The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling
Gastrointestinal (GIT) diseases have risen globally in recent years, and early detection of the host’s gut microbiota, typically through fecal material, has become a crucial component for rapid diagnosis of such diseases. Human fecal material is a complex substance composed of undigested macromolecu...
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MDPI AG
2021-02-01
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author | Sheeana Gangadoo Piumie Rajapaksha Pathirannahalage Samuel Cheeseman Yen Thi Hoang Dang Aaron Elbourne Daniel Cozzolino Kay Latham Vi Khanh Truong James Chapman |
author_facet | Sheeana Gangadoo Piumie Rajapaksha Pathirannahalage Samuel Cheeseman Yen Thi Hoang Dang Aaron Elbourne Daniel Cozzolino Kay Latham Vi Khanh Truong James Chapman |
author_sort | Sheeana Gangadoo |
collection | DOAJ |
description | Gastrointestinal (GIT) diseases have risen globally in recent years, and early detection of the host’s gut microbiota, typically through fecal material, has become a crucial component for rapid diagnosis of such diseases. Human fecal material is a complex substance composed of undigested macromolecules and particles, and the processing of such matter is a challenge due to the unstable nature of its products and the complexity of the matrix. The identification of these products can be used as an indication for present and future diseases; however, many researchers focus on one variable or marker looking for specific biomarkers of disease. Therefore, the combination of genomics, transcriptomics, proteomics and metabonomics can give a detailed and complete insight into the gut environment. The proper sample collection, sample preparation and accurate analytical methods play a crucial role in generating precise microbial data and hypotheses in gut microbiome research, as well as multivariate data analysis in determining the gut microbiome functionality in regard to diseases. This review summarizes fecal sample protocols involved in profiling coeliac disease. |
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format | Article |
id | doaj.art-33daba462a544790bdad2770706e9cb4 |
institution | Directory Open Access Journal |
issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-09T00:48:24Z |
publishDate | 2021-02-01 |
publisher | MDPI AG |
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series | International Journal of Molecular Sciences |
spelling | doaj.art-33daba462a544790bdad2770706e9cb42023-12-11T17:19:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224196510.3390/ijms22041965The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut ProfilingSheeana Gangadoo0Piumie Rajapaksha Pathirannahalage1Samuel Cheeseman2Yen Thi Hoang Dang3Aaron Elbourne4Daniel Cozzolino5Kay Latham6Vi Khanh Truong7James Chapman8School of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaCentre for Nutrition and Food Sciences (CNAFS), Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Brisbane, QLD 4072, AustraliaSchool of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, STEM College, RMIT University, Melbourne, VIC 3001, AustraliaGastrointestinal (GIT) diseases have risen globally in recent years, and early detection of the host’s gut microbiota, typically through fecal material, has become a crucial component for rapid diagnosis of such diseases. Human fecal material is a complex substance composed of undigested macromolecules and particles, and the processing of such matter is a challenge due to the unstable nature of its products and the complexity of the matrix. The identification of these products can be used as an indication for present and future diseases; however, many researchers focus on one variable or marker looking for specific biomarkers of disease. Therefore, the combination of genomics, transcriptomics, proteomics and metabonomics can give a detailed and complete insight into the gut environment. The proper sample collection, sample preparation and accurate analytical methods play a crucial role in generating precise microbial data and hypotheses in gut microbiome research, as well as multivariate data analysis in determining the gut microbiome functionality in regard to diseases. This review summarizes fecal sample protocols involved in profiling coeliac disease.https://www.mdpi.com/1422-0067/22/4/1965gut microbiomefecal analysisanalytical techniques |
spellingShingle | Sheeana Gangadoo Piumie Rajapaksha Pathirannahalage Samuel Cheeseman Yen Thi Hoang Dang Aaron Elbourne Daniel Cozzolino Kay Latham Vi Khanh Truong James Chapman The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling International Journal of Molecular Sciences gut microbiome fecal analysis analytical techniques |
title | The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling |
title_full | The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling |
title_fullStr | The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling |
title_full_unstemmed | The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling |
title_short | The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling |
title_sort | multiomics analyses of fecal matrix and its significance to coeliac disease gut profiling |
topic | gut microbiome fecal analysis analytical techniques |
url | https://www.mdpi.com/1422-0067/22/4/1965 |
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