<i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development
Although the conditional gene knockout (KO) is a better choice for observing its phenotype in a specific cell, tissue, and/or organ, the simple null gene KO could nevertheless be attempted initially to scan its overall phenotypes at the level of the whole-body system, especially for a new gene such...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/3/511 |
| _version_ | 1797471293570809856 |
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| author | Seung-Young Choi Joo-Hyun Pi So-Eun Jeong Chang-Joong Kang |
| author_facet | Seung-Young Choi Joo-Hyun Pi So-Eun Jeong Chang-Joong Kang |
| author_sort | Seung-Young Choi |
| collection | DOAJ |
| description | Although the conditional gene knockout (KO) is a better choice for observing its phenotype in a specific cell, tissue, and/or organ, the simple null gene KO could nevertheless be attempted initially to scan its overall phenotypes at the level of the whole-body system, especially for a new gene such as <i>Crlz-1</i>. Therefore, with a hope to glean phenotypic clues for <i>Crlz-1</i> at the whole-body system, we attempted to generate its null KO mice. Contrary to our original desire, <i>Crlz-1</i> homozygous null KO mice were not born. However, in the chasing of their homozygous KO embryos, they were found to be lethally impaired from early development, remaining in a state of small globular mass without ever leading to a body shape, indicating the critical role of <i>Crlz-1</i> as a Wnt target gene for the proliferation and/or differentiation of cells during early mouse embryonic development. |
| first_indexed | 2024-03-09T19:47:05Z |
| format | Article |
| id | doaj.art-33be8fe5cea642b19aa24a6bd46ad684 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T19:47:05Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-33be8fe5cea642b19aa24a6bd46ad6842023-11-24T01:19:23ZengMDPI AGGenes2073-44252022-03-0113351110.3390/genes13030511<i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early DevelopmentSeung-Young Choi0Joo-Hyun Pi1So-Eun Jeong2Chang-Joong Kang3Department of Genetics and Biotechnology, College of Life Sciences, Kyung Hee University, 1732 Deogyeong-daero, Giheung, Yongin 17104, KoreaDepartment of Genetics and Biotechnology, College of Life Sciences, Kyung Hee University, 1732 Deogyeong-daero, Giheung, Yongin 17104, KoreaDepartment of Genetics and Biotechnology, College of Life Sciences, Kyung Hee University, 1732 Deogyeong-daero, Giheung, Yongin 17104, KoreaDepartment of Genetics and Biotechnology, College of Life Sciences, Kyung Hee University, 1732 Deogyeong-daero, Giheung, Yongin 17104, KoreaAlthough the conditional gene knockout (KO) is a better choice for observing its phenotype in a specific cell, tissue, and/or organ, the simple null gene KO could nevertheless be attempted initially to scan its overall phenotypes at the level of the whole-body system, especially for a new gene such as <i>Crlz-1</i>. Therefore, with a hope to glean phenotypic clues for <i>Crlz-1</i> at the whole-body system, we attempted to generate its null KO mice. Contrary to our original desire, <i>Crlz-1</i> homozygous null KO mice were not born. However, in the chasing of their homozygous KO embryos, they were found to be lethally impaired from early development, remaining in a state of small globular mass without ever leading to a body shape, indicating the critical role of <i>Crlz-1</i> as a Wnt target gene for the proliferation and/or differentiation of cells during early mouse embryonic development.https://www.mdpi.com/2073-4425/13/3/511<i>Crlz-1</i><i>Utp3</i><i>Sas10</i>knockout mouseearly embryonic lethality |
| spellingShingle | Seung-Young Choi Joo-Hyun Pi So-Eun Jeong Chang-Joong Kang <i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development Genes <i>Crlz-1</i> <i>Utp3</i> <i>Sas10</i> knockout mouse early embryonic lethality |
| title | <i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development |
| title_full | <i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development |
| title_fullStr | <i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development |
| title_full_unstemmed | <i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development |
| title_short | <i>Crlz-1</i> Homozygous Null Knockout Mouse Embryos Are Lethally Stopped in Their Early Development |
| title_sort | i crlz 1 i homozygous null knockout mouse embryos are lethally stopped in their early development |
| topic | <i>Crlz-1</i> <i>Utp3</i> <i>Sas10</i> knockout mouse early embryonic lethality |
| url | https://www.mdpi.com/2073-4425/13/3/511 |
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