Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>
Implantable medical devices (IMDs) are susceptible to microbial adhesion and biofilm formation, which lead to several clinical complications, including the occurrence of implant-associated infections. Polylactic acid (PLA) and its composites are currently used for the construction of IMDs. In additi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/14/4378 |
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| author | Marta Lima Rita Teixeira-Santos Luciana C. Gomes Sara I. Faria Jesus Valcarcel José Antonio Vázquez Miguel A. Cerqueira Lorenzo Pastrana Ana I. Bourbon Filipe J. Mergulhão |
| author_facet | Marta Lima Rita Teixeira-Santos Luciana C. Gomes Sara I. Faria Jesus Valcarcel José Antonio Vázquez Miguel A. Cerqueira Lorenzo Pastrana Ana I. Bourbon Filipe J. Mergulhão |
| author_sort | Marta Lima |
| collection | DOAJ |
| description | Implantable medical devices (IMDs) are susceptible to microbial adhesion and biofilm formation, which lead to several clinical complications, including the occurrence of implant-associated infections. Polylactic acid (PLA) and its composites are currently used for the construction of IMDs. In addition, chitosan (CS) is a natural polymer that has been widely used in the medical field due to its antimicrobial and antibiofilm properties, which can be dependent on molecular weight (Mw). The present study aims to evaluate the performance of CS-based surfaces of different Mw to inhibit bacterial biofilm formation. For this purpose, CS-based surfaces were produced by dip-coating and the presence of CS and its derivatives onto PLA films, as well surface homogeneity were confirmed by contact angle measurements, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The antimicrobial activity of the functionalized surfaces was evaluated against single- and dual-species biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. Chitosan-based surfaces were able to inhibit the development of single- and dual-species biofilms by reducing the number of total, viable, culturable, and viable but nonculturable cells up to 79%, 90%, 81%, and 96%, respectively, being their activity dependent on chitosan Mw. The effect of CS-based surfaces on the inhibition of biofilm formation was corroborated by biofilm structure analysis using confocal laser scanning microscopy (CLSM), which revealed a decrease in the biovolume and thickness of the biofilm formed on CS-based surfaces compared to PLA. Overall, these results support the potential of low Mw CS for coating polymeric devices such as IMDs where the two bacteria tested are common colonizers and reduce their biofilm formation. |
| first_indexed | 2024-03-10T09:30:18Z |
| format | Article |
| id | doaj.art-33e11b003969430a958e8b7107a663d7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:30:18Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-33e11b003969430a958e8b7107a663d72023-11-22T04:32:55ZengMDPI AGMolecules1420-30492021-07-012614437810.3390/molecules26144378Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>Marta Lima0Rita Teixeira-Santos1Luciana C. Gomes2Sara I. Faria3Jesus Valcarcel4José Antonio Vázquez5Miguel A. Cerqueira6Lorenzo Pastrana7Ana I. Bourbon8Filipe J. Mergulhão9LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalGrupo de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP36208 Vigo, Galicia, SpainGrupo de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP36208 Vigo, Galicia, SpainInternational Iberian Nanotechnology Laboratory, Department of Life Sciences, Av. Mestre José Veiga s/n, 4715-330 Braga, PortugalInternational Iberian Nanotechnology Laboratory, Department of Life Sciences, Av. Mestre José Veiga s/n, 4715-330 Braga, PortugalInternational Iberian Nanotechnology Laboratory, Department of Life Sciences, Av. Mestre José Veiga s/n, 4715-330 Braga, PortugalLEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalImplantable medical devices (IMDs) are susceptible to microbial adhesion and biofilm formation, which lead to several clinical complications, including the occurrence of implant-associated infections. Polylactic acid (PLA) and its composites are currently used for the construction of IMDs. In addition, chitosan (CS) is a natural polymer that has been widely used in the medical field due to its antimicrobial and antibiofilm properties, which can be dependent on molecular weight (Mw). The present study aims to evaluate the performance of CS-based surfaces of different Mw to inhibit bacterial biofilm formation. For this purpose, CS-based surfaces were produced by dip-coating and the presence of CS and its derivatives onto PLA films, as well surface homogeneity were confirmed by contact angle measurements, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The antimicrobial activity of the functionalized surfaces was evaluated against single- and dual-species biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. Chitosan-based surfaces were able to inhibit the development of single- and dual-species biofilms by reducing the number of total, viable, culturable, and viable but nonculturable cells up to 79%, 90%, 81%, and 96%, respectively, being their activity dependent on chitosan Mw. The effect of CS-based surfaces on the inhibition of biofilm formation was corroborated by biofilm structure analysis using confocal laser scanning microscopy (CLSM), which revealed a decrease in the biovolume and thickness of the biofilm formed on CS-based surfaces compared to PLA. Overall, these results support the potential of low Mw CS for coating polymeric devices such as IMDs where the two bacteria tested are common colonizers and reduce their biofilm formation.https://www.mdpi.com/1420-3049/26/14/4378antibiofilm activitychitosanchitosan-polylactic acid surfacesdual-species biofilmsimplantable medical devicespolylactic acid surfaces |
| spellingShingle | Marta Lima Rita Teixeira-Santos Luciana C. Gomes Sara I. Faria Jesus Valcarcel José Antonio Vázquez Miguel A. Cerqueira Lorenzo Pastrana Ana I. Bourbon Filipe J. Mergulhão Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> Molecules antibiofilm activity chitosan chitosan-polylactic acid surfaces dual-species biofilms implantable medical devices polylactic acid surfaces |
| title | Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> |
| title_full | Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> |
| title_fullStr | Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> |
| title_short | Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms of <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> |
| title_sort | development of chitosan based surfaces to prevent single and dual species biofilms of i staphylococcus aureus i and i pseudomonas aeruginosa i |
| topic | antibiofilm activity chitosan chitosan-polylactic acid surfaces dual-species biofilms implantable medical devices polylactic acid surfaces |
| url | https://www.mdpi.com/1420-3049/26/14/4378 |
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