Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells
Abstract Background Glioblastoma (GBM) is more difficult to treat than other intractable adult tumors. The main reason that GBM is so difficult to treat is that it is highly infiltrative. Migrasomes are newly discovered membrane structures observed in migrating cells. Thus, they can be generated fro...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
|
| Series: | BMC Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12915-024-01829-w |
| _version_ | 1797273232945971200 |
|---|---|
| author | Seon Yong Lee Sang-Hun Choi Yoonji Kim Hee-Sung Ahn Young-Gyu Ko Kyunggon Kim Sung Wook Chi Hyunggee Kim |
| author_facet | Seon Yong Lee Sang-Hun Choi Yoonji Kim Hee-Sung Ahn Young-Gyu Ko Kyunggon Kim Sung Wook Chi Hyunggee Kim |
| author_sort | Seon Yong Lee |
| collection | DOAJ |
| description | Abstract Background Glioblastoma (GBM) is more difficult to treat than other intractable adult tumors. The main reason that GBM is so difficult to treat is that it is highly infiltrative. Migrasomes are newly discovered membrane structures observed in migrating cells. Thus, they can be generated from GBM cells that have the ability to migrate along the brain parenchyma. However, the function of migrasomes has not yet been elucidated in GBM cells. Results Here, we describe the composition and function of migrasomes generated along with GBM cell migration. Proteomic analysis revealed that LC3B-positive autophagosomes were abundant in the migrasomes of GBM cells. An increased number of migrasomes was observed following treatment with chloroquine (CQ) or inhibition of the expression of STX17 and SNAP29, which are involved in autophagosome/lysosome fusion. Furthermore, depletion of ITGA5 or TSPAN4 did not relieve endoplasmic reticulum (ER) stress in cells, resulting in cell death. Conclusions Taken together, our study suggests that increasing the number of autophagosomes, through inhibition of autophagosome/lysosome fusion, generates migrasomes that have the capacity to alleviate cellular stress. |
| first_indexed | 2024-03-07T14:40:34Z |
| format | Article |
| id | doaj.art-33d4aedb482746fcb9bff621b6b7cb6d |
| institution | Directory Open Access Journal |
| issn | 1741-7007 |
| language | English |
| last_indexed | 2024-03-07T14:40:34Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj.art-33d4aedb482746fcb9bff621b6b7cb6d2024-03-05T20:21:48ZengBMCBMC Biology1741-70072024-01-0122111610.1186/s12915-024-01829-wMigrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cellsSeon Yong Lee0Sang-Hun Choi1Yoonji Kim2Hee-Sung Ahn3Young-Gyu Ko4Kyunggon Kim5Sung Wook Chi6Hyunggee Kim7Department of Biotechnology, Korea UniversityDepartment of Biotechnology, Korea UniversityDepartment of Biotechnology, Korea UniversityConvergence Medicine Research Center, Asan Institute for Life Sciences, Asan Medical CenterDepartment of Life Sciences, Korea UniversityDepartment of Convergence Medicine, Asan Medical Center, University of Ulsan College of MedicineDepartment of Life Sciences, Korea UniversityDepartment of Biotechnology, Korea UniversityAbstract Background Glioblastoma (GBM) is more difficult to treat than other intractable adult tumors. The main reason that GBM is so difficult to treat is that it is highly infiltrative. Migrasomes are newly discovered membrane structures observed in migrating cells. Thus, they can be generated from GBM cells that have the ability to migrate along the brain parenchyma. However, the function of migrasomes has not yet been elucidated in GBM cells. Results Here, we describe the composition and function of migrasomes generated along with GBM cell migration. Proteomic analysis revealed that LC3B-positive autophagosomes were abundant in the migrasomes of GBM cells. An increased number of migrasomes was observed following treatment with chloroquine (CQ) or inhibition of the expression of STX17 and SNAP29, which are involved in autophagosome/lysosome fusion. Furthermore, depletion of ITGA5 or TSPAN4 did not relieve endoplasmic reticulum (ER) stress in cells, resulting in cell death. Conclusions Taken together, our study suggests that increasing the number of autophagosomes, through inhibition of autophagosome/lysosome fusion, generates migrasomes that have the capacity to alleviate cellular stress.https://doi.org/10.1186/s12915-024-01829-wAutophagosomeER stressMigrasomeRetraction fiberCell deathITGA5 |
| spellingShingle | Seon Yong Lee Sang-Hun Choi Yoonji Kim Hee-Sung Ahn Young-Gyu Ko Kyunggon Kim Sung Wook Chi Hyunggee Kim Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells BMC Biology Autophagosome ER stress Migrasome Retraction fiber Cell death ITGA5 |
| title | Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells |
| title_full | Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells |
| title_fullStr | Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells |
| title_full_unstemmed | Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells |
| title_short | Migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells |
| title_sort | migrasomal autophagosomes relieve endoplasmic reticulum stress in glioblastoma cells |
| topic | Autophagosome ER stress Migrasome Retraction fiber Cell death ITGA5 |
| url | https://doi.org/10.1186/s12915-024-01829-w |
| work_keys_str_mv | AT seonyonglee migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells AT sanghunchoi migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells AT yoonjikim migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells AT heesungahn migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells AT younggyuko migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells AT kyunggonkim migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells AT sungwookchi migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells AT hyunggeekim migrasomalautophagosomesrelieveendoplasmicreticulumstressinglioblastomacells |