Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization study
BackgroundIncreasing research has implicated the possible effect of gut microbiota (GM) on the prognosis of ischemic stroke (IS). However, the precise causal relationship between GM and functional outcomes after IS remains unestablished.MethodsData on 211 GM taxa from the MiBioGen consortium and dat...
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| Format: | Article |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1346371/full |
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| author | Anning Zhu Peng Li Yuzhou Chu Xiuxiang Wei Jiangna Zhao Longfei Luo Tao Zhang Juntao Yan |
| author_facet | Anning Zhu Peng Li Yuzhou Chu Xiuxiang Wei Jiangna Zhao Longfei Luo Tao Zhang Juntao Yan |
| author_sort | Anning Zhu |
| collection | DOAJ |
| description | BackgroundIncreasing research has implicated the possible effect of gut microbiota (GM) on the prognosis of ischemic stroke (IS). However, the precise causal relationship between GM and functional outcomes after IS remains unestablished.MethodsData on 211 GM taxa from the MiBioGen consortium and data on prognosis of IS from the Genetics of Ischemic Stroke Functional Outcome (GISCOME) network were utilized as summary-level data of exposure and outcome. Four kinds of Mendelian randomization (MR) methods were carried out to ascertain the causal effect of GM on functional outcomes following IS. A reverse MR analysis was performed on the positive taxa identified in the forward MR analysis to determine the direction of causation. In addition, we conducted a comparative MR analysis without adjusting the baseline National Institute of Health Stroke Scale (NIHSS) of post-stroke functional outcomes to enhance confidence of the results obtained in the main analysis.ResultsFour taxa were identified to be related to stroke prognosis in both main and comparative analyses. Specifically, genus Ruminococcaceae UCG005 and the Eubacterium oxidoreducens group showed significantly negative effects on stroke prognosis, while the genus Lachnospiraceae NK4A136 group and Lachnospiraceae UCG004 showed protective effects against stroke prognosis. The reverse MR analysis did not support a causal role of stroke prognosis in GM. No evidence of heterogeneity, horizontal pleiotropy, and outliers was found.ConclusionThis MR study provided evidence that genetically predicted GM had a causal link with post-stroke outcomes. Specific gut microbiota taxa associated with IS prognosis were identified, which may be helpful to clarify the pathogenesis of ischemic stroke and making treatment strategies. |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T12:31:30Z |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-33e39a31a7514107b92ae2a04baaaafa2024-04-08T04:21:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-04-011510.3389/fmicb.2024.13463711346371Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization studyAnning ZhuPeng LiYuzhou ChuXiuxiang WeiJiangna ZhaoLongfei LuoTao ZhangJuntao YanBackgroundIncreasing research has implicated the possible effect of gut microbiota (GM) on the prognosis of ischemic stroke (IS). However, the precise causal relationship between GM and functional outcomes after IS remains unestablished.MethodsData on 211 GM taxa from the MiBioGen consortium and data on prognosis of IS from the Genetics of Ischemic Stroke Functional Outcome (GISCOME) network were utilized as summary-level data of exposure and outcome. Four kinds of Mendelian randomization (MR) methods were carried out to ascertain the causal effect of GM on functional outcomes following IS. A reverse MR analysis was performed on the positive taxa identified in the forward MR analysis to determine the direction of causation. In addition, we conducted a comparative MR analysis without adjusting the baseline National Institute of Health Stroke Scale (NIHSS) of post-stroke functional outcomes to enhance confidence of the results obtained in the main analysis.ResultsFour taxa were identified to be related to stroke prognosis in both main and comparative analyses. Specifically, genus Ruminococcaceae UCG005 and the Eubacterium oxidoreducens group showed significantly negative effects on stroke prognosis, while the genus Lachnospiraceae NK4A136 group and Lachnospiraceae UCG004 showed protective effects against stroke prognosis. The reverse MR analysis did not support a causal role of stroke prognosis in GM. No evidence of heterogeneity, horizontal pleiotropy, and outliers was found.ConclusionThis MR study provided evidence that genetically predicted GM had a causal link with post-stroke outcomes. Specific gut microbiota taxa associated with IS prognosis were identified, which may be helpful to clarify the pathogenesis of ischemic stroke and making treatment strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1346371/fullgut microbiotaischemic strokefunctional outcomecausal relationshipMendelian randomization |
| spellingShingle | Anning Zhu Peng Li Yuzhou Chu Xiuxiang Wei Jiangna Zhao Longfei Luo Tao Zhang Juntao Yan Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization study Frontiers in Microbiology gut microbiota ischemic stroke functional outcome causal relationship Mendelian randomization |
| title | Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization study |
| title_full | Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization study |
| title_fullStr | Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization study |
| title_full_unstemmed | Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization study |
| title_short | Causal effects of gut microbiota on the prognosis of ischemic stroke: evidence from a bidirectional two-sample Mendelian randomization study |
| title_sort | causal effects of gut microbiota on the prognosis of ischemic stroke evidence from a bidirectional two sample mendelian randomization study |
| topic | gut microbiota ischemic stroke functional outcome causal relationship Mendelian randomization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1346371/full |
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