Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment
Objective: The use of biocompatible scaffolds with appropriate characteristics to treat large bone defects has attractedsignificant attention. The main objective of the current study is to fabricate a 3D nanocomposite structure that containsgreen synthesized magnesium oxide nanoparticles (MgONPs) an...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2023-07-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_704577_d5b5279e5b0d516fb284a3ead4c11fa6.pdf |
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| author | Elham Ghanbari Mozafar Khazaei Ahmad Mehdipour Alibaradar Khoshfeterat Behrooz Niknafs |
| author_facet | Elham Ghanbari Mozafar Khazaei Ahmad Mehdipour Alibaradar Khoshfeterat Behrooz Niknafs |
| author_sort | Elham Ghanbari |
| collection | DOAJ |
| description | Objective: The use of biocompatible scaffolds with appropriate characteristics to treat large bone defects has attractedsignificant attention. The main objective of the current study is to fabricate a 3D nanocomposite structure that containsgreen synthesized magnesium oxide nanoparticles (MgONPs) and bacterial cellulose (BC) nanofibres, as a bioscaffoldfor bone regeneration.Materials and Methods: In this experimental study, Camellia sinensis extract was used as the green method tosynthesize MgONPs. The synthesized hydrogels were evaluated for their porosity, morphology, degradation rate,mechanical features, cell attachment, and cytocompatibility. Osteogenic differentiation was assessed by alkalinephosphatase (ALP) activity, real-time reverse transcription-polymerase chain reaction (RT-PCR), and alizarin redstaining.Results: MgONPs significantly increased both mechanical strength (P=0.009) and porosity (P=0.01) of the BChydrogels. Human MG-63 osteoblast proliferation significantly increased in the MgONP-BC group compared to thepure BC group (P=0.003). Expression rates of both the ALP (P=0.001) and osteocalcin (OCN) genes were significantlyenhanced in cells seeded on the MgONP-incorporated BC. MG-63 cells had significantly greater calcium depositionand ALP activity (P=0.002) on the MgONP-BC scaffold compared to the BC at day 21.Conclusion: The MgONP-BC scaffold can promote the osteogenic activity of osteoblast-like cells, which indicates itstherapeutic potential for bone tissue regeneration. |
| first_indexed | 2024-03-12T17:10:03Z |
| format | Article |
| id | doaj.art-33d04db27d344bc597ff95e4adb2d466 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-03-12T17:10:03Z |
| publishDate | 2023-07-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-33d04db27d344bc597ff95e4adb2d4662023-08-06T10:58:43ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142023-07-0125748349510.22074/cellj.2023.1986179.1204704577Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro AssessmentElham Ghanbari0Mozafar Khazaei1Ahmad Mehdipour2Alibaradar Khoshfeterat3Behrooz Niknafs4Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranFertility and Infertility Research Centre, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranFaculty of Chemical Engineering, Sahand University of Technology, Tabriz, IranImmunology Research Centre, Tabriz University of Medical Sciences, Tabriz, IranObjective: The use of biocompatible scaffolds with appropriate characteristics to treat large bone defects has attractedsignificant attention. The main objective of the current study is to fabricate a 3D nanocomposite structure that containsgreen synthesized magnesium oxide nanoparticles (MgONPs) and bacterial cellulose (BC) nanofibres, as a bioscaffoldfor bone regeneration.Materials and Methods: In this experimental study, Camellia sinensis extract was used as the green method tosynthesize MgONPs. The synthesized hydrogels were evaluated for their porosity, morphology, degradation rate,mechanical features, cell attachment, and cytocompatibility. Osteogenic differentiation was assessed by alkalinephosphatase (ALP) activity, real-time reverse transcription-polymerase chain reaction (RT-PCR), and alizarin redstaining.Results: MgONPs significantly increased both mechanical strength (P=0.009) and porosity (P=0.01) of the BChydrogels. Human MG-63 osteoblast proliferation significantly increased in the MgONP-BC group compared to thepure BC group (P=0.003). Expression rates of both the ALP (P=0.001) and osteocalcin (OCN) genes were significantlyenhanced in cells seeded on the MgONP-incorporated BC. MG-63 cells had significantly greater calcium depositionand ALP activity (P=0.002) on the MgONP-BC scaffold compared to the BC at day 21.Conclusion: The MgONP-BC scaffold can promote the osteogenic activity of osteoblast-like cells, which indicates itstherapeutic potential for bone tissue regeneration.https://www.celljournal.org/article_704577_d5b5279e5b0d516fb284a3ead4c11fa6.pdfbacterial cellulosemagnesium oxidenanoparticles osteogenesis |
| spellingShingle | Elham Ghanbari Mozafar Khazaei Ahmad Mehdipour Alibaradar Khoshfeterat Behrooz Niknafs Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment Cell Journal bacterial cellulose magnesium oxide nanoparticles osteogenesis |
| title | Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment |
| title_full | Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment |
| title_fullStr | Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment |
| title_full_unstemmed | Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment |
| title_short | Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment |
| title_sort | green synthesized magnesium oxide nanoparticles reinforce osteogenesis properties of bacterial cellulose scaffolds for bone tissue engineering applications an in vitro assessment |
| topic | bacterial cellulose magnesium oxide nanoparticles osteogenesis |
| url | https://www.celljournal.org/article_704577_d5b5279e5b0d516fb284a3ead4c11fa6.pdf |
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