Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2
Fusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2. We screened out differentially expressed gene...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/3/180 |
| _version_ | 1811263337070067712 |
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| author | Hua Zhang Xiwen Deng Chuang Zhou Wenda Wu Haibin Zhang |
| author_facet | Hua Zhang Xiwen Deng Chuang Zhou Wenda Wu Haibin Zhang |
| author_sort | Hua Zhang |
| collection | DOAJ |
| description | Fusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2. We screened out differentially expressed genes (DEGs) using RNA-seq and identified 320 upregulated genes and 160 downregulated genes. The enrichment pathways of these DEGs focused on immune-related pathways. DON induced proinflammatory gene expression, including cytokines, chemokines and other inflammation-related genes. DON increased IL1A, IL6 and TNF-α release and DON activated the phosphorylation of extracellular signal-regulated kinase-1 and-2 (ERK1/2), JUN N-terminal kinase (JNK) and p38 MAPK. A p38 inhibitor attenuated DON-induced IL6, TNF-α, CXCL2, CXCL8, IL12A, IL1A, CCL20, CCL4 and IL15 production, while an ERK1/2 inhibitor had only a small inhibitory effect on IL15 and IL6. An inhibitor of p38 MAPK decreased the release of IL1A, IL6 and TNF-α and an inhibitor of ERK1/2 partly attenuated protein levels of IL6. These data demonstrate that DON induces proinflammatory factor production in IPEC-J2 cells by activating p38 and ERK1/2. |
| first_indexed | 2024-04-12T19:43:59Z |
| format | Article |
| id | doaj.art-33cafb0087d4494a9c1fb5ce93e49153 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-12T19:43:59Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-33cafb0087d4494a9c1fb5ce93e491532022-12-22T03:19:00ZengMDPI AGToxins2072-66512020-03-0112318010.3390/toxins12030180toxins12030180Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2Hua Zhang0Xiwen Deng1Chuang Zhou2Wenda Wu3Haibin Zhang4College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Vocational College of Agriculture and Forestry, Jurong 212400, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaFusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2. We screened out differentially expressed genes (DEGs) using RNA-seq and identified 320 upregulated genes and 160 downregulated genes. The enrichment pathways of these DEGs focused on immune-related pathways. DON induced proinflammatory gene expression, including cytokines, chemokines and other inflammation-related genes. DON increased IL1A, IL6 and TNF-α release and DON activated the phosphorylation of extracellular signal-regulated kinase-1 and-2 (ERK1/2), JUN N-terminal kinase (JNK) and p38 MAPK. A p38 inhibitor attenuated DON-induced IL6, TNF-α, CXCL2, CXCL8, IL12A, IL1A, CCL20, CCL4 and IL15 production, while an ERK1/2 inhibitor had only a small inhibitory effect on IL15 and IL6. An inhibitor of p38 MAPK decreased the release of IL1A, IL6 and TNF-α and an inhibitor of ERK1/2 partly attenuated protein levels of IL6. These data demonstrate that DON induces proinflammatory factor production in IPEC-J2 cells by activating p38 and ERK1/2.https://www.mdpi.com/2072-6651/12/3/180deoxynivalenolipec-j2 cellsrna-seqinflammationmapks |
| spellingShingle | Hua Zhang Xiwen Deng Chuang Zhou Wenda Wu Haibin Zhang Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 Toxins deoxynivalenol ipec-j2 cells rna-seq inflammation mapks |
| title | Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 |
| title_full | Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 |
| title_fullStr | Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 |
| title_full_unstemmed | Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 |
| title_short | Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 |
| title_sort | deoxynivalenol induces inflammation in ipec j2 cells by activating p38 mapk and erk1 2 |
| topic | deoxynivalenol ipec-j2 cells rna-seq inflammation mapks |
| url | https://www.mdpi.com/2072-6651/12/3/180 |
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