Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in Morocco
The chickpea chlorotic dwarf virus (CpCDV) (from the genus <i>Mastrevirus</i> and the family <i>Geminiviridae</i>) and tomato leaf curl New Delhi virus (ToLCNDV) (from the genus <i>Begomovirus</i> and the family <i>Geminiviridae</i>) represent an impor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/10/927 |
| _version_ | 1797472962347008000 |
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| author | Nabil Radouane Rachid Lahlali Meryem Darif Said Ezrari Meryem Benjelloun Zineb Belabess Essaid Ait Barka Abdessalem Tahiri |
| author_facet | Nabil Radouane Rachid Lahlali Meryem Darif Said Ezrari Meryem Benjelloun Zineb Belabess Essaid Ait Barka Abdessalem Tahiri |
| author_sort | Nabil Radouane |
| collection | DOAJ |
| description | The chickpea chlorotic dwarf virus (CpCDV) (from the genus <i>Mastrevirus</i> and the family <i>Geminiviridae</i>) and tomato leaf curl New Delhi virus (ToLCNDV) (from the genus <i>Begomovirus</i> and the family <i>Geminiviridae</i>) represent an important threat to different crops worldwide, as they are emerging viruses in the Asian continent, were introduced to the Mediterranean region in 2012, and were then reported in Morocco in 2017 and 2018. The present study addresses the dispersion of the CpCDV and the ToLCNDV and evaluates the genetic diversity of the Moroccan isolates of both viruses. A total of 1333 symptomatic leaf plant samples were analyzed by PCR. The study has reported the detection of the ToLCNDV in melon and tomato, as well as the CpCDV in squash crops for the first time in Morocco. Blast analysis of selected representative isolates showed a 97–99% nucleotide identity with the ToLCNDV and the CpCDV infecting different crops in the Mediterranean region. Phylogenetic analysis showed low variability among the Moroccan isolates for the ToLCNDV compared to the Spanish and Italian isolates, whereas the CpCDV strains were variable regarding strains reported in Tunisia and Egypt. Recombination analysis showed the presence of the ToLCNDV recombinant strains with variable parents. The spread of both geminiviruses represents a threat to different crop production, requiring the development of crop protection and management strategies. To prevent viral outbreaks, restrictive phytosanitary measures and the development of resistance strategies are also necessary. |
| first_indexed | 2024-03-09T20:08:30Z |
| format | Article |
| id | doaj.art-33cb5050a5db4306a076ddca4b02ed25 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T20:08:30Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-33cb5050a5db4306a076ddca4b02ed252023-11-24T00:23:28ZengMDPI AGHorticulturae2311-75242022-10-0181092710.3390/horticulturae8100927Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in MoroccoNabil Radouane0Rachid Lahlali1Meryem Darif2Said Ezrari3Meryem Benjelloun4Zineb Belabess5Essaid Ait Barka6Abdessalem Tahiri7Phytopathology Unit, Department of Plant Protection, Ecole Nationale d’Agriculture de Meknès, Km10, Rte Haj Kaddour, BP S/40, Meknès 50001, MoroccoPhytopathology Unit, Department of Plant Protection, Ecole Nationale d’Agriculture de Meknès, Km10, Rte Haj Kaddour, BP S/40, Meknès 50001, MoroccoPhytopathology Unit, Department of Plant Protection, Ecole Nationale d’Agriculture de Meknès, Km10, Rte Haj Kaddour, BP S/40, Meknès 50001, MoroccoPhytopathology Unit, Department of Plant Protection, Ecole Nationale d’Agriculture de Meknès, Km10, Rte Haj Kaddour, BP S/40, Meknès 50001, MoroccoLaboratory of Functional Ecology and Environmental Engineering, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Route d’Imouzzer, Fez 30500, MoroccoPlant Protection Laboratory, Regional Center of Agricultural Research of Oujda, National Institute of Agricultural Research, Avenue Mohamed VI, BP428 Oujda, Oujda 60000, MoroccoUnité de Recherche Résistance Induite et Bio-Protection des Plantes-EA 4707 USC INRAE 1488, Université de Reims Champagne-Ardenne, 51100 Reims, FrancePhytopathology Unit, Department of Plant Protection, Ecole Nationale d’Agriculture de Meknès, Km10, Rte Haj Kaddour, BP S/40, Meknès 50001, MoroccoThe chickpea chlorotic dwarf virus (CpCDV) (from the genus <i>Mastrevirus</i> and the family <i>Geminiviridae</i>) and tomato leaf curl New Delhi virus (ToLCNDV) (from the genus <i>Begomovirus</i> and the family <i>Geminiviridae</i>) represent an important threat to different crops worldwide, as they are emerging viruses in the Asian continent, were introduced to the Mediterranean region in 2012, and were then reported in Morocco in 2017 and 2018. The present study addresses the dispersion of the CpCDV and the ToLCNDV and evaluates the genetic diversity of the Moroccan isolates of both viruses. A total of 1333 symptomatic leaf plant samples were analyzed by PCR. The study has reported the detection of the ToLCNDV in melon and tomato, as well as the CpCDV in squash crops for the first time in Morocco. Blast analysis of selected representative isolates showed a 97–99% nucleotide identity with the ToLCNDV and the CpCDV infecting different crops in the Mediterranean region. Phylogenetic analysis showed low variability among the Moroccan isolates for the ToLCNDV compared to the Spanish and Italian isolates, whereas the CpCDV strains were variable regarding strains reported in Tunisia and Egypt. Recombination analysis showed the presence of the ToLCNDV recombinant strains with variable parents. The spread of both geminiviruses represents a threat to different crop production, requiring the development of crop protection and management strategies. To prevent viral outbreaks, restrictive phytosanitary measures and the development of resistance strategies are also necessary.https://www.mdpi.com/2311-7524/8/10/927PCR assays<i>Begomovirus</i><i>Mastrevirus</i>phylogenyhost range |
| spellingShingle | Nabil Radouane Rachid Lahlali Meryem Darif Said Ezrari Meryem Benjelloun Zineb Belabess Essaid Ait Barka Abdessalem Tahiri Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in Morocco Horticulturae PCR assays <i>Begomovirus</i> <i>Mastrevirus</i> phylogeny host range |
| title | Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in Morocco |
| title_full | Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in Morocco |
| title_fullStr | Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in Morocco |
| title_full_unstemmed | Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in Morocco |
| title_short | Detection and Molecular Characterization of Chickpea Chlorotic Dwarf Virus and Tomato Leaf Curl New Delhi Virus in Morocco |
| title_sort | detection and molecular characterization of chickpea chlorotic dwarf virus and tomato leaf curl new delhi virus in morocco |
| topic | PCR assays <i>Begomovirus</i> <i>Mastrevirus</i> phylogeny host range |
| url | https://www.mdpi.com/2311-7524/8/10/927 |
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