Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate
Background: Mesenchymal stem cells (MSCs) are multipotential cells and their therapeutic potency is under intense investigation. Studying the effect of different induction factors on MSCs could increase our knowledge about the differentiation potency of these cells. One of the most important sources...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-04-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/362 |
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| author | Farshad Homayouni Moghadam Tahereh Tayebi Kazem Barzegar |
| author_facet | Farshad Homayouni Moghadam Tahereh Tayebi Kazem Barzegar |
| author_sort | Farshad Homayouni Moghadam |
| collection | DOAJ |
| description | Background: Mesenchymal stem cells (MSCs) are multipotential cells and their therapeutic potency is under intense investigation. Studying the effect of different induction factors on MSCs could increase our knowledge about the differentiation potency of these cells. One of the most important sources of these factors in mammalian body is platelet. Platelet lysate (PL) contains many growth factors and therefore it can be used as a differentiation inducer. In the present study, the effect of PL on differentiation of rat bone marrow MSCs into cardiomyocytes was studied.
Materials Methods: To study the differentiation-inducing effect of PL, MSCs were treated with 2.5, 5 and 10% PL. Early results of this study showed that PL in high concentrations (10%) induces adipogenic differentiation of MSCs. Therefore, to evaluate differentiation to cardiomyocytes, MSCs were cultured in media containing lower levels of PL (2.5% and 5%) and then cardiomyogenic differentiation was induced by treatment with 5-azacytidine. Differentiation of MSCs was evaluated using direct observation of beating cells, immunostaining and real-time PCR techniques.
Results: The results of qPCR showed that treatment with PL alone increased the expression of cardiac alpha actinin (CAA) being predictable by earlier observation of beating cells in PL-treated groups. The results of staining assays against cardiac alpha actinin also showed that there were stained cells in PL-treated groups.
Conclusion: The results of the present study showed that PL is a powerful induction factor for differentiation of MSCs into different cell lines such as cardiomyocytes and adipocytes. |
| first_indexed | 2024-03-12T08:41:39Z |
| format | Article |
| id | doaj.art-33d718daf1084a9a8a22c0e46199a545 |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T08:41:39Z |
| publishDate | 2016-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-33d718daf1084a9a8a22c0e46199a5452023-09-02T16:41:33ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072016-04-01101Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet LysateFarshad Homayouni Moghadam0Tahereh Tayebi1Kazem Barzegar2Neurobiomedical Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranNeurobiomedical Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranHead of the English Language Department, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: Mesenchymal stem cells (MSCs) are multipotential cells and their therapeutic potency is under intense investigation. Studying the effect of different induction factors on MSCs could increase our knowledge about the differentiation potency of these cells. One of the most important sources of these factors in mammalian body is platelet. Platelet lysate (PL) contains many growth factors and therefore it can be used as a differentiation inducer. In the present study, the effect of PL on differentiation of rat bone marrow MSCs into cardiomyocytes was studied. Materials Methods: To study the differentiation-inducing effect of PL, MSCs were treated with 2.5, 5 and 10% PL. Early results of this study showed that PL in high concentrations (10%) induces adipogenic differentiation of MSCs. Therefore, to evaluate differentiation to cardiomyocytes, MSCs were cultured in media containing lower levels of PL (2.5% and 5%) and then cardiomyogenic differentiation was induced by treatment with 5-azacytidine. Differentiation of MSCs was evaluated using direct observation of beating cells, immunostaining and real-time PCR techniques. Results: The results of qPCR showed that treatment with PL alone increased the expression of cardiac alpha actinin (CAA) being predictable by earlier observation of beating cells in PL-treated groups. The results of staining assays against cardiac alpha actinin also showed that there were stained cells in PL-treated groups. Conclusion: The results of the present study showed that PL is a powerful induction factor for differentiation of MSCs into different cell lines such as cardiomyocytes and adipocytes.https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/362Platelet lysateCardiomyogenic differentiation5-azacytidineMesenchymal stem cells |
| spellingShingle | Farshad Homayouni Moghadam Tahereh Tayebi Kazem Barzegar Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate International Journal of Hematology-Oncology and Stem Cell Research Platelet lysate Cardiomyogenic differentiation 5-azacytidine Mesenchymal stem cells |
| title | Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate |
| title_full | Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate |
| title_fullStr | Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate |
| title_full_unstemmed | Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate |
| title_short | Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate |
| title_sort | differentiation of rat bone marrow mesenchymal stem cells into adipocytes and cardiomyocytes after treatment with platelet lysate |
| topic | Platelet lysate Cardiomyogenic differentiation 5-azacytidine Mesenchymal stem cells |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/362 |
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