Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it>
<p>Abstract</p> <p>The majority of filarial nematodes harbour <it>Wolbachia </it>endobacteria, including the major pathogenic species in humans, <it>Onchocerca volvulus</it>, <it>Brugia malayi </it>and <it>Wuchereria bancrofti</it>. T...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-09-01
|
| Series: | Parasites & Vectors |
| Online Access: | http://www.parasitesandvectors.com/content/1/1/31 |
| _version_ | 1818049926095962112 |
|---|---|
| author | Graeff-Teixeira Carlos Ben Renata Johnston Kelly L Ford Louise Kumar Sanjay Foster Jeremy M Taylor Mark J |
| author_facet | Graeff-Teixeira Carlos Ben Renata Johnston Kelly L Ford Louise Kumar Sanjay Foster Jeremy M Taylor Mark J |
| author_sort | Graeff-Teixeira Carlos |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The majority of filarial nematodes harbour <it>Wolbachia </it>endobacteria, including the major pathogenic species in humans, <it>Onchocerca volvulus</it>, <it>Brugia malayi </it>and <it>Wuchereria bancrofti</it>. These obligate endosymbionts have never been demonstrated unequivocally in any non-filariid nematode. However, a recent report described the detection by PCR of <it>Wolbachia </it>in the metastrongylid nematode, <it>Angiostrongylus cantonensis </it>(rat lungworm), a leading cause of eosinophilic meningitis in humans. To address the intriguing possibility of <it>Wolbachia </it>infection in nematode species distinct from the Family Onchocercidae, we used both PCR and immunohistochemistry to screen samples of <it>A. cantonensis </it>and <it>A. costaricensis </it>for the presence of this endosymbiont. We were unable to detect <it>Wolbachia </it>in either species using these methodologies. In addition, bioinformatic and phylogenetic analyses of the <it>Wolbachia </it>gene sequences reported previously from <it>A. cantonensis </it>indicate that they most likely result from contamination with DNA from arthropods and filarial nematodes. This study demonstrates the need for caution in relying solely on PCR for identification of new endosymbiont strains from invertebrate DNA samples.</p> |
| first_indexed | 2024-12-10T10:45:20Z |
| format | Article |
| id | doaj.art-33ca0cf5314740dc8e6094efcd53dba4 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-10T10:45:20Z |
| publishDate | 2008-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-33ca0cf5314740dc8e6094efcd53dba42022-12-22T01:52:11ZengBMCParasites & Vectors1756-33052008-09-01113110.1186/1756-3305-1-31Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it>Graeff-Teixeira CarlosBen RenataJohnston Kelly LFord LouiseKumar SanjayFoster Jeremy MTaylor Mark J<p>Abstract</p> <p>The majority of filarial nematodes harbour <it>Wolbachia </it>endobacteria, including the major pathogenic species in humans, <it>Onchocerca volvulus</it>, <it>Brugia malayi </it>and <it>Wuchereria bancrofti</it>. These obligate endosymbionts have never been demonstrated unequivocally in any non-filariid nematode. However, a recent report described the detection by PCR of <it>Wolbachia </it>in the metastrongylid nematode, <it>Angiostrongylus cantonensis </it>(rat lungworm), a leading cause of eosinophilic meningitis in humans. To address the intriguing possibility of <it>Wolbachia </it>infection in nematode species distinct from the Family Onchocercidae, we used both PCR and immunohistochemistry to screen samples of <it>A. cantonensis </it>and <it>A. costaricensis </it>for the presence of this endosymbiont. We were unable to detect <it>Wolbachia </it>in either species using these methodologies. In addition, bioinformatic and phylogenetic analyses of the <it>Wolbachia </it>gene sequences reported previously from <it>A. cantonensis </it>indicate that they most likely result from contamination with DNA from arthropods and filarial nematodes. This study demonstrates the need for caution in relying solely on PCR for identification of new endosymbiont strains from invertebrate DNA samples.</p>http://www.parasitesandvectors.com/content/1/1/31 |
| spellingShingle | Graeff-Teixeira Carlos Ben Renata Johnston Kelly L Ford Louise Kumar Sanjay Foster Jeremy M Taylor Mark J Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it> Parasites & Vectors |
| title | Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it> |
| title_full | Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it> |
| title_fullStr | Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it> |
| title_full_unstemmed | Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it> |
| title_short | Absence of <it>Wolbachia </it>endobacteria in the non-filariid nematodes <it>Angiostrongylus cantonensis </it>and <it>A. costaricensis</it> |
| title_sort | absence of it wolbachia it endobacteria in the non filariid nematodes it angiostrongylus cantonensis it and it a costaricensis it |
| url | http://www.parasitesandvectors.com/content/1/1/31 |
| work_keys_str_mv | AT graeffteixeiracarlos absenceofitwolbachiaitendobacteriainthenonfilariidnematodesitangiostrongyluscantonensisitanditacostaricensisit AT benrenata absenceofitwolbachiaitendobacteriainthenonfilariidnematodesitangiostrongyluscantonensisitanditacostaricensisit AT johnstonkellyl absenceofitwolbachiaitendobacteriainthenonfilariidnematodesitangiostrongyluscantonensisitanditacostaricensisit AT fordlouise absenceofitwolbachiaitendobacteriainthenonfilariidnematodesitangiostrongyluscantonensisitanditacostaricensisit AT kumarsanjay absenceofitwolbachiaitendobacteriainthenonfilariidnematodesitangiostrongyluscantonensisitanditacostaricensisit AT fosterjeremym absenceofitwolbachiaitendobacteriainthenonfilariidnematodesitangiostrongyluscantonensisitanditacostaricensisit AT taylormarkj absenceofitwolbachiaitendobacteriainthenonfilariidnematodesitangiostrongyluscantonensisitanditacostaricensisit |