Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A review
While primary bone malignancies make up just 0.2% of all cancers, osteosarcoma (OS) is the third most common cancer in adolescents. Due to its highly complex and heterogeneous tumor microenvironment (TME), OS has proven difficult to treat. There has been little to no improvement in therapy for this...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
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| Series: | Journal of Bone Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137423000088 |
| _version_ | 1797850287698870272 |
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| author | Dylan D. Thomas Ryan A. Lacinski Brock A. Lindsey |
| author_facet | Dylan D. Thomas Ryan A. Lacinski Brock A. Lindsey |
| author_sort | Dylan D. Thomas |
| collection | DOAJ |
| description | While primary bone malignancies make up just 0.2% of all cancers, osteosarcoma (OS) is the third most common cancer in adolescents. Due to its highly complex and heterogeneous tumor microenvironment (TME), OS has proven difficult to treat. There has been little to no improvement in therapy for this disease over the last 40 years. Even the recent success of immunotherapies in other blood-borne and solid malignancies has not translated to OS. With frequent recurrence and lung metastases continuing to pose a challenge in the clinic, recent advancements in molecular profiling, such as single-cell RNA sequencing (scRNA-seq), have proven useful in identifying novel biomarkers of OS tumors while providing new insight into this TME that could potentially lead to new therapeutic options. This review combines the analyses of over 150,000 cells from 18 lesions ranging from primary, recurrent, and metastatic OS lesions, revealing distinct cellular populations and gene signatures that exist between them. Here, we detail these previous findings and ultimately convey the intratumoral heterogeneity that exists within OS tumor specimens. |
| first_indexed | 2024-04-09T18:57:56Z |
| format | Article |
| id | doaj.art-33df77d98a9341129f960e52ad64028c |
| institution | Directory Open Access Journal |
| issn | 2212-1374 |
| language | English |
| last_indexed | 2024-04-09T18:57:56Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj.art-33df77d98a9341129f960e52ad64028c2023-04-09T05:46:43ZengElsevierJournal of Bone Oncology2212-13742023-04-0139100475Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A reviewDylan D. Thomas0Ryan A. Lacinski1Brock A. Lindsey2Department of Orthopaedics, West Virginia University School of Medicine, Morgantown, WV, United StatesDepartment of Orthopaedics, West Virginia University School of Medicine, Morgantown, WV, United StatesDepartment of Orthopaedics, West Virginia University School of Medicine, Morgantown, WV, United States; Cancer Institute, West Virginia University School of Medicine, Morgantown, WV, United States; Corresponding author at: Department of Orthopaedics, West Virginia University, PO Box 9196, Morgantown, WV 26506-9196, United States.While primary bone malignancies make up just 0.2% of all cancers, osteosarcoma (OS) is the third most common cancer in adolescents. Due to its highly complex and heterogeneous tumor microenvironment (TME), OS has proven difficult to treat. There has been little to no improvement in therapy for this disease over the last 40 years. Even the recent success of immunotherapies in other blood-borne and solid malignancies has not translated to OS. With frequent recurrence and lung metastases continuing to pose a challenge in the clinic, recent advancements in molecular profiling, such as single-cell RNA sequencing (scRNA-seq), have proven useful in identifying novel biomarkers of OS tumors while providing new insight into this TME that could potentially lead to new therapeutic options. This review combines the analyses of over 150,000 cells from 18 lesions ranging from primary, recurrent, and metastatic OS lesions, revealing distinct cellular populations and gene signatures that exist between them. Here, we detail these previous findings and ultimately convey the intratumoral heterogeneity that exists within OS tumor specimens.http://www.sciencedirect.com/science/article/pii/S2212137423000088OsteosarcomaSingle-cellSequencingRecurrenceMetastasisMicroenvironment |
| spellingShingle | Dylan D. Thomas Ryan A. Lacinski Brock A. Lindsey Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A review Journal of Bone Oncology Osteosarcoma Single-cell Sequencing Recurrence Metastasis Microenvironment |
| title | Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A review |
| title_full | Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A review |
| title_fullStr | Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A review |
| title_full_unstemmed | Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A review |
| title_short | Single-cell RNA-seq reveals intratumoral heterogeneity in osteosarcoma patients: A review |
| title_sort | single cell rna seq reveals intratumoral heterogeneity in osteosarcoma patients a review |
| topic | Osteosarcoma Single-cell Sequencing Recurrence Metastasis Microenvironment |
| url | http://www.sciencedirect.com/science/article/pii/S2212137423000088 |
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