Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice
Abiotic stress confers serious damage to the photosynthetic machinery, often resulting in plant growth inhibition. Hypothetical chloroplast open reading frame 3 (Ycf3)-interacting protein 1 (Y3IP1) is a nucleus-encoded thylakoid protein and plays an essential role in the assembly of photosystem I. T...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-05-01
|
| Series: | Rice Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S167263082200021X |
| _version_ | 1828844741034246144 |
|---|---|
| author | Hyeran Moon Young-Ah Kim Ryoung Shin Chang-Jin Park |
| author_facet | Hyeran Moon Young-Ah Kim Ryoung Shin Chang-Jin Park |
| author_sort | Hyeran Moon |
| collection | DOAJ |
| description | Abiotic stress confers serious damage to the photosynthetic machinery, often resulting in plant growth inhibition. Hypothetical chloroplast open reading frame 3 (Ycf3)-interacting protein 1 (Y3IP1) is a nucleus-encoded thylakoid protein and plays an essential role in the assembly of photosystem I. The full-length cDNA over-expresser (FOX) gene-hunting system is an approach using systemically generated gain-of-function mutants. Among the FOX-rice lines, a line CE175 overexpressing rice Y3IP1 gene (OsY3IP1) displayed less inhibition of root growth under saline (NaCl) stress. The expression of OsY3IP1 was up-regulated under saline and alkaline (Na2CO3) stresses in the rice variety Kitaake. After saline and alkaline treatments, transgenic Kitaake overexpressing OsY3IP1-GFP (OsY3IP1-GFPox/Kit) displayed higher levels of chlorophyll content compared to Kitaake. Under the stress conditions, the maximum quantum yield of photosystem II photochemistry levels was higher in OsY3IP1-GFPox/Kit than in Kitaake. The increased tolerance conferred by OsY3IP1 overexpression correlated with reduced reactive oxygen species accumulation. Our data provide new insights into the possible role of OsY3IP1 in the pathway suppressing photooxidative damage under stress conditions. These features can be further exploited to improve saline and alkaline tolerances of rice plants in future. |
| first_indexed | 2024-12-12T21:11:57Z |
| format | Article |
| id | doaj.art-33bc2813e509459dbccd295694415cf2 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-12-12T21:11:57Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-33bc2813e509459dbccd295694415cf22022-12-22T00:11:51ZengElsevierRice Science1672-63082022-05-01293225236Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in RiceHyeran Moon0Young-Ah Kim1Ryoung Shin2Chang-Jin Park3Department of Molecular Biology, Sejong University, Seoul 05006, South KoreaDepartment of Bioresources Engineering, Sejong University, Seoul 05006, South KoreaRIKEN Center for Sustainable Resource Science, Yokohama 230-0045, JapanDepartment of Molecular Biology, Sejong University, Seoul 05006, South Korea; Department of Bioresources Engineering, Sejong University, Seoul 05006, South Korea; Plant Engineering Research Institute, Sejong University, Seoul 05006, South Korea; Corresponding author: Department of Molecular Biology, Sejong University, Seoul 05006, South Korea.Abiotic stress confers serious damage to the photosynthetic machinery, often resulting in plant growth inhibition. Hypothetical chloroplast open reading frame 3 (Ycf3)-interacting protein 1 (Y3IP1) is a nucleus-encoded thylakoid protein and plays an essential role in the assembly of photosystem I. The full-length cDNA over-expresser (FOX) gene-hunting system is an approach using systemically generated gain-of-function mutants. Among the FOX-rice lines, a line CE175 overexpressing rice Y3IP1 gene (OsY3IP1) displayed less inhibition of root growth under saline (NaCl) stress. The expression of OsY3IP1 was up-regulated under saline and alkaline (Na2CO3) stresses in the rice variety Kitaake. After saline and alkaline treatments, transgenic Kitaake overexpressing OsY3IP1-GFP (OsY3IP1-GFPox/Kit) displayed higher levels of chlorophyll content compared to Kitaake. Under the stress conditions, the maximum quantum yield of photosystem II photochemistry levels was higher in OsY3IP1-GFPox/Kit than in Kitaake. The increased tolerance conferred by OsY3IP1 overexpression correlated with reduced reactive oxygen species accumulation. Our data provide new insights into the possible role of OsY3IP1 in the pathway suppressing photooxidative damage under stress conditions. These features can be further exploited to improve saline and alkaline tolerances of rice plants in future.http://www.sciencedirect.com/science/article/pii/S167263082200021Xalkalinereactive oxygen speciesricesalinestress tolerance |
| spellingShingle | Hyeran Moon Young-Ah Kim Ryoung Shin Chang-Jin Park Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice Rice Science alkaline reactive oxygen species rice saline stress tolerance |
| title | Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice |
| title_full | Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice |
| title_fullStr | Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice |
| title_full_unstemmed | Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice |
| title_short | Nucleus-Encoded Thylakoid Protein, OsY3IP1, Confers Enhanced Tolerance to Saline and Alkaline Stresses in Rice |
| title_sort | nucleus encoded thylakoid protein osy3ip1 confers enhanced tolerance to saline and alkaline stresses in rice |
| topic | alkaline reactive oxygen species rice saline stress tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S167263082200021X |
| work_keys_str_mv | AT hyeranmoon nucleusencodedthylakoidproteinosy3ip1confersenhancedtolerancetosalineandalkalinestressesinrice AT youngahkim nucleusencodedthylakoidproteinosy3ip1confersenhancedtolerancetosalineandalkalinestressesinrice AT ryoungshin nucleusencodedthylakoidproteinosy3ip1confersenhancedtolerancetosalineandalkalinestressesinrice AT changjinpark nucleusencodedthylakoidproteinosy3ip1confersenhancedtolerancetosalineandalkalinestressesinrice |