DNA Replication: From Radioisotopes to Click Chemistry
The replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared n...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-11-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/23/11/3007 |
| _version_ | 1819128335526002688 |
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| author | Anna Ligasová Karel Koberna |
| author_facet | Anna Ligasová Karel Koberna |
| author_sort | Anna Ligasová |
| collection | DOAJ |
| description | The replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared nucleoside and nucleotide analogues that serve as markers of newly synthesized DNA. These analogues are incorporated into the DNA during DNA replication, and are subsequently visualized. Several methods are used for their detection, including the highly popular click chemistry. This review aims to provide the readers with basic information about the various possibilities of the detection of replication activity using nucleoside and nucleotide analogues, and to show the strengths and weaknesses of those different detection systems, including click chemistry for microscopic studies. |
| first_indexed | 2024-12-22T08:26:11Z |
| format | Article |
| id | doaj.art-33c92563d7b14ac780e706dfbc3fc784 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T08:26:11Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-33c92563d7b14ac780e706dfbc3fc7842022-12-21T18:32:37ZengMDPI AGMolecules1420-30492018-11-012311300710.3390/molecules23113007molecules23113007DNA Replication: From Radioisotopes to Click ChemistryAnna Ligasová0Karel Koberna1Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech RepublicFaculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech RepublicThe replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared nucleoside and nucleotide analogues that serve as markers of newly synthesized DNA. These analogues are incorporated into the DNA during DNA replication, and are subsequently visualized. Several methods are used for their detection, including the highly popular click chemistry. This review aims to provide the readers with basic information about the various possibilities of the detection of replication activity using nucleoside and nucleotide analogues, and to show the strengths and weaknesses of those different detection systems, including click chemistry for microscopic studies.https://www.mdpi.com/1420-3049/23/11/3007click chemistrynucleoside and nucleotide analoguesindirect immunocytochemistryisotopes |
| spellingShingle | Anna Ligasová Karel Koberna DNA Replication: From Radioisotopes to Click Chemistry Molecules click chemistry nucleoside and nucleotide analogues indirect immunocytochemistry isotopes |
| title | DNA Replication: From Radioisotopes to Click Chemistry |
| title_full | DNA Replication: From Radioisotopes to Click Chemistry |
| title_fullStr | DNA Replication: From Radioisotopes to Click Chemistry |
| title_full_unstemmed | DNA Replication: From Radioisotopes to Click Chemistry |
| title_short | DNA Replication: From Radioisotopes to Click Chemistry |
| title_sort | dna replication from radioisotopes to click chemistry |
| topic | click chemistry nucleoside and nucleotide analogues indirect immunocytochemistry isotopes |
| url | https://www.mdpi.com/1420-3049/23/11/3007 |
| work_keys_str_mv | AT annaligasova dnareplicationfromradioisotopestoclickchemistry AT karelkoberna dnareplicationfromradioisotopestoclickchemistry |