Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesis
As the concerned emerging pollutants, several lines of evidence have indicated that nanoplastics (NPs) lead to reproductive toxicity. However, the biological mechanism underlying NPs disturbed spermatogenesis remains largely unknown. Therefore, we aimed to reveal the potential mechanism of impaired...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323011302 |
| _version_ | 1797648350423547904 |
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| author | Yan Zhou Xiang Zhong Liangkai Chen Liming Gong Lei Luo Qian He Lin Zhu Kunming Tian |
| author_facet | Yan Zhou Xiang Zhong Liangkai Chen Liming Gong Lei Luo Qian He Lin Zhu Kunming Tian |
| author_sort | Yan Zhou |
| collection | DOAJ |
| description | As the concerned emerging pollutants, several lines of evidence have indicated that nanoplastics (NPs) lead to reproductive toxicity. However, the biological mechanism underlying NPs disturbed spermatogenesis remains largely unknown. Therefore, we aimed to reveal the potential mechanism of impaired spermatogenesis caused by long-term NPs exposure from the perspective of integrated metabolome and microbiome analysis. After 12 weeks of gavage of polystyrene nanoplastics (PS-NPs) and animo-modified polystyrene nanoplastics (Amino-NPs), a well-designed two-exposure stages experimental condition. We found that NPs exposure induced apparent abnormal spermatogenesis, which appeared more severe in the Amino-NPs group. Mechanistically, 14 floras associated with glucose and lipid metabolism were significantly altered, as evidenced by 16 S rRNA sequencing. Testicular metabolome revealed that the Top 50 changed metabolites were also enriched in lipid metabolism. Subsequently, the combined gut microbiome and metabolome analysis uncovered the strong correlations between Klebsiella, Blautia, Parabacteroides, and lipid metabolites (e.g., PC, LysoPC and GPCho). We speculate that the dysbiosis of gut microbiota-related disturbed lipid metabolism may be responsible for long-term NPs-induced damaged spermatogenesis, which provides new insights into NPs-induced dysregulated spermatogenesis. |
| first_indexed | 2024-03-11T15:30:59Z |
| format | Article |
| id | doaj.art-33bdf9ca9a024f36a5f5d828a2779532 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-11T15:30:59Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-33bdf9ca9a024f36a5f5d828a27795322023-10-27T04:23:42ZengElsevierEcotoxicology and Environmental Safety0147-65132023-11-01267115626Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesisYan Zhou0Xiang Zhong1Liangkai Chen2Liming Gong3Lei Luo4Qian He5Lin Zhu6Kunming Tian7Department of Occupational and Environmental Health, School of Public Health, Zunyi Medical University, Zunyi 563000, PR ChinaDepartment of Gastroenterology, Affiliated Hang Tian Hospital, Zunyi Medical University, Zunyi 563000, PR ChinaDepartment of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, ChinaDepartment of Gynaecology and Obstetrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, PR ChinaDepartment of Occupational and Environmental Health, School of Public Health, Zunyi Medical University, Zunyi 563000, PR ChinaDepartment of Gynaecology and Obstetrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, PR ChinaAffiliated Hospital of Shijiazhuang Medical College, Shijiazhuang 050000, PR ChinaDepartment of Occupational and Environmental Health, School of Public Health, Zunyi Medical University, Zunyi 563000, PR China; Department of Gynaecology and Obstetrics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, PR China; Key Laboratory of Maternal& Child Health and Exposure Science of Guizhou Higher Education Institutes, Zunyi Medical University, Zunyi 563000, PR China; Correspondence to: Department of Occupational and Environmental Health, School of Public Health, Zunyi Medical University, Zunyi 563000, Guizhou, China.As the concerned emerging pollutants, several lines of evidence have indicated that nanoplastics (NPs) lead to reproductive toxicity. However, the biological mechanism underlying NPs disturbed spermatogenesis remains largely unknown. Therefore, we aimed to reveal the potential mechanism of impaired spermatogenesis caused by long-term NPs exposure from the perspective of integrated metabolome and microbiome analysis. After 12 weeks of gavage of polystyrene nanoplastics (PS-NPs) and animo-modified polystyrene nanoplastics (Amino-NPs), a well-designed two-exposure stages experimental condition. We found that NPs exposure induced apparent abnormal spermatogenesis, which appeared more severe in the Amino-NPs group. Mechanistically, 14 floras associated with glucose and lipid metabolism were significantly altered, as evidenced by 16 S rRNA sequencing. Testicular metabolome revealed that the Top 50 changed metabolites were also enriched in lipid metabolism. Subsequently, the combined gut microbiome and metabolome analysis uncovered the strong correlations between Klebsiella, Blautia, Parabacteroides, and lipid metabolites (e.g., PC, LysoPC and GPCho). We speculate that the dysbiosis of gut microbiota-related disturbed lipid metabolism may be responsible for long-term NPs-induced damaged spermatogenesis, which provides new insights into NPs-induced dysregulated spermatogenesis.http://www.sciencedirect.com/science/article/pii/S0147651323011302NanoplasticsSpermatogenesisGut microbiotaLipid metabolismMulti-omics analysis |
| spellingShingle | Yan Zhou Xiang Zhong Liangkai Chen Liming Gong Lei Luo Qian He Lin Zhu Kunming Tian Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesis Ecotoxicology and Environmental Safety Nanoplastics Spermatogenesis Gut microbiota Lipid metabolism Multi-omics analysis |
| title | Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesis |
| title_full | Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesis |
| title_fullStr | Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesis |
| title_full_unstemmed | Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesis |
| title_short | Gut microbiota combined with metabolome dissects long-term nanoplastics exposure-induced disturbed spermatogenesis |
| title_sort | gut microbiota combined with metabolome dissects long term nanoplastics exposure induced disturbed spermatogenesis |
| topic | Nanoplastics Spermatogenesis Gut microbiota Lipid metabolism Multi-omics analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323011302 |
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