Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine
In the present study, the various concentrations of AuNP (1.25, 2.5, 5, 10 ppm) were prepared to investigate the biocompatibility, biological performances and cell uptake efficiency via Wharton’s jelly mesenchymal stem cells and rat model. The pure AuNP, AuNP combined with Col (AuNP-Col) and FITC co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/5/1385 |
| _version_ | 1797598654309072896 |
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| author | Wen-Yu Cheng Meng-Yin Yang Chun-An Yeh Yi-Chin Yang Kai-Bo Chang Kai-Yuan Chen Szu-Yuan Liu Chien-Lun Tang Chiung-Chyi Shen Huey-Shan Hung |
| author_facet | Wen-Yu Cheng Meng-Yin Yang Chun-An Yeh Yi-Chin Yang Kai-Bo Chang Kai-Yuan Chen Szu-Yuan Liu Chien-Lun Tang Chiung-Chyi Shen Huey-Shan Hung |
| author_sort | Wen-Yu Cheng |
| collection | DOAJ |
| description | In the present study, the various concentrations of AuNP (1.25, 2.5, 5, 10 ppm) were prepared to investigate the biocompatibility, biological performances and cell uptake efficiency via Wharton’s jelly mesenchymal stem cells and rat model. The pure AuNP, AuNP combined with Col (AuNP-Col) and FITC conjugated AuNP-Col (AuNP-Col-FITC) were characterized by Ultraviolet–visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR) and Dynamic Light Scattering (DLS) assays. For in vitro examinations, we explored whether the Wharton’s jelly MSCs had better viability, higher CXCR4 expression, greater migration distance and lower apoptotic-related proteins expression with AuNP 1.25 and 2.5 ppm treatments. Furthermore, we considered whether the treatments of 1.25 and 2.5 ppm AuNP could induce the CXCR4 knocked down Wharton’s jelly MSCs to express CXCR4 and reduce the expression level of apoptotic proteins. We also treated the Wharton’s jelly MSCs with AuNP-Col to investigate the intracellular uptake mechanisms. The evidence demonstrated the cells uptake AuNP-Col through clathrin-mediated endocytosis and the vacuolar-type H<sup>+</sup>-ATPase pathway with good stability inside the cells to avoid lysosomal degradation as well as better uptake efficiency. Additionally, the results from in vivo examinations elucidated the 2.5 ppm of AuNP attenuated foreign body responses and had better retention efficacy with tissue integrity in animal model. In conclusion, the evidence demonstrates that AuNP shows promise as a biosafe nanodrug delivery system for development of regenerative medicine coupled with Wharton’s jelly MSCs. |
| first_indexed | 2024-03-11T03:24:10Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T03:24:10Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-33c267213d304e7db5b756cee400f7252023-11-18T02:50:50ZengMDPI AGPharmaceutics1999-49232023-04-01155138510.3390/pharmaceutics15051385Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative MedicineWen-Yu Cheng0Meng-Yin Yang1Chun-An Yeh2Yi-Chin Yang3Kai-Bo Chang4Kai-Yuan Chen5Szu-Yuan Liu6Chien-Lun Tang7Chiung-Chyi Shen8Huey-Shan Hung9Department of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanDepartment of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 404333, TaiwanDepartment of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 404333, TaiwanDepartment of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanDepartment of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanDepartment of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanDepartment of Minimally Invasive Skull Base Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung 407204, TaiwanGraduate Institute of Biomedical Science, China Medical University, Taichung 404333, TaiwanIn the present study, the various concentrations of AuNP (1.25, 2.5, 5, 10 ppm) were prepared to investigate the biocompatibility, biological performances and cell uptake efficiency via Wharton’s jelly mesenchymal stem cells and rat model. The pure AuNP, AuNP combined with Col (AuNP-Col) and FITC conjugated AuNP-Col (AuNP-Col-FITC) were characterized by Ultraviolet–visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR) and Dynamic Light Scattering (DLS) assays. For in vitro examinations, we explored whether the Wharton’s jelly MSCs had better viability, higher CXCR4 expression, greater migration distance and lower apoptotic-related proteins expression with AuNP 1.25 and 2.5 ppm treatments. Furthermore, we considered whether the treatments of 1.25 and 2.5 ppm AuNP could induce the CXCR4 knocked down Wharton’s jelly MSCs to express CXCR4 and reduce the expression level of apoptotic proteins. We also treated the Wharton’s jelly MSCs with AuNP-Col to investigate the intracellular uptake mechanisms. The evidence demonstrated the cells uptake AuNP-Col through clathrin-mediated endocytosis and the vacuolar-type H<sup>+</sup>-ATPase pathway with good stability inside the cells to avoid lysosomal degradation as well as better uptake efficiency. Additionally, the results from in vivo examinations elucidated the 2.5 ppm of AuNP attenuated foreign body responses and had better retention efficacy with tissue integrity in animal model. In conclusion, the evidence demonstrates that AuNP shows promise as a biosafe nanodrug delivery system for development of regenerative medicine coupled with Wharton’s jelly MSCs.https://www.mdpi.com/1999-4923/15/5/1385gold nanoparticlemesenchymal stem cellbiological performancenanodrug delivery |
| spellingShingle | Wen-Yu Cheng Meng-Yin Yang Chun-An Yeh Yi-Chin Yang Kai-Bo Chang Kai-Yuan Chen Szu-Yuan Liu Chien-Lun Tang Chiung-Chyi Shen Huey-Shan Hung Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine Pharmaceutics gold nanoparticle mesenchymal stem cell biological performance nanodrug delivery |
| title | Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine |
| title_full | Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine |
| title_fullStr | Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine |
| title_full_unstemmed | Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine |
| title_short | Therapeutic Applications of Mesenchymal Stem Cell Loaded with Gold Nanoparticles for Regenerative Medicine |
| title_sort | therapeutic applications of mesenchymal stem cell loaded with gold nanoparticles for regenerative medicine |
| topic | gold nanoparticle mesenchymal stem cell biological performance nanodrug delivery |
| url | https://www.mdpi.com/1999-4923/15/5/1385 |
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