Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment
AIM: To detect the concentrations of reactive oxygen species (ROS), transient receptor potential mucin-1 (TRPML1), and autophagy-related (Atg) proteins (LC3-I, LC3-II, and Beclin1) in vitreous humor of patients with simple rhegmatogenous retinal detachment (RRD). METHODS: RRD patients enrolled as th...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2023-03-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2023/3/20230303.pdf |
| _version_ | 1811160860399239168 |
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| author | Nan Huang Xue-Yan Gao Jin-Ping Li Xing Lu Hua-Mei Zhu Kai Dong |
| author_facet | Nan Huang Xue-Yan Gao Jin-Ping Li Xing Lu Hua-Mei Zhu Kai Dong |
| author_sort | Nan Huang |
| collection | DOAJ |
| description | AIM: To detect the concentrations of reactive oxygen species (ROS), transient receptor potential mucin-1 (TRPML1), and autophagy-related (Atg) proteins (LC3-I, LC3-II, and Beclin1) in vitreous humor of patients with simple rhegmatogenous retinal detachment (RRD). METHODS: RRD patients enrolled as the RRD group, and patients with idiopathic macular hole (IMH) and idiopathic macular epiretinal membrane (IMEM) were enrolled as control group. The levels of ROS, TRPML1, LC3-I, LC3-II, and Beclin1 in vitreous humor of patients in the RRD and control groups were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: The RRD group included 28 eyes 28 patients and had a higher concentration of ROS in vitreous humor (631.86±18.05 vs 436.34±108.22 IU/mL, P<0.05). The ROS level in patients with a wide retinal detachment (RD) extent (RD range ≥1/2) was higher than that with a narrow RD extent (RD range<1/2, P<0.05). ROS concentration was negatively correlated with RD time (r=-0.46, P=0.01). The expression levels of LC3-I and Beclin1 significantly decreased in RRD (P<0.05), but there were no correlations with the RD time, RD extent, or macular involvement. CONCLUSION: In eyes with RRD, the concentration of ROS in vitreous humor increases and the expression levels of Atg proteins decrease, reflecting possibly that autophagy is inhibited. |
| first_indexed | 2024-04-10T06:05:58Z |
| format | Article |
| id | doaj.art-33d9bc4b6ff541eeb0c6dc85783581a6 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-10T06:05:58Z |
| publishDate | 2023-03-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-33d9bc4b6ff541eeb0c6dc85783581a62023-03-03T03:26:29ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982023-03-0116334835310.18240/ijo.2023.03.0320230303Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachmentNan Huang0Xue-Yan Gao1Jin-Ping Li2Xing Lu3Hua-Mei Zhu4Kai Dong5Kai Dong. Department of Ophthalmology, Eye Center, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, Anhui Province, China. happysubmission@163.com; Hua-Mei Zhu. Hefei Aier Eye Hospital, Hefei 230001, Anhui Province, China. 821252337@qq.comGraduate School of Bengbu Medical College, Bengbu 233000, Anhui Province, ChinaDepartment of Ophthalmology, Eye Center, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, Anhui Province, ChinaGraduate School of Anhui Medical University, Hefei 230001, Anhui Province, ChinaHefei Aier Eye Hospital, Hefei 230001, Anhui Province, ChinaDepartment of Ophthalmology, Eye Center, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, Anhui Province, ChinaAIM: To detect the concentrations of reactive oxygen species (ROS), transient receptor potential mucin-1 (TRPML1), and autophagy-related (Atg) proteins (LC3-I, LC3-II, and Beclin1) in vitreous humor of patients with simple rhegmatogenous retinal detachment (RRD). METHODS: RRD patients enrolled as the RRD group, and patients with idiopathic macular hole (IMH) and idiopathic macular epiretinal membrane (IMEM) were enrolled as control group. The levels of ROS, TRPML1, LC3-I, LC3-II, and Beclin1 in vitreous humor of patients in the RRD and control groups were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: The RRD group included 28 eyes 28 patients and had a higher concentration of ROS in vitreous humor (631.86±18.05 vs 436.34±108.22 IU/mL, P<0.05). The ROS level in patients with a wide retinal detachment (RD) extent (RD range ≥1/2) was higher than that with a narrow RD extent (RD range<1/2, P<0.05). ROS concentration was negatively correlated with RD time (r=-0.46, P=0.01). The expression levels of LC3-I and Beclin1 significantly decreased in RRD (P<0.05), but there were no correlations with the RD time, RD extent, or macular involvement. CONCLUSION: In eyes with RRD, the concentration of ROS in vitreous humor increases and the expression levels of Atg proteins decrease, reflecting possibly that autophagy is inhibited.http://ies.ijo.cn/en_publish/2023/3/20230303.pdfautophagy-related proteinsreactive oxygen speciesvitreous bodyretinal detachment |
| spellingShingle | Nan Huang Xue-Yan Gao Jin-Ping Li Xing Lu Hua-Mei Zhu Kai Dong Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment International Journal of Ophthalmology autophagy-related proteins reactive oxygen species vitreous body retinal detachment |
| title | Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment |
| title_full | Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment |
| title_fullStr | Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment |
| title_full_unstemmed | Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment |
| title_short | Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment |
| title_sort | expression levels of ros and atg proteins in the vitreous in rhegmatogenous retinal detachment |
| topic | autophagy-related proteins reactive oxygen species vitreous body retinal detachment |
| url | http://ies.ijo.cn/en_publish/2023/3/20230303.pdf |
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