Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable Crops

The potential of the method of fluorescence spectroscopy for the detection of damage and diseases of fruits and vegetables was studied. For this purpose, the spectra of fluorescence of healthy and rotten apples and potatoes have been investigated. Excitation of samples was carried out using a contin...

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Main Authors: Tatiana A. Matveyeva, Ruslan M. Sarimov, Alexander V. Simakin, Maxim E. Astashev, Dmitriy E. Burmistrov, Vasily N. Lednev, Pavel A. Sdvizhenskii, Mikhail Ya. Grishin, Sergey M. Pershin, Narek O. Chilingaryan, Natalya A. Semenova, Alexey S. Dorokhov, Sergey V. Gudkov
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/12/7/3391
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author Tatiana A. Matveyeva
Ruslan M. Sarimov
Alexander V. Simakin
Maxim E. Astashev
Dmitriy E. Burmistrov
Vasily N. Lednev
Pavel A. Sdvizhenskii
Mikhail Ya. Grishin
Sergey M. Pershin
Narek O. Chilingaryan
Natalya A. Semenova
Alexey S. Dorokhov
Sergey V. Gudkov
author_facet Tatiana A. Matveyeva
Ruslan M. Sarimov
Alexander V. Simakin
Maxim E. Astashev
Dmitriy E. Burmistrov
Vasily N. Lednev
Pavel A. Sdvizhenskii
Mikhail Ya. Grishin
Sergey M. Pershin
Narek O. Chilingaryan
Natalya A. Semenova
Alexey S. Dorokhov
Sergey V. Gudkov
author_sort Tatiana A. Matveyeva
collection DOAJ
description The potential of the method of fluorescence spectroscopy for the detection of damage and diseases of fruits and vegetables was studied. For this purpose, the spectra of fluorescence of healthy and rotten apples and potatoes have been investigated. Excitation of samples was carried out using a continuous semiconductor laser with a wavelength of 405 nm and a pulsed solid-state laser with a wavelength of 527 nm. Peaks in the region of 600–700 nm in rotten samples were shifted towards shorter wavelengths for most samples in both modes of spectroscopy. The differences in the fluorescence spectra of a healthy and rotten apple surface have been revealed to be in the spectral range of 550–650 nm for 405 nm continuous excitation. When exposed to a laser in a pulsed mode (527 nm), the contribution of the 630 nm peak in the spectrum increases in rotten samples. The observed differences make it possible to use this method for separating samples of healthy and rotten fruits and vegetables. The article paid attention to the influence of many factors such as sample thickness, time after excitation, contamination by soil and dust, cultivar, and location of the probing on fluorescence spectra.
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spelling doaj.art-33e1078a1d1a453eaadac50ba10669402023-11-30T22:55:05ZengMDPI AGApplied Sciences2076-34172022-03-01127339110.3390/app12073391Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable CropsTatiana A. Matveyeva0Ruslan M. Sarimov1Alexander V. Simakin2Maxim E. Astashev3Dmitriy E. Burmistrov4Vasily N. Lednev5Pavel A. Sdvizhenskii6Mikhail Ya. Grishin7Sergey M. Pershin8Narek O. Chilingaryan9Natalya A. Semenova10Alexey S. Dorokhov11Sergey V. Gudkov12Prokhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 109428 Moscow, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 109428 Moscow, RussiaFederal State Budgetary Scientific Institution “Federal Scientific Agroengineering Center VIM”, 109428 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences (GPI RAS), 119991 Moscow, RussiaThe potential of the method of fluorescence spectroscopy for the detection of damage and diseases of fruits and vegetables was studied. For this purpose, the spectra of fluorescence of healthy and rotten apples and potatoes have been investigated. Excitation of samples was carried out using a continuous semiconductor laser with a wavelength of 405 nm and a pulsed solid-state laser with a wavelength of 527 nm. Peaks in the region of 600–700 nm in rotten samples were shifted towards shorter wavelengths for most samples in both modes of spectroscopy. The differences in the fluorescence spectra of a healthy and rotten apple surface have been revealed to be in the spectral range of 550–650 nm for 405 nm continuous excitation. When exposed to a laser in a pulsed mode (527 nm), the contribution of the 630 nm peak in the spectrum increases in rotten samples. The observed differences make it possible to use this method for separating samples of healthy and rotten fruits and vegetables. The article paid attention to the influence of many factors such as sample thickness, time after excitation, contamination by soil and dust, cultivar, and location of the probing on fluorescence spectra.https://www.mdpi.com/2076-3417/12/7/3391fluorescencerot detectionapplepotato
spellingShingle Tatiana A. Matveyeva
Ruslan M. Sarimov
Alexander V. Simakin
Maxim E. Astashev
Dmitriy E. Burmistrov
Vasily N. Lednev
Pavel A. Sdvizhenskii
Mikhail Ya. Grishin
Sergey M. Pershin
Narek O. Chilingaryan
Natalya A. Semenova
Alexey S. Dorokhov
Sergey V. Gudkov
Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable Crops
Applied Sciences
fluorescence
rot detection
apple
potato
title Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable Crops
title_full Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable Crops
title_fullStr Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable Crops
title_full_unstemmed Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable Crops
title_short Using Fluorescence Spectroscopy to Detect Rot in Fruit and Vegetable Crops
title_sort using fluorescence spectroscopy to detect rot in fruit and vegetable crops
topic fluorescence
rot detection
apple
potato
url https://www.mdpi.com/2076-3417/12/7/3391
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