Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in Rapeseed
Alternative oxidases (AOXs) are the terminal oxidase in the cyanide-resistant respiration pathway in plant mitochondria, which play an important role in abiotic stress and are proposed as a functional marker for high tolerant breeding. In this study, ten AOX genes (BnaAOXs) were identified, and CysI...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019-10-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.119.400366 |
| _version_ | 1819149537146568704 |
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| author | Hongli Yang Linbin Deng Hongfang Liu Shihang Fan Wei Hua Jing Liu |
| author_facet | Hongli Yang Linbin Deng Hongfang Liu Shihang Fan Wei Hua Jing Liu |
| author_sort | Hongli Yang |
| collection | DOAJ |
| description | Alternative oxidases (AOXs) are the terminal oxidase in the cyanide-resistant respiration pathway in plant mitochondria, which play an important role in abiotic stress and are proposed as a functional marker for high tolerant breeding. In this study, ten AOX genes (BnaAOXs) were identified, and CysI and CysII of AOX isoforms were highly conserved in rapeseed. Among them, Bna.AOX1b was mainly expressed in the ovule and displayed varying expression between rapeseed cultivars which showed different salt resistance in seed germination. We identified its mitochondrial localization of this gene. To investigate the function of BnaAOX1b in rapeseed, transgenic rapeseed lines with overexpressed BnaAOX1b were created and seed germination and seedling establishment assays were performed under osmotic, salt, and ABA treatment. The results indicated that overexpression of BnaAOX1b significantly improved seed germination under osmotic and salt stress and weakened ABA sensitivity. In addition, post-germination seedling growth was improved under high salt condition, but showed hypersensitivity to ABA. RNA-sequencing analysis indicated that the genes involved in electron transport or energy pathway were induced and a number of gene responses to salt stress and ABA were regulated in Bna.AOX1b overexpressing seeds. Taken together, our results imply that Bna.AOX1b confers tolerance to osmotic and salt stress in terms of seed germination and seedling establishment by regulating stress responsive genes and the response to ABA, and could be utilized as a candidate gene in transgenic breeding. |
| first_indexed | 2024-12-22T14:03:11Z |
| format | Article |
| id | doaj.art-33d085292b8e43b0aa293fbb84385e79 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-22T14:03:11Z |
| publishDate | 2019-10-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-33d085292b8e43b0aa293fbb84385e792022-12-21T18:23:20ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362019-10-019103501351110.1534/g3.119.40036636Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in RapeseedHongli YangLinbin DengHongfang LiuShihang FanWei HuaJing LiuAlternative oxidases (AOXs) are the terminal oxidase in the cyanide-resistant respiration pathway in plant mitochondria, which play an important role in abiotic stress and are proposed as a functional marker for high tolerant breeding. In this study, ten AOX genes (BnaAOXs) were identified, and CysI and CysII of AOX isoforms were highly conserved in rapeseed. Among them, Bna.AOX1b was mainly expressed in the ovule and displayed varying expression between rapeseed cultivars which showed different salt resistance in seed germination. We identified its mitochondrial localization of this gene. To investigate the function of BnaAOX1b in rapeseed, transgenic rapeseed lines with overexpressed BnaAOX1b were created and seed germination and seedling establishment assays were performed under osmotic, salt, and ABA treatment. The results indicated that overexpression of BnaAOX1b significantly improved seed germination under osmotic and salt stress and weakened ABA sensitivity. In addition, post-germination seedling growth was improved under high salt condition, but showed hypersensitivity to ABA. RNA-sequencing analysis indicated that the genes involved in electron transport or energy pathway were induced and a number of gene responses to salt stress and ABA were regulated in Bna.AOX1b overexpressing seeds. Taken together, our results imply that Bna.AOX1b confers tolerance to osmotic and salt stress in terms of seed germination and seedling establishment by regulating stress responsive genes and the response to ABA, and could be utilized as a candidate gene in transgenic breeding.http://g3journal.org/lookup/doi/10.1534/g3.119.400366AOX1bosmotic stresssalt stressabscisic acidrapeseed |
| spellingShingle | Hongli Yang Linbin Deng Hongfang Liu Shihang Fan Wei Hua Jing Liu Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in Rapeseed G3: Genes, Genomes, Genetics AOX1b osmotic stress salt stress abscisic acid rapeseed |
| title | Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in Rapeseed |
| title_full | Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in Rapeseed |
| title_fullStr | Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in Rapeseed |
| title_full_unstemmed | Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in Rapeseed |
| title_short | Overexpression of BnaAOX1b Confers Tolerance to Osmotic and Salt Stress in Rapeseed |
| title_sort | overexpression of bnaaox1b confers tolerance to osmotic and salt stress in rapeseed |
| topic | AOX1b osmotic stress salt stress abscisic acid rapeseed |
| url | http://g3journal.org/lookup/doi/10.1534/g3.119.400366 |
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