Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway
Roof plate-specific spondin 1 (R-spondin1, RSPO1) is a Wnt/β-catenin signaling pathway activator that binds with Wnt ligands to stimulate the Wnt/β-catenin signaling pathway, which is key to hair regeneration. However, it is not clear whether recombinant RSPO1 (rRSPO1) affects hair regeneration. Her...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
China Science Publishing & Media Ltd.
2023-07-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2023112 |
| _version_ | 1797635884953108480 |
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| author | Chen Yijun Lu Zhujin Feng Jiaxin Chen Zefeng Liu Zejian Wang Xiuqi Yan Huichao Gao Chunqi |
| author_facet | Chen Yijun Lu Zhujin Feng Jiaxin Chen Zefeng Liu Zejian Wang Xiuqi Yan Huichao Gao Chunqi |
| author_sort | Chen Yijun |
| collection | DOAJ |
| description | Roof plate-specific spondin 1 (R-spondin1, RSPO1) is a Wnt/β-catenin signaling pathway activator that binds with Wnt ligands to stimulate the Wnt/β-catenin signaling pathway, which is key to hair regeneration. However, it is not clear whether recombinant RSPO1 (rRSPO1) affects hair regeneration. Here, we treat C57BL/6 male mice with rRSPO1 and investigate the expression of the Wnt/β-catenin signaling pathway and the activation of hair follicle stem cells in the dorsal skin. The mouse skin color score and hair-covered area are determined to describe hair growth, and the skin samples are subjected to H&E staining, western blot analysis and immunofluorescence staining to evaluate hair follicle development and the expressions of Wnt/β-catenin signaling pathway-related proteins. We find that rRSPO1 activates mouse hair follicle stem cells (mHFSCs) and accelerates hair regeneration. rRSPO1 increases the hair-covered area, the number of hair follicles, and the hair follicle diameter and length. Moreover, rRSPO1 enhances the activity of Wnt/β-catenin signaling pathway-related proteins and the expressions of HFSC markers, as well as mHFSC viability. These results indicate that subcutaneous injection of rRSPO1 can improve hair follicle development by activating the Wnt/β-catenin signaling pathway, thereby promoting hair regeneration. This study demonstrates that rRSPO1 has the potential to treat hair loss by activating the Wnt/β-catenin signaling pathway. |
| first_indexed | 2024-03-11T12:26:28Z |
| format | Article |
| id | doaj.art-33dd55d0fb8a4bc9bcb1ae71ef42ea3c |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:26:28Z |
| publishDate | 2023-07-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-33dd55d0fb8a4bc9bcb1ae71ef42ea3c2023-11-06T08:34:44ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452023-07-01551213122110.3724/abbs.202311220d259ccNovel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathwayChen Yijun0Lu Zhujin1Feng Jiaxin2Chen Zefeng3Liu Zejian4Wang Xiuqi5Yan Huichao6Gao Chunqi7[][][][][][][][]Roof plate-specific spondin 1 (R-spondin1, RSPO1) is a Wnt/β-catenin signaling pathway activator that binds with Wnt ligands to stimulate the Wnt/β-catenin signaling pathway, which is key to hair regeneration. However, it is not clear whether recombinant RSPO1 (rRSPO1) affects hair regeneration. Here, we treat C57BL/6 male mice with rRSPO1 and investigate the expression of the Wnt/β-catenin signaling pathway and the activation of hair follicle stem cells in the dorsal skin. The mouse skin color score and hair-covered area are determined to describe hair growth, and the skin samples are subjected to H&E staining, western blot analysis and immunofluorescence staining to evaluate hair follicle development and the expressions of Wnt/β-catenin signaling pathway-related proteins. We find that rRSPO1 activates mouse hair follicle stem cells (mHFSCs) and accelerates hair regeneration. rRSPO1 increases the hair-covered area, the number of hair follicles, and the hair follicle diameter and length. Moreover, rRSPO1 enhances the activity of Wnt/β-catenin signaling pathway-related proteins and the expressions of HFSC markers, as well as mHFSC viability. These results indicate that subcutaneous injection of rRSPO1 can improve hair follicle development by activating the Wnt/β-catenin signaling pathway, thereby promoting hair regeneration. This study demonstrates that rRSPO1 has the potential to treat hair loss by activating the Wnt/β-catenin signaling pathway.https://www.sciengine.com/doi/10.3724/abbs.2023112roof plate-specific spondin 1 (RSPO1)hair follicle stem cellshair regenerationWnt/β-catenin signaling pathwaymice |
| spellingShingle | Chen Yijun Lu Zhujin Feng Jiaxin Chen Zefeng Liu Zejian Wang Xiuqi Yan Huichao Gao Chunqi Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway Acta Biochimica et Biophysica Sinica roof plate-specific spondin 1 (RSPO1) hair follicle stem cells hair regeneration Wnt/β-catenin signaling pathway mice |
| title | Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway |
| title_full | Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway |
| title_fullStr | Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway |
| title_full_unstemmed | Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway |
| title_short | Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway |
| title_sort | novel recombinant r spondin1 promotes hair regeneration by targeting the wnt β catenin signaling pathway |
| topic | roof plate-specific spondin 1 (RSPO1) hair follicle stem cells hair regeneration Wnt/β-catenin signaling pathway mice |
| url | https://www.sciengine.com/doi/10.3724/abbs.2023112 |
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