIV-infected individuals. RANTES concentrations in plasma samples from HIV+ patients carrying the homozygous CCR5 allele (CCR5/CCR5) (28.23 ng/ml) were higher than in the control samples (16.07 ng/ml; P<0.05); however, this HIV+ patient group (mean 26.23 pg/ml) had significantly lower concentrations of MIP-1alpha than those observed in control samples (mean 31.20 pg/ml; P<0.05). Both HIV-1-infected and uninfected individuals heterozygous for the delta32ccr5 allele had significantly lower concentrations of circulating RANTES (mean 16.07 and 6.11 ng/ml, respectively) than CCR5/CCR5 individuals (mean 28.23 and 16.07 ng/ml, respectively; P<0.05). These findings suggest that the CCR5 allele and ß-chemokine production may affect the immunopathogenesis of HIV-1.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100011HIV-1CC chemokine receptor 5delta32ccr5 Frequency alleleChemokines |
| spellingShingle | Mikawa A.Y. Tagliavini S.A. Costa P.I. CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals Brazilian Journal of Medical and Biological Research HIV-1 CC chemokine receptor 5 delta32ccr5 Frequency allele Chemokines |
| title | CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
| title_full | CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
| title_fullStr | CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
| title_full_unstemmed | CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
| title_short | CCR5 genotype and plasma ß-chemokine concentration of Brazilian HIV-infected individuals |
| title_sort | ccr5 genotype and plasma ss chemokine concentration of brazilian hiv infected individuals |
| topic | HIV-1 CC chemokine receptor 5 delta32ccr5 Frequency allele Chemokines |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100011 |
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