Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice

Acetolactate synthase (ALS) catalyzes the initial step in the biosynthesis of branched-chain amino acids, and is highly conserved from bacteria to higher plants. ALS is encoded by a single copy gene in rice genome and is a target enzyme of several classes of herbicides. Although ALS mutations confer...

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Main Authors: Zenpei Shimatani, Ushio Fujikura, Hisaki Ishii, Rie Terada, Keiji Nishida, Akihiko Kondo
Format: Article
Language:English
Published: Elsevier 2018-10-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340918309764
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author Zenpei Shimatani
Ushio Fujikura
Hisaki Ishii
Rie Terada
Keiji Nishida
Akihiko Kondo
author_facet Zenpei Shimatani
Ushio Fujikura
Hisaki Ishii
Rie Terada
Keiji Nishida
Akihiko Kondo
author_sort Zenpei Shimatani
collection DOAJ
description Acetolactate synthase (ALS) catalyzes the initial step in the biosynthesis of branched-chain amino acids, and is highly conserved from bacteria to higher plants. ALS is encoded by a single copy gene in rice genome and is a target enzyme of several classes of herbicides. Although ALS mutations conferring herbicide-resistance property to plants are well documented, effect of Imazamox (IMZ) on rice and the mutations in ALS correlated with IMZ tolerance were unclear. In this article, the effect of IMZ on rice calli and seedlings in tissue culture conditions were evaluated. Also, the ALS A96V mutation was confirmed to improve IMZ tolerance of rice calli. Based on these results, ALS-assisted multiplex targeted base editing in rice was demonstrated in combination with Target-AID, a CRISPR/Cas9-cytidine deaminase fusion system [1], [2].
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spelling doaj.art-33ddc0de3aae4e36945a2d9e1d72f9912022-12-22T01:12:19ZengElsevierData in Brief2352-34092018-10-012013251331Herbicide tolerance-assisted multiplex targeted nucleotide substitution in riceZenpei Shimatani0Ushio Fujikura1Hisaki Ishii2Rie Terada3Keiji Nishida4Akihiko Kondo5Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, JapanGraduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, JapanGraduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, JapanGraduate School of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya, Aichi 468-8502, JapanGraduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Corresponding author.Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Corresponding author at: Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.Acetolactate synthase (ALS) catalyzes the initial step in the biosynthesis of branched-chain amino acids, and is highly conserved from bacteria to higher plants. ALS is encoded by a single copy gene in rice genome and is a target enzyme of several classes of herbicides. Although ALS mutations conferring herbicide-resistance property to plants are well documented, effect of Imazamox (IMZ) on rice and the mutations in ALS correlated with IMZ tolerance were unclear. In this article, the effect of IMZ on rice calli and seedlings in tissue culture conditions were evaluated. Also, the ALS A96V mutation was confirmed to improve IMZ tolerance of rice calli. Based on these results, ALS-assisted multiplex targeted base editing in rice was demonstrated in combination with Target-AID, a CRISPR/Cas9-cytidine deaminase fusion system [1], [2].http://www.sciencedirect.com/science/article/pii/S2352340918309764
spellingShingle Zenpei Shimatani
Ushio Fujikura
Hisaki Ishii
Rie Terada
Keiji Nishida
Akihiko Kondo
Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice
Data in Brief
title Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice
title_full Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice
title_fullStr Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice
title_full_unstemmed Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice
title_short Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice
title_sort herbicide tolerance assisted multiplex targeted nucleotide substitution in rice
url http://www.sciencedirect.com/science/article/pii/S2352340918309764
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AT hisakiishii herbicidetoleranceassistedmultiplextargetednucleotidesubstitutioninrice
AT rieterada herbicidetoleranceassistedmultiplextargetednucleotidesubstitutioninrice
AT keijinishida herbicidetoleranceassistedmultiplextargetednucleotidesubstitutioninrice
AT akihikokondo herbicidetoleranceassistedmultiplextargetednucleotidesubstitutioninrice