Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt Stress
The oleaginous microalgae species <i>Chlorococcum sphacosum</i> GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of <i>Chlorococcum s...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| author | Hang Su Jia Feng Junping Lv Qi Liu Fangru Nan Xudong Liu Shulian Xie |
| author_facet | Hang Su Jia Feng Junping Lv Qi Liu Fangru Nan Xudong Liu Shulian Xie |
| author_sort | Hang Su |
| collection | DOAJ |
| description | The oleaginous microalgae species <i>Chlorococcum sphacosum</i> GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of <i>Chlorococcum sphacosum</i> GD were analyzed under salt stress based on transcriptome sequencing. The biomass and lipid content of the alga strain were determined under different NaCl concentrations, and total RNA from fresh cells were isolated and sequenced by HiSeq 2000 high throughput sequencing technology. As the salt concentration increased in culture medium, the algal lipid content increased but the biomass decreased. Following transcriptome sequencing by assembly and splicing, 24,128 unigenes were annotated, with read lengths mostly distributed in the 200–300 bp interval. Statistically significant differentially expressed unigenes were observed in different experimental groups, with 2051 up-regulated genes and 1835 down-regulated genes. The lipid metabolism pathway analysis showed that, under salt stress, gene-related fatty acid biosynthesis (ACCase, KASII, KAR, HAD, FATA) was significantly up-regulated, but some gene-related fatty acid degradation was significantly down-regulated. The comprehensive results showed that salt concentration can affect the lipid accumulation and metabolism of <i>C. sphacosum</i> GD, and the lipid accumulation is closely related to the fatty acid synthesis pathway. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T03:23:20Z |
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| spelling | doaj.art-33d63c928ff64beeb87d28bf70a5c3912023-12-03T15:05:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223130410.3390/ijms22031304Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt StressHang Su0Jia Feng1Junping Lv2Qi Liu3Fangru Nan4Xudong Liu5Shulian Xie6School of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaThe oleaginous microalgae species <i>Chlorococcum sphacosum</i> GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of <i>Chlorococcum sphacosum</i> GD were analyzed under salt stress based on transcriptome sequencing. The biomass and lipid content of the alga strain were determined under different NaCl concentrations, and total RNA from fresh cells were isolated and sequenced by HiSeq 2000 high throughput sequencing technology. As the salt concentration increased in culture medium, the algal lipid content increased but the biomass decreased. Following transcriptome sequencing by assembly and splicing, 24,128 unigenes were annotated, with read lengths mostly distributed in the 200–300 bp interval. Statistically significant differentially expressed unigenes were observed in different experimental groups, with 2051 up-regulated genes and 1835 down-regulated genes. The lipid metabolism pathway analysis showed that, under salt stress, gene-related fatty acid biosynthesis (ACCase, KASII, KAR, HAD, FATA) was significantly up-regulated, but some gene-related fatty acid degradation was significantly down-regulated. The comprehensive results showed that salt concentration can affect the lipid accumulation and metabolism of <i>C. sphacosum</i> GD, and the lipid accumulation is closely related to the fatty acid synthesis pathway.https://www.mdpi.com/1422-0067/22/3/1304<i>Chlorococcum sphacosum</i> GDlipid contenttranscriptome sequencingmetabolic pathway |
| spellingShingle | Hang Su Jia Feng Junping Lv Qi Liu Fangru Nan Xudong Liu Shulian Xie Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt Stress International Journal of Molecular Sciences <i>Chlorococcum sphacosum</i> GD lipid content transcriptome sequencing metabolic pathway |
| title | Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt Stress |
| title_full | Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt Stress |
| title_fullStr | Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt Stress |
| title_full_unstemmed | Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt Stress |
| title_short | Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous <i>Chlorococcum sphacosum</i> GD from Soil under Salt Stress |
| title_sort | molecular mechanism of lipid accumulation and metabolism of oleaginous i chlorococcum sphacosum i gd from soil under salt stress |
| topic | <i>Chlorococcum sphacosum</i> GD lipid content transcriptome sequencing metabolic pathway |
| url | https://www.mdpi.com/1422-0067/22/3/1304 |
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