Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort
Background: Infertility is the inability of a couple to conceive after one and a half years of unprotected sex. Male infertility, which accounts for almost half of infertility cases, is considered as a major problem all over the world. The aim of this study was to investigate the association of CYP1...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2017-10-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45428_dd1afdbb8d1da1f13aabc6d0afcf7564.pdf |
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| author | Shalaka S Ramgir Nishu Sekar Divya Jindam. Abilash V.G. |
| author_facet | Shalaka S Ramgir Nishu Sekar Divya Jindam. Abilash V.G. |
| author_sort | Shalaka S Ramgir |
| collection | DOAJ |
| description | Background: Infertility is the inability of a couple to conceive after one and a half years of unprotected sex. Male infertility, which accounts for almost half of infertility cases, is considered as a major problem all over the world. The aim of this study was to investigate the association of CYP1A1 polymorphisms with idiopathic non-obstructive azoospermia in a South Indian cohort. Materials and Methods: An experimental study was conducted with idiopathic nonobstructive azoospermia. A total of 120 infertile and 80 fertile samples were collected, and DNA was then extracted from all samples. The CYP1A1*2A polymorphism genotyping was carried out by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: The genotype distribution of CYP1A1*2A polymorphism showed significant difference between patients and controls. Moreover, the CC genotype was associated with decreased risk of idiopathic non-obstructive azoospermia in comparison with the TT and TC genotypes. Conclusion: The current experimental study identified that the CT genotype of CYP1A1*2A polymorphism may contribute to the pathogenesis of male infertility in the South Indian population. |
| first_indexed | 2024-12-21T00:01:02Z |
| format | Article |
| id | doaj.art-33cb44d377644b66b86e68dadd1d0566 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-21T00:01:02Z |
| publishDate | 2017-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-33cb44d377644b66b86e68dadd1d05662022-12-21T19:22:35ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782017-10-0111314214710.22074/ijfs.2017.475245428Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian CohortShalaka S Ramgir0Nishu Sekar1Divya Jindam.2Abilash V.G.3Department of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu- 632014, IndiaDepartment of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu- 632014, IndiaDepartment of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu- 632014, IndiaDepartment of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu- 632014, IndiaBackground: Infertility is the inability of a couple to conceive after one and a half years of unprotected sex. Male infertility, which accounts for almost half of infertility cases, is considered as a major problem all over the world. The aim of this study was to investigate the association of CYP1A1 polymorphisms with idiopathic non-obstructive azoospermia in a South Indian cohort. Materials and Methods: An experimental study was conducted with idiopathic nonobstructive azoospermia. A total of 120 infertile and 80 fertile samples were collected, and DNA was then extracted from all samples. The CYP1A1*2A polymorphism genotyping was carried out by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: The genotype distribution of CYP1A1*2A polymorphism showed significant difference between patients and controls. Moreover, the CC genotype was associated with decreased risk of idiopathic non-obstructive azoospermia in comparison with the TT and TC genotypes. Conclusion: The current experimental study identified that the CT genotype of CYP1A1*2A polymorphism may contribute to the pathogenesis of male infertility in the South Indian population.http://www.ijfs.ir/article_45428_dd1afdbb8d1da1f13aabc6d0afcf7564.pdfcyp1a1restriction fragment length polymorphisminfertilitycohort |
| spellingShingle | Shalaka S Ramgir Nishu Sekar Divya Jindam. Abilash V.G. Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort International Journal of Fertility and Sterility cyp1a1 restriction fragment length polymorphism infertility cohort |
| title | Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort |
| title_full | Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort |
| title_fullStr | Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort |
| title_full_unstemmed | Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort |
| title_short | Association of CYP1A1*2A Polymorphism with Idiopathic Non-Obstructive Azoospermia in A South Indian Cohort |
| title_sort | association of cyp1a1 2a polymorphism with idiopathic non obstructive azoospermia in a south indian cohort |
| topic | cyp1a1 restriction fragment length polymorphism infertility cohort |
| url | http://www.ijfs.ir/article_45428_dd1afdbb8d1da1f13aabc6d0afcf7564.pdf |
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