Endometrial cytology as an indicator of subclinical endometritis of dairy cattle Holstein Friesian and Jersey breeds
In order to evaluate the presence of polymorphonuclear neutrophils (% PMN-N) as an indicator of endometritis sub clinic in dairy cattle Holstein Friesian and Jersey breeds, by the method of endometrial cytology. 94 dairy cows were sampled, and were grouped by genotypic characteristics as: Group 1: 4...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Spanish |
| Published: |
Asociación Peruana de Reproducción Animal
2016-12-01
|
| Series: | SPERMOVA |
| Subjects: | |
| Online Access: | http://spermova.pe/site2/files/Revistas/Rev.%206%20Vol.%202/10-jReategui_2016-2.pdf |
| Summary: | In order to evaluate the presence of polymorphonuclear neutrophils (% PMN-N) as an indicator of endometritis sub clinic in dairy cattle Holstein Friesian and Jersey breeds, by the method of endometrial cytology. 94 dairy cows were sampled, and were grouped by genotypic characteristics as: Group 1: 47 Holstein Friesian cows; Group 2: 47 Jersey cows, both between 21 and 56 days postpartum. It were evaluated: age, body condition, lactation number, number of birth, date of birth and days in milk to obtain the sample data were evaluated with a test of homogeneity based on statistical Chi square (p <0, 05). From each cow a cytological sample of the endometrial mucosa was took, using adapted cervical brushes. Smears were air dried and set, then were taken to the laboratory to be colored by Diff-Quick staining, to cell reading fields proceed. The neutrophils were used to determine the degree of inflammation of the uterine lining, obtaining a percentage of Polymorph Nuclear neutrophils (PMN-N %), relative to the total cells. The criteria for diagnosing of positive subclinical endometritis (SE) was ≥5,10% of PMN-N (Reátegui et al., 2015) in each smear. The frequency of subclinical endometritis in cows of different genotype did show statistically significant differences (p< 0.05), it is observed that 59.57% of Holstein Friesian cows under study are positive to subclinical endometritis compared to 27.66% of Jersey cows having a marked percentage of SE difference presentation. The frequency of SE of both breeds as lactation, showed that in the 2nd and 4th-feeding showed statistically significant differences (p <0.05). Also it shows that 53.8% of Holstein cows with 2 lactations are positive to subclinical endometritis compared with 11.1% of Jersey cows. Similarly it is observed that 75.0% of the Holstein Friesian 4 lactations are positive subclinical endometritis against any case Jersey cows. The frequency of subclinical endometritis of both breeds by days in milk showed that cows with 34-46 days in milk showed statistically significant differences (p <0.05). It also shows that 64.0% of the Holstein Friesian cows with 34 to 46 days in milk are positive to subclinical endometritis compared to 23.1% of Jersey cows. Garofolo et al., 2013 noted that between the different genetic groups in primiparous cows no significant differences
(p>0.05) was found in any of the variables studied, the% PMN-N reached a range between 0.4% and 4.4%, with an average of 2.2% still below the values indicating the present investigation reports the% PMN-N by genetic group both as multiparous or primiparous cows showed no significant differences between them. It has be concluded that the overall frequency for SE in different genotype cows did show statistically significant differences (p>0.05), however the presence of PMN-N as an indicator of subclinical endometritis in dairy cows of different genotype with 2 and 4 lactations showed differences statistically significant (p<0.05). |
|---|---|
| ISSN: | 2223-9375 2308-4928 |