| Summary: | Although the conditional gene knockout (KO) is a better choice for observing its phenotype in a specific cell, tissue, and/or organ, the simple null gene KO could nevertheless be attempted initially to scan its overall phenotypes at the level of the whole-body system, especially for a new gene such as <i>Crlz-1</i>. Therefore, with a hope to glean phenotypic clues for <i>Crlz-1</i> at the whole-body system, we attempted to generate its null KO mice. Contrary to our original desire, <i>Crlz-1</i> homozygous null KO mice were not born. However, in the chasing of their homozygous KO embryos, they were found to be lethally impaired from early development, remaining in a state of small globular mass without ever leading to a body shape, indicating the critical role of <i>Crlz-1</i> as a Wnt target gene for the proliferation and/or differentiation of cells during early mouse embryonic development.
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