Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chip
Abstract Vascular endothelial cells play a vital role in the health and maintenance of vascular homeostasis, but hyperglycemia disrupts their function by increasing cellular oxidative stress. Resveratrol, a plant polyphenol, possesses antioxidant properties that can mitigate oxidative stress. Addres...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47291-6 |
| _version_ | 1797397936000204800 |
|---|---|
| author | Farzaneh Fayazbakhsh Fatemeh Hataminia Houra Mobaleghol Eslam Mohammad Ajoudanian Sharmin Kharrazi Kazem Sharifi Hossein Ghanbari |
| author_facet | Farzaneh Fayazbakhsh Fatemeh Hataminia Houra Mobaleghol Eslam Mohammad Ajoudanian Sharmin Kharrazi Kazem Sharifi Hossein Ghanbari |
| author_sort | Farzaneh Fayazbakhsh |
| collection | DOAJ |
| description | Abstract Vascular endothelial cells play a vital role in the health and maintenance of vascular homeostasis, but hyperglycemia disrupts their function by increasing cellular oxidative stress. Resveratrol, a plant polyphenol, possesses antioxidant properties that can mitigate oxidative stress. Addressing the challenges of its limited solubility and stability, gold nanoparticles (GNps) were utilized as carriers. A microfluidic chip (MFC) with dynamic flow conditions was designed to simulate body vessels and to investigate the antioxidant properties of resveratrol gold nanoparticles (RGNps), citrate gold nanoparticles (CGNps), and free Resveratrol on human umbilical vein endothelial cells (HUVEC). The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay was employed to measure the extracellular antioxidant potential, and cell viability was determined using the Alamar Blue test. For assessing intracellular oxidative stress, the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) assay was conducted, and results from both the cell culture plate and MFC were compared. Free Resveratrol demonstrated peak DPPH scavenging activity but had a cell viability of about 24–35%. RGNPs, both 3.0 ± 0.5 nm and 20.2 ± 4.7 nm, consistently showed high cell viability (more than about 90%) across tested concentrations. Notably, RGNPs (20 nm) exhibited antioxidative properties through DPPH scavenging activity (%) in the range of approximately 38–86% which was greater than that of CGNps at about 21–32%. In the MFC,the DCFH-DA analysis indicated that RGNPs (20 nm) reduced cellular oxidative stress by 57–82%, surpassing both CGNps and free Resveratrol. Morphologically, cells in the MFC presented superior structure compared to those in traditional cell culture plates, and the induction of hyperglycemia successfully led to the formation of multinucleated variant endothelial cells (MVECs). The MFC provides a distinct advantage in observing cell morphology and inducing endothelial cell dysfunction. RGNps have demonstrated significant potential in alleviating oxidative stress and preventing endothelial cell disorders. |
| first_indexed | 2024-03-09T01:18:23Z |
| format | Article |
| id | doaj.art-33d66df5ad934f48838682628d16176f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T01:18:23Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-33d66df5ad934f48838682628d16176f2023-12-10T12:18:49ZengNature PortfolioScientific Reports2045-23222023-12-0113111610.1038/s41598-023-47291-6Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chipFarzaneh Fayazbakhsh0Fatemeh Hataminia1Houra Mobaleghol Eslam2Mohammad Ajoudanian3Sharmin Kharrazi4Kazem Sharifi5Hossein Ghanbari6Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesDepartment of Biotechnology and Molecular Medicine, Shahid Beheshti University of Medical SciencesDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesDepartment of Biotechnology and Molecular Medicine, Shahid Beheshti University of Medical SciencesDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesAbstract Vascular endothelial cells play a vital role in the health and maintenance of vascular homeostasis, but hyperglycemia disrupts their function by increasing cellular oxidative stress. Resveratrol, a plant polyphenol, possesses antioxidant properties that can mitigate oxidative stress. Addressing the challenges of its limited solubility and stability, gold nanoparticles (GNps) were utilized as carriers. A microfluidic chip (MFC) with dynamic flow conditions was designed to simulate body vessels and to investigate the antioxidant properties of resveratrol gold nanoparticles (RGNps), citrate gold nanoparticles (CGNps), and free Resveratrol on human umbilical vein endothelial cells (HUVEC). The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay was employed to measure the extracellular antioxidant potential, and cell viability was determined using the Alamar Blue test. For assessing intracellular oxidative stress, the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) assay was conducted, and results from both the cell culture plate and MFC were compared. Free Resveratrol demonstrated peak DPPH scavenging activity but had a cell viability of about 24–35%. RGNPs, both 3.0 ± 0.5 nm and 20.2 ± 4.7 nm, consistently showed high cell viability (more than about 90%) across tested concentrations. Notably, RGNPs (20 nm) exhibited antioxidative properties through DPPH scavenging activity (%) in the range of approximately 38–86% which was greater than that of CGNps at about 21–32%. In the MFC,the DCFH-DA analysis indicated that RGNPs (20 nm) reduced cellular oxidative stress by 57–82%, surpassing both CGNps and free Resveratrol. Morphologically, cells in the MFC presented superior structure compared to those in traditional cell culture plates, and the induction of hyperglycemia successfully led to the formation of multinucleated variant endothelial cells (MVECs). The MFC provides a distinct advantage in observing cell morphology and inducing endothelial cell dysfunction. RGNps have demonstrated significant potential in alleviating oxidative stress and preventing endothelial cell disorders.https://doi.org/10.1038/s41598-023-47291-6 |
| spellingShingle | Farzaneh Fayazbakhsh Fatemeh Hataminia Houra Mobaleghol Eslam Mohammad Ajoudanian Sharmin Kharrazi Kazem Sharifi Hossein Ghanbari Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chip Scientific Reports |
| title | Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chip |
| title_full | Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chip |
| title_fullStr | Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chip |
| title_full_unstemmed | Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chip |
| title_short | Evaluating the antioxidant potential of resveratrol-gold nanoparticles in preventing oxidative stress in endothelium on a chip |
| title_sort | evaluating the antioxidant potential of resveratrol gold nanoparticles in preventing oxidative stress in endothelium on a chip |
| url | https://doi.org/10.1038/s41598-023-47291-6 |
| work_keys_str_mv | AT farzanehfayazbakhsh evaluatingtheantioxidantpotentialofresveratrolgoldnanoparticlesinpreventingoxidativestressinendotheliumonachip AT fatemehhataminia evaluatingtheantioxidantpotentialofresveratrolgoldnanoparticlesinpreventingoxidativestressinendotheliumonachip AT houramobalegholeslam evaluatingtheantioxidantpotentialofresveratrolgoldnanoparticlesinpreventingoxidativestressinendotheliumonachip AT mohammadajoudanian evaluatingtheantioxidantpotentialofresveratrolgoldnanoparticlesinpreventingoxidativestressinendotheliumonachip AT sharminkharrazi evaluatingtheantioxidantpotentialofresveratrolgoldnanoparticlesinpreventingoxidativestressinendotheliumonachip AT kazemsharifi evaluatingtheantioxidantpotentialofresveratrolgoldnanoparticlesinpreventingoxidativestressinendotheliumonachip AT hosseinghanbari evaluatingtheantioxidantpotentialofresveratrolgoldnanoparticlesinpreventingoxidativestressinendotheliumonachip |